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[183069_at]

Description Y94A7B.4 /REP_DB=WormBase Gene ID /WP=CE19282 /TR=Q9XXB0 /GB=CAA19566.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 40      4grp_total > 26      6grp_cgc > 40      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 36.4
(-3)
A 		60
(-0.4)
A 		40.1
(-2.6)
A 		90.1
(3)
A 		54
B 12.9
(-3)
A 		37.6
(1)
A 		47
(2.5)
A 		50.4
(3)
A 		38
C 46.3
(-1.2)
A 		69.3
(3)
A 		36.3
(-3)
A 		66
(2.4)
A 		33
avr 31.9
(-3) 		55.6
(0.9) 		41.1
(-1.5) 		68.8
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 46.3
(3)
A 		43.7
(2.3)
A 		35.6
(0.3)
A 		26.6
(-2)
A 		33.5
(-0.2)
A 		22.6
(-3)
A 		24
B 24
(0.6)
A 		22.4
(0.4)
A 		3.8
(-2.9)
A 		37.5
(3)
A 		8.4
(-2.1)
A 		3.2
(-3)
A 		34
C 11.6
(-2)
A 		30
(1.1)
A 		41.4
(3)
A 		39.5
(2.7)
A 		5.6
(-3)
A 		17.4
(-1)
A 		36
avr 27.3
(0.9) 		32
(2.3) 		26.9
(0.7) 		34.5
(3) 		15.8
(-2.6) 		14.4
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 13.3
(3)
A 		13
(2.4)
A 		10.5
(-3)
A 		13.2
(2.8)
A 		3
B 4.1
(-3)
A 		9.3
(1.6)
A 		10.9
(3)
A 		10.7
(2.8)
A 		7
C 15.3
(-0.1)
A 		19.9
(3)
A 		12.6
(-2)
A 		11.1
(-3)
A 		9
avr 10.9
(-3) 		14.1
(3) 		11.3
(-2.3) 		11.7
(-1.5) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 8.5
(-1.2)
A 		15.5
(3)
A 		7.7
(-1.7)
A 		6.8
(-2.2)
A 		10.1
(-0.2)
A 		5.5
(-3)
A 		10
B 8.3
(1.9)
A 		7.4
(1.3)
A 		0.9
(-3)
A 		9.9
(3)
A 		3.4
(-1.3)
A 		1.2
(-2.8)
A 		9
C 4.3
(-1.3)
A 		9.2
(2.1)
A 		10.5
(3)
A 		7.9
(1.2)
A 		1.8
(-3)
A 		5.8
(-0.2)
A 		9
avr 7
(-0.4) 		10.7
(3) 		6.4
(-1) 		8.2
(0.7) 		5.1
(-2.2) 		4.2
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183069_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13439
Sequence Type Exemplar sequence
Representative Public ID Y94A7B.4 NCBI
Target Description Y94A7B.4 /REP_DB=WormBase Gene ID /WP=CE19282 /TR=Q9XXB0 /GB=CAA19566.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075096(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075096 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-296) (srh-296) mRNA, complete cds. 11/11 None
SNAP00000042693 ENSEMBL cdna:SNAP chromosome:CEL140:V:17810226:17815907:1 10/11 None
Y94A7B.4 ENSEMBL cdna:known chromosome:CEL140:V:17812929:17815370:1 gene:Y94A7B.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000042702 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17812928-17815370(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-296
Ensembl Y94A7B.4
Entrez Gene 190793 Entrez gene
SwissProt Q9XXB0 EMBL-EBI
WormBase CE19282 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA19566 Hypothetical protein Y94A7B.4 [Caenorhabditis elegans] ref|NP_507497.1| Serpentine Receptor, class H family member (srh-296) [Caenorhabditis elegans] 1.0E-178
blast CAA19566 Hypothetical protein Y94A7B.4 [Caenorhabditis elegans] ref|NP_507497.1| Serpentine Receptor, class H family member (srh-296) [Caenorhabditis elegans] 1.0E-159
blast AAK72055 Serpentine receptor, class h protein 297 [Caenorhabditis elegans] ref|NP_494484.3| Serpentine Receptor, class H family member (srh-297) [Caenorhabditis elegans] 1.0E-113
blast AAK72055 Serpentine receptor, class h protein 297 [Caenorhabditis elegans] ref|NP_494484.3| Serpentine Receptor, class H family member (srh-297) [Caenorhabditis elegans] 1.0E-108
blast CAB03507 Hypothetical protein ZK1037.8 [Caenorhabditis elegans] ref|NP_506801.1| Serpentine Receptor, class H family member (srh-295) [Caenorhabditis elegans] 6.0E-99
blast CAB03507 Hypothetical protein ZK1037.8 [Caenorhabditis elegans] ref|NP_506801.1| Serpentine Receptor, class H family member (srh-295) [Caenorhabditis elegans] 2.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.8E-36
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 8.6E-13
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507497.1 7 15-34,47-69,89-108,128-150,197-216,237-259,274-293
Y94A7B.4 7 15-34,47-69,89-108,128-150,197-216,237-259,274-293
SNAP00000042693 10 57-79,115-137,150-172,206-228,241-263,283-302,322-344,391-410,431-453,468-487
Sequence
Target Sequence 
>C. ELEGANS:183069_AT
gaaaatcgccccagacgatgttggcgatatcttcgttatccattccttattatcaactac
ctttttggaataacctactttattccaattttcttgaatgtacctagtgatcaggagaat
gccaggcgcattctattcaatatgtatccagatgcttgcgaatacgtctctgacaagaat
ctggtatttgtggtggatattggtgacattgcttggtcaaagatacgagaaaatgctctc
acatttttgcttctttcggagattatagtgttggcagtggtgcttagagtgaaaatgagt
cgggcattgaaaacagcgattagtggtgacacactacgaatgcagaagaaattcttacga
gcgttgaatatacagatcgcaattccgatactcttgttctttgcccccgccgcaactgga
atcctaacaagccagcagacgtccaatgagcaattggagcacaacttgtttgtcatcaca
acgtcattccatggagttctatccacaattcttatgatctatttgcaaaagtgttataga
gatgtatttatcgggctttttgggtgtcgtaagcctgatcttgctatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAATCGCCCCAGACGATGTTGGC 623 353 367 Antisense
GCGATATCTTCGTTATCCATTCCTT 674 289 390 Antisense
CCATTCCTTATTATCAACTACCTTT 336 267 406 Antisense
ATGCCAGGCGCATTCTATTCAATAT 281 41 485 Antisense
GATGCTTGCGAATACGTCTCTGACA 529 411 517 Antisense
AATGCTCTCACATTTTTGCTTCTTT 256 167 598 Antisense
GCAATTCCGATACTCTTGTTCTTTG 131 323 745 Antisense
AAGCCAGCAGACGTCCAATGAGCAA 363 149 795 Antisense
GGAGCACAACTTGTTTGTCATCACA 202 521 822 Antisense
GTCATCACAACGTCATTCCATGGAG 555 467 838 Antisense
GTGTCGTAAGCCTGATCTTGCTATC 115 487 930 Antisense
Sequence Source Affymetrix Proprietary Database