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[183136_at]

Description R05G6.9 /REP_DB=WormBase Gene ID /WP=CE07422 /TR=Q21756 /GB=AAB00626.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 80      6grp_total > 72      4grp_total > 49      6grp_high1_sub > 02_03_04_05      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 549
1 2 3 4 range
A 89.9
(-3)
P 		458.5
(3)
P 		394
(2)
P 		160.2
(-1.9)
P 		369
B 57.2
(-3)
P 		435
(3)
P 		382.5
(2.2)
P 		97.3
(-2.4)
P 		378
C 100.7
(-3)
P 		606.3
(3)
P 		460
(1.3)
P 		106.8
(-2.9)
P 		506
avr 82.6
(-3) 		499.9
(3) 		412.2
(1.7) 		121.4
(-2.4) 		417

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 112.3
(-2.8)
P 		249
(2.8)
P 		202.7
(0.9)
P 		253.3
(3)
P 		218.1
(1.5)
P 		108.6
(-3)
P 		145
B 116.9
(-3)
P 		260.3
(3)
P 		230.2
(1.7)
P 		256.3
(2.8)
P 		187.7
(-0)
P 		126.9
(-2.6)
P 		143
C 74.2
(-3)
P 		284.1
(2.8)
P 		216
(0.9)
P 		292.3
(3)
P 		193.9
(0.3)
P 		103.5
(-2.2)
P 		218
avr 101.1
(-3) 		264.5
(2.9) 		216.3
(1.2) 		267.3
(3) 		199.9
(0.6) 		113
(-2.6) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 32.7
(-2.3)
P 		99.5
(2.7)
P 		103
(3)
P 		23.5
(-3)
P 		80
B 18.3
(-3)
P 		107.4
(3)
P 		88.6
(1.7)
P 		20.7
(-2.8)
P 		89
C 33.3
(-2.4)
P 		174.3
(3)
P 		160.3
(2.5)
P 		18
(-3)
P 		156
avr 28.1
(-2.6) 		127.1
(3) 		117.3
(2.4) 		20.7
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 20.7
(-3)
P 		88.5
(3)
P 		44.1
(-0.9)
P 		64.2
(0.8)
P 		65.8
(1)
P 		26.4
(-2.5)
P 		68
B 40.7
(-3)
P 		86.6
(3)
P 		55.9
(-1)
P 		67.9
(0.6)
P 		76.2
(1.6)
P 		47.3
(-2.1)
P 		46
C 27.5
(-3)
P 		86.7
(3)
P 		55
(-0.2)
P 		58.8
(0.2)
P 		62.7
(0.6)
P 		34.7
(-2.3)
P 		59
avr 29.6
(-3) 		87.3
(3) 		51.7
(-0.7) 		63.6
(0.5) 		68.2
(1) 		36.1
(-2.3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183136_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9584
Sequence Type Exemplar sequence
Representative Public ID R05G6.9 NCBI
Target Description R05G6.9 /REP_DB=WormBase Gene ID /WP=CE07422 /TR=Q21756 /GB=AAB00626.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068814(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068814 NCBI Caenorhabditis elegans R05G6.9 (R05G6.9) mRNA, complete cds. 11/11 None
SNAP00000020439 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7514475:7516211:-1 11/11 None
GENEFINDER00000020452 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7514475:7516211:-1 11/11 None
R05G6.9 ENSEMBL cdna:known chromosome:CEL140:IV:7514381:7516241:-1 gene:R05G6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7514480-7516217(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R05G6.9
Entrez Gene 187624 Entrez gene
SwissProt Q21756 EMBL-EBI
WormBase CE35359 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_501215 R05G6.9 [Caenorhabditis elegans] gb|AAB00626.2| Hypothetical protein R05G6.9 [Caenorhabditis elegans] 0.0
blast CAE61906 Hypothetical protein CBG05898 [Caenorhabditis briggsae] 0.0
blast XP_780459 PREDICTED: similar to notch1 preproprotein [Strongylocentrotus purpuratus] 2.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 8.2E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 3.5E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 1.7E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 9.1E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 9.2E-5
Sequence
Target Sequence 
>C. ELEGANS:183136_AT
cagctccaatcacattctgttcaccaagtgattgtaacaacaaaggtctatgtttgggaa
caaaaaacagtttttcgtgtgcatgtcaaatcggttatactggttcaagatgcgagaaga
ctccagttgcattatgtgattctcgtgattgcagttcaaatggattatgtattggtacaa
aagactcaatgacttgtgcttgttatcttggatattctggtgataaatgtgaaaagatca
ccggaactatgtgcgaagcttctgactgtaattctaatggaatctgtattggaacaaaga
acttgaaatcctgtatctgcgctcctggatattatggatcacggtgtgaatcgagattcg
ctcttctccctggcaccgaaggaatgttctgtgaagctaaagactgtaatggaaatggga
tctgtataggaaacaagcttcttcccatgtgcatttgtgctcctggattcactggtcttc
gttgcgaacttgagccattatgtactggtgcacttcaatgttctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGCTCCAATCACATTCTGTTCACC 539 203 509 Antisense
GAAGACTCCAGTTGCATTATGTGAT 676 339 624 Antisense
ATGACTTGTGCTTGTTATCTTGGAT 145 45 697 Antisense
GAACTATGTGCGAAGCTTCTGACTG 386 349 752 Antisense
AACTTGAAATCCTGTATCTGCGCTC 395 139 808 Antisense
GTATCTGCGCTCCTGGATATTATGG 230 455 821 Antisense
TGTGAATCGAGATTCGCTCTTCTCC 241 557 853 Antisense
TTCTCCCTGGCACCGAAGGAATGTT 187 687 872 Antisense
TCCCATGTGCATTTGTGCTCCTGGA 160 595 951 Antisense
ATTCACTGGTCTTCGTTGCGAACTT 581 11 975 Antisense
GTACTGGTGCACTTCAATGTTCTGG 356 459 1010 Antisense
Sequence Source Affymetrix Proprietary Database