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[183103_at]

Description F25E5.2 /REP_DB=WormBase Gene ID /WP=CE07136 /TR=O76655 /GB=AAG24073.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 42      4grp_high_8_8 > 55      6grp_total > 20      4grp_total > 48      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 84.8
(-3)
M 		274.3
(3)
P 		264.7
(2.7)
P 		130.9
(-1.5)
A 		190
B 115.9
(-2.4)
P 		220.4
(0.4)
P 		321.1
(3)
P 		92.1
(-3)
A 		229
C 102.3
(-3)
A 		168.9
(-0.9)
P 		292.4
(3)
P 		121.6
(-2.4)
P 		190
avr 101
(-3) 		221.2
(0.8) 		292.7
(3) 		114.9
(-2.6) 		192

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 100.5
(-2.1)
A 		284.8
(2.3)
P 		314.8
(3)
P 		264.4
(1.8)
P 		166.5
(-0.5)
P 		60.6
(-3)
A 		254
B 75.3
(-3)
P 		307.1
(3)
P 		217.1
(0.7)
P 		196.5
(0.1)
P 		232.2
(1.1)
P 		76.6
(-3)
P 		232
C 85
(-2.4)
A 		229.8
(1.9)
P 		202.6
(1.1)
P 		268.1
(3)
P 		148.2
(-0.6)
P 		65.7
(-3)
P 		202
avr 86.9
(-2.4) 		273.9
(3) 		244.8
(2.2) 		243
(2.1) 		182.3
(0.3) 		67.6
(-3) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 30.9
(-1.6)
M 		59.5
(1.8)
P 		69.2
(3)
P 		19.2
(-3)
A 		50
B 37
(-1.1)
P 		54.4
(0.8)
P 		74.4
(3)
P 		19.6
(-3)
A 		55
C 33.9
(-2)
A 		48.6
(-0.9)
P 		101.9
(3)
P 		20.5
(-3)
P 		81
avr 33.9
(-1.6) 		54.2
(0.3) 		81.8
(3) 		19.8
(-3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 18.5
(-2.7)
A 		101.3
(3)
P 		68.6
(0.7)
P 		67.1
(0.6)
P 		50.2
(-0.5)
P 		14.7
(-3)
A 		87
B 26.2
(-3)
P 		102.2
(3)
P 		52.7
(-0.9)
P 		52
(-1)
P 		94.3
(2.4)
P 		28.6
(-2.8)
P 		76
C 31.5
(-1.8)
A 		70.1
(3)
P 		51.6
(0.7)
P 		53.9
(1)
P 		48
(0.2)
P 		22
(-3)
P 		48
avr 25.4
(-2.7) 		91.2
(3) 		57.6
(0.1) 		57.7
(0.1) 		64.2
(0.7) 		21.8
(-3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183103_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12343
Sequence Type Exemplar sequence
Representative Public ID F25E5.2 NCBI
Target Description F25E5.2 /REP_DB=WormBase Gene ID /WP=CE07136 /TR=O76655 /GB=AAG24073.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072516(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072516 NCBI Caenorhabditis elegans F25E5.2 (F25E5.2) mRNA, complete cds. 11/11 None
SNAP00000010681 ENSEMBL cdna:SNAP chromosome:CEL140:V:7458419:7460042:1 11/11 None
GENEFINDER00000010691 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7458419:7460413:1 11/11 None
F25E5.2 ENSEMBL cdna:known chromosome:CEL140:V:7458419:7460042:1 gene:F25E5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7458419-7460043(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.2
Entrez Gene 184924 Entrez gene
SwissProt O76655 EMBL-EBI
WormBase CE07136 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG24073 Hypothetical protein F25E5.2 [Caenorhabditis elegans] ref|NP_504917.1| F25E5.2 [Caenorhabditis elegans] 0.0
blast CAE73390 Hypothetical protein CBG20830 [Caenorhabditis briggsae] 1.0E-101
blast CAB04045 Hypothetical protein F01D5.10 [Caenorhabditis elegans] ref|NP_496940.1| F01D5.10 [Caenorhabditis elegans] 3.0E-92
blast CAB04045 Hypothetical protein F01D5.10 [Caenorhabditis elegans] ref|NP_496940.1| F01D5.10 [Caenorhabditis elegans] 1.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR007669 EMBL-EBI Protein of unknown function DUF595 1.5E-20
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504917.1 1 37-56
F25E5.2 1 37-56
SNAP00000010681 1 37-56
GENEFINDER00000010691 1 37-56
Sequence
Target Sequence 
>C. ELEGANS:183103_AT
aatccttcgctattcacagcggcgaacaagacaatttcacaggatttggtagagaatggt
ttgtgctcaaaagaagattcattccgaatgactacatgggataaatggacaaaatacgtc
actgacaaagtttgccatgtggcaatcattcaacaccctcttgaacggttttcccgatct
tttgttgaaatatgcataaaacaaaaagcatgctataactgtcaggacaacgtagattgc
tttatgaaaaaattgctgagagagcattcgccacttgcagaagggttggagggaaattat
agaacatatgtgacacatcatttcagcccacaaacctggaattgtcatttcaaccgcgac
ttaaataagattcaatttctgaagtttggaggaagcaaggaagatcttgccaaatttcta
accgatttggataaaattctaggagaacaaggcgttgaggcagatgttcgaagtgttatt
accaatgagataaataagatcccgttggaattaactcctgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCCTTCGCTATTCACAGCGGCGA 10 169 406 Antisense
ATGTGGCAATCATTCAACACCCTCT 406 49 542 Antisense
AAAGCATGCTATAACTGTCAGGACA 140 123 610 Antisense
TGCTGAGAGAGCATTCGCCACTTGC 487 581 659 Antisense
GCATTCGCCACTTGCAGAAGGGTTG 272 307 669 Antisense
TATGTGACACATCATTTCAGCCCAC 504 655 712 Antisense
TTCAGCCCACAAACCTGGAATTGTC 689 687 727 Antisense
TTGTCATTTCAACCGCGACTTAAAT 307 705 747 Antisense
GAAGATCTTGCCAAATTTCTAACCG 499 333 805 Antisense
AGGCGTTGAGGCAGATGTTCGAAGT 346 53 855 Antisense
GATCCCGTTGGAATTAACTCCTGAG 525 413 903 Antisense
Sequence Source Affymetrix Proprietary Database