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[183127_at]

Description C34B2.2 /REP_DB=WormBase Gene ID /WP=CE16890 /TR=O44956 /GB=AAB97534.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 77      4grp_high_8_8 > 22      6grp_total > 78      4grp_total > 18      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 227
1 2 3 4 range
A 158.6
(-1.5)
P 		202.8
(-0.3)
P 		102.5
(-3)
A 		329.2
(3)
M 		227
B 166
(-3)
P 		211.6
(0.6)
P 		221
(1.4)
P 		241.8
(3)
P 		76
C 155.8
(-3)
P 		186.8
(-1.6)
P 		195.5
(-1.3)
P 		293
(3)
P 		137
avr 160.1
(-3) 		200.4
(-1.1) 		173
(-2.4) 		288
(3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 443
1 2 3 4 5 6 range
A 145.8
(-3)
P 		176.3
(-2.6)
A 		190.5
(-2.4)
P 		258.5
(-1.4)
P 		377.3
(0.2)
P 		576.8
(3)
P 		431
B 314.4
(-0.8)
P 		209.8
(-2.9)
P 		206.8
(-3)
P 		230.4
(-2.5)
P 		257.2
(-2)
P 		496
(3)
P 		289
C 229.7
(-1.9)
P 		224.6
(-1.9)
P 		229.6
(-1.9)
P 		145.4
(-3)
P 		286
(-1.1)
P 		588.1
(3)
P 		443
avr 230
(-2.5) 		203.6
(-3) 		209
(-2.9) 		211.4
(-2.9) 		306.8
(-1.2) 		553.6
(3) 		350
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 57.8
(3)
P 		44
(0.3)
P 		26.8
(-3)
A 		48.2
(1.1)
M 		31
B 52.9
(3)
P 		52.3
(0.9)
P 		51.2
(-3)
P 		51.5
(-1.9)
P 		2
C 51.6
(-2.3)
P 		53.7
(-1.6)
P 		68.1
(3)
P 		49.4
(-3)
P 		19
avr 54.1
(3) 		50
(-1.6) 		48.7
(-3) 		49.7
(-1.9) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 26.9
(-3)
P 		62.7
(-1.1)
A 		41.5
(-2.2)
P 		65.6
(-1)
P 		113.9
(1.6)
P 		140.4
(3)
P 		114
B 109.4
(-0.4)
P 		69.8
(-2.1)
P 		50.2
(-3)
P 		61
(-2.5)
P 		104.5
(-0.6)
P 		184.9
(3)
P 		135
C 85.1
(-1)
P 		68.5
(-1.6)
P 		58.4
(-2)
P 		29.2
(-3)
P 		92.6
(-0.7)
P 		196.9
(3)
P 		168
avr 73.8
(-1.8) 		67
(-2.2) 		50
(-3) 		51.9
(-2.9) 		103.7
(-0.4) 		174.1
(3) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183127_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.560
Sequence Type Exemplar sequence
Representative Public ID C34B2.2 NCBI
Target Description C34B2.2 /REP_DB=WormBase Gene ID /WP=CE16890 /TR=O44956 /GB=AAB97534.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060399(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060399 NCBI Caenorhabditis elegans KNL (kinetochore null) Binding Protein family member (kbp-5) (kbp-5) mRNA, complete cds. 11/11 None
SNAP00000007249 ENSEMBL cdna:SNAP chromosome:CEL140:I:10695491:10696043:1 11/11 None
GENEFINDER00000007253 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10695491:10696043:1 11/11 None
C34B2.2 ENSEMBL cdna:known chromosome:CEL140:I:10695486:10696180:1 gene:C34B2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10695421-10695974(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol kbp-5
Ensembl C34B2.2
Entrez Gene 172970 Entrez gene
SwissProt O44956 EMBL-EBI
WormBase CE16890 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB97534 Knl (kinetochore null) binding protein protein 5 [Caenorhabditis elegans] ref|NP_492800.1| KNL (kinetochore null) Binding Protein family member (kbp-5) [Caenorhabditis elegans] 4.0E-70
blast CAE67344 Hypothetical protein CBG12807 [Caenorhabditis briggsae] 3.0E-24
Sequence
Target Sequence 
>C. ELEGANS:183127_AT
ataatgaaggcctcctcgagctgtacacacaagcaatctcactcgaagctctcaaagacg
ctgagcaattcctcgagaatagtttgctaggaaaagatgcacaagtcgaacgtctactcg
ctcaagttctcatcgaagagcagcaaatcgagacgaatcgcaaaaagatagcacaattga
gaatcgagaaagaagaactggaattcgattgtgcaaatagtgacctgaagtcgttggagc
agatgcaagccgagcgagatgagctccagaggaagtgccaaccggggcagcacactaaat
cg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAATGAAGGCCTCCTCGAGCTGTA 647 23 26 Antisense
GTACACACAAGCAATCTCACTCGAA 231 461 48 Antisense
TCTCACTCGAAGCTCTCAAAGACGC 343 611 62 Antisense
AAGACGCTGAGCAATTCCTCGAGAA 701 153 80 Antisense
TGCACAAGTCGAACGTCTACTCGCT 41 577 123 Antisense
TCTACTCGCTCAAGTTCTCATCGAA 168 615 138 Antisense
AGTGACCTGAAGTCGTTGGAGCAGA 557 57 244 Antisense
GGAGCAGATGCAAGCCGAGCGAGAT 533 519 261 Antisense
AAGCCGAGCGAGATGAGCTCCAGAG 552 149 272 Antisense
AGCTCCAGAGGAAGTGCCAACCGGG 376 81 287 Antisense
CCAACCGGGGCAGCACACTAAATCG 521 273 303 Antisense
Sequence Source Affymetrix Proprietary Database