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[183134_at]

Description C11D2.5 /REP_DB=WormBase Gene ID /WP=CE16846 /TR=O45070 /GB=AAC02574.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_high_8_8 > 32      6grp_highest_nonorm > 90      6grp_total > 14      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 236.2
(3)
P 		86.4
(-1.8)
A 		48.4
(-3)
A 		62.8
(-2.5)
A 		188
B 205.5
(3)
P 		65.3
(-3)
P 		87.3
(-2.1)
P 		90.6
(-1.9)
M 		140
C 208.1
(3)
P 		103.2
(-1.8)
P 		77.9
(-3)
P 		92.5
(-2.3)
P 		130
avr 216.6
(3) 		85
(-2.4) 		71.2
(-3) 		82
(-2.6) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 121.4
(-0.4)
P 		196
(3)
P 		165.7
(1.6)
P 		120.6
(-0.5)
P 		123.5
(-0.3)
P 		66
(-3)
P 		130
B 265.3
(3)
P 		202.5
(1.1)
P 		164.9
(-0.1)
P 		119.9
(-1.5)
P 		124.4
(-1.4)
P 		71.7
(-3)
P 		194
C 278.4
(3)
P 		226
(1)
P 		175.8
(-0.9)
P 		160.4
(-1.5)
P 		125.9
(-2.8)
P 		120.4
(-3)
P 		158
avr 221.7
(3) 		208.2
(2.4) 		168.8
(0.7) 		133.6
(-0.9) 		124.6
(-1.3) 		86
(-3) 		136
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 86
(3)
P 		18.8
(-2.3)
A 		12.6
(-2.7)
A 		9.2
(-3)
A 		77
B 65.5
(3)
P 		16.1
(-3)
P 		20.2
(-2.5)
P 		19.3
(-2.6)
M 		49
C 68.9
(3)
P 		29.7
(-1.4)
P 		27.2
(-1.7)
P 		15.6
(-3)
P 		53
avr 73.5
(3) 		21.5
(-2.3) 		20
(-2.5) 		14.7
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 22.4
(-2.3)
P 		69.7
(3)
P 		36.1
(-0.8)
P 		30.6
(-1.4)
P 		37.3
(-0.6)
P 		16.1
(-3)
P 		54
B 92.3
(3)
P 		67.4
(0.7)
P 		40
(-1.8)
P 		31.8
(-2.5)
P 		50.6
(-0.8)
P 		26.7
(-3)
P 		66
C 103.2
(3)
P 		68.9
(0.1)
P 		44.7
(-2)
P 		32.3
(-3)
P 		40.8
(-2.3)
P 		40.3
(-2.3)
P 		71
avr 72.6
(3) 		68.7
(2.5) 		40.3
(-1.3) 		31.6
(-2.5) 		42.9
(-1) 		27.7
(-3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183134_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10980
Sequence Type Exemplar sequence
Representative Public ID C11D2.5 NCBI
Target Description C11D2.5 /REP_DB=WormBase Gene ID /WP=CE16846 /TR=O45070 /GB=AAC02574.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171352(10), NM_171353(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171352 NCBI Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel family member (nca-1) (nca-1) mRNA, complete cds. 10/11 A 
NM_171353 NCBI Caenorhabditis elegans Novel Channel type/putative Nematode CAlcium channel family member (nca-1) (nca-1) mRNA, complete cds. 11/11 A 
SNAP00000030429 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6157427:6158845:-1 10/11 None
GENEFINDER00000030436 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6156964:6179312:-1 11/11 None
C11D2.6d ENSEMBL cdna:known chromosome:CEL140:IV:6157296:6173024:-1 gene:C11D2.6 10/11 A 
C11D2.6c ENSEMBL cdna:known chromosome:CEL140:IV:6157299:6171656:-1 gene:C11D2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177379 4 2 NM_068525 188103_s_at (11)
NM_171352 174211_s_at (11), 183134_at (10), 188103_s_at (11)
NM_171353 174211_s_at (11), 183134_at (11), 188103_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6157432-6160122(-) 100.0 100.0
Public Domain and Genome References
Ensembl C11D2.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU05590 Novel channel type/putative nematode calcium channel protein 1, isoform d [Caenorhabditis elegans] ref|NP_741413.2| Novel Channel type/putative Nematode CAlcium channel family member (nca-1) [Caenorhabditis elegans] 0.0
blast AAS65871 four domain-type voltage-gated ion channel alpha-1 subunit [Caenorhabditis elegans] 0.0
blast AAM81121 Novel channel type/putative nematode calcium channel protein 1, isoform c [Caenorhabditis elegans] ref|NP_741414.1| Novel Channel type/putative Nematode CAlcium channel family member (nca-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 7.8E-28
Pfam IPR005821 EMBL-EBI Ion transport protein 6.5E-34
Pfam IPR005821 EMBL-EBI Ion transport protein 2.8E-37
Pfam IPR005821 EMBL-EBI Ion transport protein 9.0E-30
Pfam IPR005821 EMBL-EBI Ion transport protein 7.8E-28
Pfam IPR005821 EMBL-EBI Ion transport protein 6.5E-34
Pfam IPR005821 EMBL-EBI Ion transport protein 2.8E-37
Pfam IPR005821 EMBL-EBI Ion transport protein 9.0E-30
Pfam IPR005821 EMBL-EBI Ion transport protein 7.8E-28
Pfam IPR005821 EMBL-EBI Ion transport protein 6.5E-34
Pfam IPR005821 EMBL-EBI Ion transport protein 2.8E-37
Pfam IPR005821 EMBL-EBI Ion transport protein 9.0E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741413.2 20 149-171,186-208,229-248,343-365,429-451,466-488,553-575,624-646,924-946,973-995,1002-1021,1066-1088,1150-1169,1184-1206,1261-1283,1298-1320,1333-1352,1367-1384,1404-1426,1490-1512
NP_741414.1 21 138-160,180-199,214-236,257-276,371-393,503-525,540-562,627-649,698-720,1005-1027,1054-1076,1083-1102,1147-1169,1231-1250,1265-1287,1342-1364,1379-1401,1414-1433,1448-1465,1485-1507,1571-1593
C11D2.6d 20 149-171,186-208,229-248,343-365,429-451,466-488,553-575,624-646,924-946,973-995,1002-1021,1066-1088,1150-1169,1184-1206,1261-1283,1298-1320,1333-1352,1367-1384,1404-1426,1490-1512
C11D2.6c 21 138-160,180-199,214-236,257-276,371-393,503-525,540-562,627-649,698-720,1005-1027,1054-1076,1083-1102,1147-1169,1231-1250,1265-1287,1342-1364,1379-1401,1414-1433,1448-1465,1485-1507,1571-1593
GENEFINDER00000030436 20 155-177,192-214,235-254,349-371,478-500,515-537,602-624,673-695,939-961,988-1010,1017-1036,1081-1103,1165-1184,1199-1221,1276-1298,1313-1335,1348-1367,1382-1399,1419-1441,1505-1527
Sequence
Target Sequence 
>C. ELEGANS:183134_AT
catggctggaaggatgtcttcgtaaaatgagaaatccaagtcaaaaagatgcagaaggag
tacttccatttggtggaggacatccggttattcattcatcaggacattcttcaatttcac
atgaggaaactgttgcacaaagacttcgatttgaaaatattcgaggagatagtgttgata
cagaagagacggagagtagtgaagaagaaactccacctccaataaggaagaaggctgctg
ttaagaatcgaagaggaagtattccagatgttctttcgagaactggtctctttcaagaag
ctgctcgaaagttcatggttggtagctcgagtgagaaaaaacaagtcaagctccagctaa
acgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATGGCTGGAAGGATGTCTTCGTAA 659 209 395 Antisense
GGAGTACTTCCATTTGGTGGAGGAC 292 503 451 Antisense
GAGGACATCCGGTTATTCATTCATC 325 405 470 Antisense
TTATTCATTCATCAGGACATTCTTC 279 679 482 Antisense
GGAAACTGTTGCACAAAGACTTCGA 307 527 519 Antisense
AGAAGAAACTCCACCTCCAATAAGG 25 3 597 Antisense
GAAGGCTGCTGTTAAGAATCGAAGA 490 333 624 Antisense
GTTCTTTCGAGAACTGGTCTCTTTC 236 443 664 Antisense
GAAGCTGCTCGAAAGTTCATGGTTG 423 343 691 Antisense
GTTCATGGTTGGTAGCTCGAGTGAG 411 443 705 Antisense
ACAAGTCAAGCTCCAGCTAAACGTC 530 113 735 Antisense
Sequence Source Affymetrix Proprietary Database