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[183106_at]

Description F32B5.3 /REP_DB=WormBase Gene ID /WP=CE09851 /TR=O01852 /GB=AAB54211.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 47.9
(1.5)
A 		56.6
(3)
A 		21
(-3)
A 		46
(1.2)
A 		36
B 28.9
(2.1)
A 		16.7
(-0.7)
A 		6.6
(-3)
A 		33
(3)
A 		26
C 67.9
(3)
A 		35.9
(-0.7)
A 		15.8
(-3)
A 		53.5
(1.3)
A 		52
avr 48.2
(3) 		36.4
(0.9) 		14.5
(-3) 		44.2
(2.3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 62.5
(3)
A 		28.4
(-0.8)
A 		60.7
(2.8)
P 		27.9
(-0.9)
A 		14.9
(-2.3)
A 		8.8
(-3)
A 		54
B 19.7
(-1.1)
A 		23.6
(-0.5)
A 		9.2
(-2.5)
A 		29.1
(0.2)
A 		5.6
(-3)
A 		49.3
(3)
A 		44
C 41.7
(3)
A 		12.9
(-3)
A 		40.1
(2.7)
A 		15.2
(-2.5)
A 		28.3
(0.2)
A 		28.5
(0.3)
A 		29
avr 41.3
(3) 		21.6
(-1.7) 		36.7
(1.9) 		24.1
(-1.1) 		16.3
(-3) 		28.9
(0) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 17.4
(3)
A 		12.3
(0.4)
A 		5.5
(-3)
A 		6.7
(-2.4)
A 		12
B 9.2
(3)
A 		4.1
(-1)
A 		1.5
(-3)
A 		7
(1.3)
A 		8
C 22.5
(3)
A 		10.3
(-1.3)
A 		5.5
(-3)
A 		9
(-1.8)
A 		17
avr 16.4
(3) 		8.9
(-0.7) 		4.2
(-3) 		7.6
(-1.3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 11.5
(2.1)
A 		10.1
(1.3)
A 		13.2
(3)
P 		7.1
(-0.3)
A 		4.5
(-1.7)
A 		2.1
(-3)
A 		11
B 6.9
(-1.3)
A 		7.9
(-0.9)
A 		2.2
(-3)
A 		7.7
(-1)
A 		2.3
(-3)
A 		18.4
(3)
A 		16
C 15.5
(3)
A 		3.9
(-2.6)
A 		10.2
(0.4)
A 		3.1
(-3)
A 		9.2
(-0)
A 		9.5
(0.1)
A 		12
avr 11.3
(3) 		7.3
(-1) 		8.5
(0.2) 		6
(-2.3) 		5.3
(-3) 		10
(1.7) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183106_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.173
Sequence Type Exemplar sequence
Representative Public ID F32B5.3 NCBI
Target Description F32B5.3 /REP_DB=WormBase Gene ID /WP=CE09851 /TR=O01852 /GB=AAB54211.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058624(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058624 NCBI Caenorhabditis elegans F32B5.3 (F32B5.3) mRNA, complete cds. 11/11 None
SNAP00000012561 ENSEMBL cdna:SNAP chromosome:CEL140:I:2692500:2694085:1 11/11 None
GENEFINDER00000012567 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2692758:2694085:1 11/11 None
F32B5.3 ENSEMBL cdna:known chromosome:CEL140:I:2693103:2694085:1 gene:F32B5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000029536 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2693102-2694085(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F32B5.3
Entrez Gene 185197 Entrez gene
SwissProt O01852 EMBL-EBI
WormBase CE09851 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54211 Hypothetical protein F32B5.3 [Caenorhabditis elegans] ref|NP_491025.1| F32B5.3 [Caenorhabditis elegans] 1.0E-141
blast AAB54211 Hypothetical protein F32B5.3 [Caenorhabditis elegans] ref|NP_491025.1| F32B5.3 [Caenorhabditis elegans] 1.0E-132
blast AAC48047 Hypothetical protein K04A8.1 [Caenorhabditis elegans] ref|NP_504669.1| K04A8.1 [Caenorhabditis elegans] 1.0E-113
blast CAE71910 Hypothetical protein CBG18972 [Caenorhabditis briggsae] 1.0E-111
blast AAC48047 Hypothetical protein K04A8.1 [Caenorhabditis elegans] ref|NP_504669.1| K04A8.1 [Caenorhabditis elegans] 1.0E-111
blast CAE71910 Hypothetical protein CBG18972 [Caenorhabditis briggsae] 1.0E-106
blast AAC48047 Hypothetical protein K04A8.1 [Caenorhabditis elegans] ref|NP_504669.1| K04A8.1 [Caenorhabditis elegans] 1.0E-106
Protein Domains
Database ID Description E-Value
scop a.4.1.DNA-binding domain of human telomeric protein, hTRF1 All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; DNA-binding domain of human telomeric protein, hTRF1 6.0
Pfam IPR004951 EMBL-EBI Caenorhabditis protein of unknown function DUF268 2.3E-126
Pfam IPR004951 EMBL-EBI Caenorhabditis protein of unknown function DUF268 1.0E-126
Pfam IPR004951 EMBL-EBI Caenorhabditis protein of unknown function DUF268 2.3E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012567 2 7-24,95-117
Sequence
Target Sequence 
>C. ELEGANS:183106_AT
acaactgggtggtctagcatatggtaaagatggagtatccatgtttgatgctatgcaagc
tcacagactagataacatgagcggagttgtgattggaagtatgcagccttgggttgaagt
gtctgctcttcggagtggtgtatcaaaggtgctgacagttgaatataacaatctaaccat
tcaagaagagttcaagaaccggatatacgccggaaaatttgattttgccgcttccttctc
atctatcgagcattctggattaggtagatacggtgacccaatggatcctatcggtgatat
cagggagatgctgaaaatcaagtgtgtcttgaagccaggaggtctgctgtttatcgggtt
cccattgggcactgatgctattcaatttaatgctcatagaatctacggctcaatccgtct
tgcaatgatgatgtatggattcgaatggatcgatacattctctggagactctgaacaacc
aaatgatctcacatctgagcggcttcatgcagcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAACTGGGTGGTCTAGCATATGGT 261 113 243 Antisense
GATGCTATGCAAGCTCACAGACTAG 593 413 289 Antisense
GGGTTGAAGTGTCTGCTCTTCGGAG 676 495 353 Antisense
GAAAATTTGATTTTGCCGCTTCCTT 340 353 455 Antisense
CTTCCTTCTCATCTATCGAGCATTC 501 219 473 Antisense
ACGGTGACCCAATGGATCCTATCGG 622 95 512 Antisense
GGAGGTCTGCTGTTTATCGGGTTCC 32 517 580 Antisense
ATCGGGTTCCCATTGGGCACTGATG 446 35 595 Antisense
GAATCTACGGCTCAATCCGTCTTGC 706 325 641 Antisense
GGATCGATACATTCTCTGGAGACTC 78 513 689 Antisense
ACATCTGAGCGGCTTCATGCAGCAC 218 107 733 Antisense
Sequence Source Affymetrix Proprietary Database