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[183125_at]

Description F25E5.8 /REP_DB=WormBase Gene ID /WP=CE07142 /TR=O76647 /GB=AAG24071.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 04      4grp_high_8_8 > 38      6grp_highest_nonorm > 26      6grp_total > 30      4grp_total > 33      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2805
1 2 3 4 range
A 2546.2
(-3)
P 		5344.1
(3)
P 		4587.7
(1.4)
P 		4878.4
(2)
P 		2798
B 2539.5
(-3)
P 		5261.4
(3)
P 		4783
(1.9)
P 		5105.2
(2.7)
P 		2722
C 2764.1
(-3)
P 		4886.5
(3)
P 		4472.8
(1.8)
P 		3909
(0.2)
P 		2122
avr 2616.6
(-3) 		5164
(3) 		4614.5
(1.7) 		4630.9
(1.7) 		2547

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4562
1 2 3 4 5 6 range
A 4345.9
(-0.5)
P 		5748.9
(1.4)
P 		6998.8
(3)
P 		5899.6
(1.6)
P 		4375.1
(-0.5)
P 		2436.9
(-3)
P 		4562
B 3830.8
(-1.2)
P 		5553.3
(1.2)
P 		6814.9
(3)
P 		5262
(0.8)
P 		4899.3
(0.3)
P 		2527.8
(-3)
P 		4287
C 3090.2
(-2.9)
P 		5818
(1.7)
P 		6442
(2.7)
P 		6614.2
(3)
P 		4282.6
(-0.9)
P 		3032.1
(-3)
P 		3582
avr 3755.6
(-1.4) 		5706.7
(1.5) 		6751.9
(3) 		5925.3
(1.8) 		4519
(-0.3) 		2665.6
(-3) 		4086
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 900
1 2 3 4 range
A 927.1
(-0.4)
P 		1160.1
(2.5)
P 		1199.3
(3)
P 		714.1
(-3)
P 		485
B 809.8
(-3)
P 		1299.4
(3)
P 		1108.3
(0.7)
P 		1087.9
(0.4)
P 		490
C 915
(-1.3)
P 		1404.9
(2)
P 		1558.9
(3)
P 		659.1
(-3)
P 		900
avr 884
(-2.2) 		1288.1
(3) 		1288.8
(3) 		820.4
(-3) 		468

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1451
1 2 3 4 5 6 range
A 801.1
(-2.1)
P 		2044.6
(3)
P 		1524.2
(0.8)
P 		1496.3
(0.7)
P 		1320.2
(0)
P 		593.3
(-3)
P 		1451
B 1332.9
(-0.8)
P 		1848.4
(2.2)
P 		1654.9
(1.1)
P 		1393.7
(-0.4)
P 		1990.5
(3)
P 		942.6
(-3)
P 		1048
C 1145.4
(-2)
P 		1775.2
(3)
P 		1639.6
(1.9)
P 		1330.1
(-0.5)
P 		1385.8
(-0.1)
P 		1015
(-3)
P 		760
avr 1093.1
(-1.6) 		1889.4
(3) 		1606.2
(1.4) 		1406.7
(0.2) 		1565.5
(1.1) 		850.3
(-3) 		1039
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183125_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12383
Sequence Type Exemplar sequence
Representative Public ID F25E5.8 NCBI
Target Description F25E5.8 /REP_DB=WormBase Gene ID /WP=CE07142 /TR=O76647 /GB=AAG24071.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072508(11), NM_182395(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072508 NCBI Caenorhabditis elegans F25E5.8a (F25E5.8) mRNA, complete cds. 11/11 None
NM_182395 NCBI Caenorhabditis elegans F25E5.8b (F25E5.8) mRNA, complete cds. 11/11 None
SNAP00000010674 ENSEMBL cdna:SNAP chromosome:CEL140:V:7438534:7439496:1 11/11 None
GENEFINDER00000010690 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7438534:7439496:1 11/11 None
F25E5.8a.2 ENSEMBL cdna:known chromosome:CEL140:V:7438520:7439905:1 gene:F25E5.8 11/11 None
F25E5.8a.1 ENSEMBL cdna:known chromosome:CEL140:V:7438522:7439905:1 gene:F25E5.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F25E5.8b 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7438534-7439497(+) 100.0 100.0
Public Domain and Genome References
Ensembl F25E5.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG24071 Hypothetical protein F25E5.8a [Caenorhabditis elegans] ref|NP_504909.1| F25E5.8a [Caenorhabditis elegans] 1.0E-90
blast CAE64149 Hypothetical protein CBG08766 [Caenorhabditis briggsae] 1.0E-60
Sequence
Target Sequence 
>C. ELEGANS:183125_AT
aaagcatttgccaccggttgttgaaaacgacggcgacacacattccaatcacagtgatgt
gtccgggtcgggatcctcattaaaagaaaagaagcatcacggattgactgctcatttcaa
agatctatttcatattgggcaccaccatcatcatcacggagagcatccacactcgaagca
aaattctggagcttctacgccggcaaggaaaaactcgactggaagtgaatgtccgtgctc
gaagcaagaacaatgtcacaagcattcgattaatatcactgttggagcggacgaagttcc
aacggttcttgcgactccaccaaacgaacaaaatatccttcatgctcattttatcaaaaa
ccaatcgccatgcatatcacgagtagtcagctcaagctcactgaaagaaaaacttcataa
aatcaaaagaactaccagttccgacagcgacactgatttccctgagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCATTTGCCACCGGTTGTTGAA 683 123 213 Antisense
AGTGATGTGTCCGGGTCGGGATCCT 662 59 265 Antisense
GCATCACGGATTGACTGCTCATTTC 459 309 306 Antisense
TCATATTGGGCACCACCATCATCAT 165 621 342 Antisense
TCATCACGGAGAGCATCCACACTCG 164 621 363 Antisense
CAAAATTCTGGAGCTTCTACGCCGG 339 189 391 Antisense
GACGAAGTTCCAACGGTTCTTGCGA 349 377 502 Antisense
GTTCTTGCGACTCCACCAAACGAAC 298 443 517 Antisense
AAAACCAATCGCCATGCATATCACG 365 131 569 Antisense
AGAACTACCAGTTCCGACAGCGACA 71 73 640 Antisense
ACAGCGACACTGATTTCCCTGAGAA 480 107 656 Antisense
Sequence Source Affymetrix Proprietary Database