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[183104_at]

Description F56A12.2 /REP_DB=WormBase Gene ID /WP=CE11224 /TR=O45571 /GB=CAB04482.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 13      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 235
1 2 3 4 range
A 179.8
(2.5)
A 		113.1
(-1.6)
A 		91.2
(-3)
A 		187.7
(3)
P 		97
B 162.8
(-0.2)
P 		107.2
(-1.8)
A 		64.7
(-3)
A 		277.4
(3)
P 		213
C 184.2
(0.8)
P 		82.8
(-1.9)
A 		42.5
(-3)
A 		267.7
(3)
P 		225
avr 175.6
(0.7) 		101
(-1.8) 		66.1
(-3) 		244.3
(3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 181.1
(2.8)
A 		172.2
(2.2)
P 		185
(3)
P 		95.1
(-2.5)
A 		153.8
(1.1)
A 		86.6
(-3)
A 		98
B 107.8
(-3)
A 		214.3
(3)
P 		138.3
(-1.3)
A 		159
(-0.1)
M 		155.3
(-0.3)
P 		115.5
(-2.6)
P 		107
C 99.9
(-2.4)
A 		202.6
(2.4)
A 		158.5
(0.3)
P 		215.3
(3)
M 		92.9
(-2.7)
A 		86.9
(-3)
M 		128
avr 129.6
(-1) 		196.4
(3) 		160.6
(0.9) 		156.5
(0.6) 		134
(-0.7) 		96.3
(-3) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 65.5
(3)
A 		24.6
(-2.9)
A 		23.8
(-3)
A 		27.5
(-2.5)
P 		42
B 51.9
(2)
P 		26.5
(-1.4)
A 		15
(-3)
A 		59.1
(3)
P 		44
C 61
(3)
P 		23.8
(-1.8)
A 		14.8
(-3)
A 		45.1
(0.9)
P 		46
avr 59.5
(3) 		25
(-2) 		17.9
(-3) 		43.9
(0.8) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 33.4
(-1.2)
A 		61.2
(3)
P 		40.3
(-0.1)
P 		24.1
(-2.6)
A 		46.4
(0.8)
A 		21.1
(-3)
A 		40
B 37.5
(-2.4)
A 		71.3
(3)
P 		33.6
(-3)
A 		42.1
(-1.6)
M 		63.1
(1.7)
P 		43.1
(-1.5)
P 		38
C 37
(-1.6)
A 		61.8
(3)
A 		40.3
(-0.9)
P 		43.3
(-0.4)
M 		30.1
(-2.8)
A 		29.1
(-3)
M 		33
avr 36
(-2.1) 		64.8
(3) 		38.1
(-1.8) 		36.5
(-2) 		46.5
(-0.3) 		31.1
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183104_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12989
Sequence Type Exemplar sequence
Representative Public ID F56A12.2 NCBI
Target Description F56A12.2 /REP_DB=WormBase Gene ID /WP=CE11224 /TR=O45571 /GB=CAB04482.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074163(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074163 NCBI Caenorhabditis elegans F56A12.2 (F56A12.2) mRNA, complete cds. 11/11 None
SNAP00000028032 ENSEMBL cdna:SNAP chromosome:CEL140:V:14378727:14381654:-1 11/11 None
GENEFINDER00000028033 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14378686:14381667:-1 11/11 None
F56A12.2 ENSEMBL cdna:known chromosome:CEL140:V:14378687:14381654:-1 gene:F56A12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14378726-14381654(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F56A12.2
Entrez Gene 179937 Entrez gene
SwissProt O45571 EMBL-EBI
WormBase CE37975 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04482 Hypothetical protein F56A12.2 [Caenorhabditis elegans] ref|NP_506564.2| F56A12.2 [Caenorhabditis elegans] 0.0
blast CAE60910 Hypothetical protein CBG04627 [Caenorhabditis briggsae] 0.0
blast CAE60910 Hypothetical protein CBG04627 [Caenorhabditis briggsae] 1.0E-172
blast CAE58477 Hypothetical protein CBG01619 [Caenorhabditis briggsae] 5.0E-76
blast CAE58477 Hypothetical protein CBG01619 [Caenorhabditis briggsae] 1.0E-75
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506564.2 5 36-58,65-87,107-129,201-223,260-282
F56A12.2 5 36-58,65-87,107-129,201-223,260-282
SNAP00000028032 5 36-58,65-87,107-129,201-223,260-282
GENEFINDER00000028033 5 41-63,70-92,112-134,206-228,265-287
Sequence
Target Sequence 
>C. ELEGANS:183104_AT
gttccaatccttctggtaatcctctgcttcgtcattcatagcacttcctactttgaattc
aaccttattacatgctttgatgaagttcatcagactgaatcaaaaatgctccagatgaca
caactcagaggagattcttattggtattttcaattcaaagttgcccttaccatgacaaca
gagacattgggaccaatgcttttcatctccacgctgtcattgttcactgaatacaaaatg
catcagaatgtcaaggaacggaggaggctttttgagagtcagaagagaagtagaaacaca
ctggtgacggaggaattgaaggataaagcatcgaaggcgttagctgtgttcattgttgtc
aaatttctgattttgagatccctgccaacattgattgatctctacgaggttctcattgaa
aacagtataaacttcggcccattcatgacaaaagtcacgcgaatctcagacttccttgtc
attctcaactcggcaaccaacacattggcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCCAATCCTTCTGGTAATCCTCT 273 441 634 Antisense
ATCCTCTGCTTCGTCATTCATAGCA 432 39 652 Antisense
GCACTTCCTACTTTGAATTCAACCT 580 315 674 Antisense
ATTCAAAGTTGCCCTTACCATGACA 349 9 786 Antisense
TCCACGCTGTCATTGTTCACTGAAT 219 589 841 Antisense
GGCGTTAGCTGTGTTCATTGTTGTC 483 541 969 Antisense
GAGATCCCTGCCAACATTGATTGAT 176 397 1008 Antisense
GTATAAACTTCGGCCCATTCATGAC 356 451 1058 Antisense
AGTCACGCGAATCTCAGACTTCCTT 173 61 1086 Antisense
TCAGACTTCCTTGTCATTCTCAACT 51 625 1099 Antisense
AACTCGGCAACCAACACATTGGCCT 464 141 1120 Antisense
Sequence Source Affymetrix Proprietary Database