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[183110_at]

Description F25B4.8 /REP_DB=WormBase Gene ID /WP=CE09626 /TR=Q22965 /GB=AAB37079.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 72      6grp_total > 63      4grp_total > 0      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 397
1 2 3 4 range
A 445.7
(-3)
P 		458.8
(-2.2)
P 		445.4
(-3)
P 		547.3
(3)
P 		102
B 425.4
(-0.8)
P 		282.4
(-3)
P 		487.3
(0.1)
P 		679.4
(3)
P 		397
C 512.9
(0)
P 		365.3
(-3)
P 		569.8
(1.2)
P 		656.3
(3)
P 		291
avr 461.3
(-0.9) 		368.8
(-3) 		500.8
(0.1) 		627.7
(3) 		259

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 319
1 2 3 4 5 6 range
A 305
(-2.7)
P 		348
(-1.7)
P 		558.5
(3)
P 		453.2
(0.7)
P 		325.4
(-2.2)
P 		289.4
(-3)
P 		269
B 315.1
(-3)
P 		430.3
(-0.6)
P 		608.7
(3)
P 		469
(0.1)
P 		457.5
(-0.1)
P 		356
(-2.2)
P 		294
C 328.3
(-3)
P 		402.9
(0.9)
P 		443.5
(3)
P 		417.2
(1.6)
P 		352.7
(-1.7)
P 		440.4
(2.8)
P 		115
avr 316.1
(-3) 		393.7
(-0.9) 		536.9
(3) 		446.5
(0.5) 		378.5
(-1.3) 		361.9
(-1.8) 		221
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 162.3
(3)
P 		99.6
(-1.6)
P 		116.4
(-0.4)
P 		80.1
(-3)
P 		82
B 135.6
(2.3)
P 		69.7
(-3)
P 		112.9
(0.5)
P 		144.8
(3)
P 		75
C 169.8
(1.2)
P 		105
(-3)
P 		198.6
(3)
P 		110.7
(-2.6)
P 		94
avr 155.9
(3) 		91.4
(-3) 		142.6
(1.8) 		111.9
(-1.1) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 56.2
(-3)
P 		123.8
(3)
P 		121.6
(2.8)
P 		114.9
(2.2)
P 		98.2
(0.7)
P 		70.5
(-1.7)
P 		68
B 109.7
(-3)
P 		143.2
(-0.4)
P 		147.8
(0)
P 		124.2
(-1.9)
P 		185.9
(3)
P 		132.8
(-1.2)
P 		76
C 121.7
(0.6)
P 		123
(0.7)
P 		112.9
(-0.3)
P 		83.9
(-3)
P 		114.1
(-0.1)
P 		147.4
(3)
P 		64
avr 95.9
(-3) 		130
(2.6) 		127.4
(2.1) 		107.7
(-1.1) 		132.7
(3) 		116.9
(0.4) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183110_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11873
Sequence Type Exemplar sequence
Representative Public ID F25B4.8 NCBI
Target Description F25B4.8 /REP_DB=WormBase Gene ID /WP=CE09626 /TR=Q22965 /GB=AAB37079.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171460(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171460 NCBI Caenorhabditis elegans F25B4.8b (F25B4.8) mRNA, complete cds. 10/11 None
GENEFINDER00000033039 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5692746:5694151:-1 11/11 None
F25B4.8b ENSEMBL cdna:known chromosome:CEL140:V:5692725:5694310:-1 gene:F25B4.8 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171459 7/11 Cross Hyb Matching Probes None
F25B4.8a 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
184918 2 1 NM_171460 183110_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5692745-5694151(-) 88.1 100.0
Public Domain and Genome References
Ensembl F25B4.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T25729 hypothetical protein F25B4.8 - Caenorhabditis elegans 2.0E-69
blast AAM29679 Hypothetical protein F25B4.8b [Caenorhabditis elegans] ref|NP_741541.1| F25B4.8b [Caenorhabditis elegans] 3.0E-66
blast AAM29678 Hypothetical protein F25B4.8a [Caenorhabditis elegans] ref|NP_741540.1| F25B4.8a [Caenorhabditis elegans] 2.0E-52
blast CAE64586 Hypothetical protein CBG09341 [Caenorhabditis briggsae] 2.0E-50
blast AAM29678 Hypothetical protein F25B4.8a [Caenorhabditis elegans] ref|NP_741540.1| F25B4.8a [Caenorhabditis elegans] 4.0E-32
blast CAE64586 Hypothetical protein CBG09341 [Caenorhabditis briggsae] 4.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR006913 EMBL-EBI Protein of unknown function DUF636 2.2E-22
Sequence
Target Sequence 
>C. ELEGANS:183110_AT
tggaagttgtcattgtggggcggtgaagtggagttttacaggaccagaggttctcgatgg
ttgcagatgcaaggagaggataaaatcacagtctatcgttttaacactgggcttgctcaa
cacaagttctgttcgatttgtggggttcaaagcttctatcatccgcggtctaacccagac
gcagttggtaagccagaatccacgtggtgtcaggccgtcccgtcgtggcttgatctacca
aaaatcaatcatgcctcactgtatcgacggaaagaccctcaaaggtgtcaactggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAAGTTGTCATTGTGGGGCGGTG 96 547 33 Antisense
GAGGTTCTCGATGGTTGCAGATGCA 461 403 79 Antisense
AAATCACAGTCTATCGTTTTAACAC 443 117 115 Antisense
TTAACACTGGGCTTGCTCAACACAA 506 683 133 Antisense
AGTTCTGTTCGATTTGTGGGGTTCA 38 63 157 Antisense
GGGTTCAAAGCTTCTATCATCCGCG 130 497 175 Antisense
CGGTCTAACCCAGACGCAGTTGGTA 547 199 198 Antisense
GGTAAGCCAGAATCCACGTGGTGTC 54 505 219 Antisense
CCGTCGTGGCTTGATCTACCAAAAA 149 265 252 Antisense
ATCAATCATGCCTCACTGTATCGAC 231 27 276 Antisense
AGACCCTCAAAGGTGTCAACTGGAC 120 69 305 Antisense
Sequence Source Affymetrix Proprietary Database