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[183091_at]

Description R04B3.1 /REP_DB=WormBase Gene ID /WP=CE07411 /TR=Q21696 /GB=AAA91259.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 35      4grp_total > 58      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 19.9
(-1.8)
A 		23.2
(-1.5)
A 		65
(3)
A 		9.2
(-3)
A 		56
B 28.4
(-0.1)
A 		52.5
(3)
A 		22.5
(-0.9)
A 		6.2
(-3)
A 		46
C 37.5
(-0.4)
A 		51.2
(1.3)
A 		65.5
(3)
A 		15.4
(-3)
A 		50
avr 28.6
(-0.3) 		42.3
(1.7) 		51
(3) 		10.3
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 18.8
(-1.6)
A 		65.6
(3)
A 		58.1
(2.3)
A 		5.2
(-3)
A 		8.7
(-2.7)
A 		48.1
(1.3)
A 		60
B 62
(3)
A 		40.1
(0.6)
A 		12.2
(-2.6)
A 		11.1
(-2.7)
A 		9.1
(-2.9)
A 		8.2
(-3)
A 		54
C 40.3
(1.4)
A 		44.5
(2)
A 		52.6
(3)
A 		5.7
(-3)
A 		10.5
(-2.4)
A 		33.5
(0.6)
A 		47
avr 40.4
(1.6) 		50.1
(3) 		41
(1.7) 		7.3
(-3) 		9.4
(-2.7) 		29.9
(0.2) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 7.2
(-0.7)
A 		5
(-1.6)
A 		17
(3)
A 		1.3
(-3)
A 		16
B 9.1
(1)
A 		13
(3)
A 		5.2
(-1)
A 		1.3
(-3)
A 		12
C 12.4
(-0.1)
A 		14.7
(0.6)
A 		22.8
(3)
A 		2.6
(-3)
A 		20
avr 9.6
(0.6) 		10.9
(1.2) 		15
(3) 		1.7
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 3.5
(-2.4)
A 		23.3
(3)
A 		12.7
(0.1)
A 		1.3
(-3)
A 		2.6
(-2.6)
A 		11.7
(-0.2)
A 		22
B 21.6
(3)
A 		13.3
(0.3)
A 		3
(-3)
A 		2.9
(-3)
A 		3.7
(-2.7)
A 		3.1
(-2.9)
A 		19
C 14.9
(3)
A 		13.6
(2.4)
A 		13.4
(2.3)
A 		1.1
(-3)
A 		3.4
(-2)
A 		11.2
(1.4)
A 		14
avr 13.3
(1.6) 		16.7
(3) 		9.7
(0.2) 		1.8
(-3) 		3.2
(-2.4) 		8.7
(-0.2) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183091_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18462
Sequence Type Exemplar sequence
Representative Public ID R04B3.1 NCBI
Target Description R04B3.1 /REP_DB=WormBase Gene ID /WP=CE07411 /TR=Q21696 /GB=AAA91259.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076046(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076046 NCBI Caenorhabditis elegans R04B3.1 (R04B3.1) mRNA, complete cds. 11/11 None
SNAP00000030419 ENSEMBL cdna:SNAP chromosome:CEL140:X:2467344:2473577:-1 9/11 None
GENEFINDER00000030424 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2467344:2473577:-1 11/11 None
R04B3.1 ENSEMBL cdna:known chromosome:CEL140:X:2467344:2470284:-1 gene:R04B3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2467343-2469672(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R04B3.1
Entrez Gene 187565 Entrez gene
SwissProt Q21696 EMBL-EBI
WormBase CE37659 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA91259 Hypothetical protein R04B3.1 [Caenorhabditis elegans] ref|NP_508447.2| R04B3.1 [Caenorhabditis elegans] 0.0
blast CAE58874 Hypothetical protein CBG02105 [Caenorhabditis briggsae] 0.0
blast AAA91260 Hypothetical protein R04B3.2 [Caenorhabditis elegans] ref|NP_508448.1| R04B3.2 [Caenorhabditis elegans] sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor (Glycosylasparaginase) (Aspartylglucosaminidase) (N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase) (AGA) [Contains: Glycosylasparaginase alpha chain; Glycosylasparaginase beta chain] 0.0
blast AAA91260 Hypothetical protein R04B3.2 [Caenorhabditis elegans] ref|NP_508448.1| R04B3.2 [Caenorhabditis elegans] sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor (Glycosylasparaginase) (Aspartylglucosaminidase) (N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase) (AGA) [Contains: Glycosylasparaginase alpha chain; Glycosylasparaginase beta chain] 1.0E-176
Protein Domains
Database ID Description E-Value
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 1.0E-115
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 5.2E-60
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:183091_AT
ccgacgcttgtcaacgcatcgagttcattaaatgagcttattcttctagataaaagcgca
gaaaatgattgccaatccctacaatgcttgatggatcaacaaaatgcaccatgtgattac
aaacgttctgggactggagcatctggtgaaggatacttagttggttggagaatcacatct
gctaaaaagaatgtggcaaataagctatcgggtatacatgcgtctcccgaaccgtccaaa
gattcttcattcttggtagctaattttatgggattcagtcgaacgaagagcgaagatcgg
tttgtcttggaagcgccggagttcacaattagtgacgcaccgggcgcctacctaagcttc
cgcaaatttctctcaacaaaaggattgtacctctccgtttgtgaagatggctatgcaact
aggtgcttctggaatgtatatgaaaacagtattaggccgtttgcaagagagtggacaaag
gaagtggtactgttaccgggacaattaactaccttctacatcgtggcatggcaaaatacc
tcaacagtgatgaatgttggacaagttggaataactgaaatcgggctcttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGACGCTTGTCAACGCATCGAGTT 669 263 481 Antisense
TTGCCAATCCCTACAATGCTTGATG 342 701 549 Antisense
AAGCTATCGGGTATACATGCGTCTC 491 149 682 Antisense
TCCCGAACCGTCCAAAGATTCTTCA 371 597 705 Antisense
TGTCTTGGAAGCGCCGGAGTTCACA 222 557 783 Antisense
TCACAATTAGTGACGCACCGGGCGC 202 627 803 Antisense
ACCTAAGCTTCCGCAAATTTCTCTC 279 1 830 Antisense
AAAGGATTGTACCTCTCCGTTTGTG 342 119 859 Antisense
GGGACAATTAACTACCTTCTACATC 252 495 978 Antisense
TACCTTCTACATCGTGGCATGGCAA 304 649 990 Antisense
GAATAACTGAAATCGGGCTCTTCTC 250 325 1049 Antisense
Sequence Source Affymetrix Proprietary Database