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[183065_at]

Description C04F5.8 /REP_DB=WormBase Gene ID /WP=CE19672 /TR=Q9GZD0 /GB=AAF98591.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 49      6grp_high1_10_10 > 94      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 64      6grp_total > 85      4grp_total > 59      6grp_highest_sub > 04_05_06      6grp_high1_sub > In      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 661.3
(-0.9)
P 		780.1
(3)
P 		704.3
(0.5)
P 		596.2
(-3)
P 		184
B 735.9
(3)
P 		677.6
(1)
P 		699.2
(1.7)
P 		561.4
(-3)
P 		175
C 593
(-2.5)
P 		664.3
(0.2)
P 		738.1
(3)
P 		579.8
(-3)
P 		158
avr 663.4
(0.8) 		707.3
(2.7) 		713.9
(3) 		579.1
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 578
1 2 3 4 5 6 range
A 635
(-1.6)
P 		549.2
(-3)
P 		657.6
(-1.2)
P 		918.4
(3)
P 		798.6
(1.1)
P 		853.3
(1.9)
P 		369
B 601.5
(-3)
P 		709.2
(-1.1)
P 		714.4
(-1)
P 		862.3
(1.6)
P 		776.5
(0.1)
P 		944.1
(3)
P 		343
C 523.3
(-3)
P 		654.8
(-1.6)
P 		704.2
(-1.1)
P 		1101.2
(3)
P 		722.8
(-0.9)
P 		1043.2
(2.4)
P 		578
avr 586.6
(-3) 		637.7
(-2.2) 		692.1
(-1.3) 		960.6
(3) 		766
(-0.1) 		946.9
(2.8) 		374
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 240.8
(3)
P 		169.3
(0.2)
P 		184.1
(0.8)
P 		87.3
(-3)
P 		154
B 234.7
(3)
P 		167.3
(-0.5)
P 		162
(-0.8)
P 		119.6
(-3)
P 		115
C 196.3
(0.7)
P 		191
(0.5)
P 		257.2
(3)
P 		97.8
(-3)
P 		159
avr 223.9
(3) 		175.9
(0.6) 		201.1
(1.9) 		101.6
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 235
1 2 3 4 5 6 range
A 117.1
(-3)
P 		195.3
(0.8)
P 		143.2
(-1.7)
P 		232.9
(2.6)
P 		241
(3)
P 		207.8
(1.4)
P 		124
B 209.3
(-1.8)
P 		236.1
(-0.9)
P 		173.5
(-3)
P 		228.4
(-1.2)
P 		315.5
(1.8)
P 		352.1
(3)
P 		179
C 194
(-2.5)
P 		199.8
(-2.3)
P 		179.2
(-3)
P 		221.4
(-1.5)
P 		233.9
(-1.1)
P 		349.2
(3)
P 		170
avr 173.5
(-2.6) 		210.4
(-1) 		165.3
(-3) 		227.6
(-0.3) 		263.5
(1.3) 		303
(3) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183065_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15535
Sequence Type Exemplar sequence
Representative Public ID C04F5.8 NCBI
Target Description C04F5.8 /REP_DB=WormBase Gene ID /WP=CE19672 /TR=Q9GZD0 /GB=AAF98591.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071969(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071969 NCBI Caenorhabditis elegans C04F5.8 (C04F5.8) mRNA, complete cds. 11/11 None
SNAP00000034407 ENSEMBL cdna:SNAP chromosome:CEL140:V:5111402:5118969:-1 11/11 None
GENEFINDER00000034409 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5111402:5113566:-1 11/11 None
C04F5.8 ENSEMBL cdna:known chromosome:CEL140:V:5111380:5113567:-1 gene:C04F5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5111401-5113377(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C04F5.8
Entrez Gene 178898 Entrez gene
SwissProt Q9GZD0 EMBL-EBI
WormBase CE19672 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597561_AT similar to C04F5.8 cfa
CANINE_2:CFAAFFX.20616.1.S1_AT similar to C04F5.8 cfa
CHICKEN:GGAAFFX.6309.1.S1_AT similar to RIKEN cDNA 2700097O09 gga
MU11KSUBA:C76722_RC_AT RIKEN cDNA 2700097O09 gene mm
MG-U74AV2:160980_AT RIKEN cDNA 2700097O09 gene mm
MOE430A:1428810_AT RIKEN cDNA 2700097O09 gene mm
MOUSE430A_2:1428810_AT RIKEN cDNA 2700097O09 gene mm
MOUSE430_2:1428810_AT RIKEN cDNA 2700097O09 gene mm
RG-U34B:RC_AI030723_AT Similar to RIKEN cDNA 2700097O09 (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5544 calcium-dependent phospholipid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF98599 Hypothetical protein C04F5.9 [Caenorhabditis elegans] ref|NP_504371.1| C04F5.9 [Caenorhabditis elegans] 0.0
blast AAF98591 Hypothetical protein C04F5.8 [Caenorhabditis elegans] ref|NP_504370.1| C04F5.8 [Caenorhabditis elegans] 1.0E-152
blast CAE64075 Hypothetical protein CBG08678 [Caenorhabditis briggsae] 1.0E-145
blast CAE64075 Hypothetical protein CBG08678 [Caenorhabditis briggsae] 1.0E-144
blast CAE64075 Hypothetical protein CBG08678 [Caenorhabditis briggsae] 1.0E-123
blast XP_850590 PREDICTED: similar to C04F5.8 isoform 1 [Canis familiaris] 2.0E-26
Sequence
Target Sequence 
>C. ELEGANS:183065_AT
gtacgtactgtccggtttgcagttcgagggatactgtacctctaaacttcattagtcaca
gtttgagccgtcttcaagtcaaattcctttttgatctacttatgccgccaacatgccaac
acgtactggacattgggagcagattaggagctattgtttatggagcatcgatttttacaa
acggaaatgtgagcacaatcggcgttgaaatggactcggactatgctgctctcgcgttac
ggactattcgtcaattcggattaaagaacattgaaatcctgcgcgacgatatccgccacc
tcgcccacgtcttcaaggataatcaattcatcgtgatgaacaatgtcttctcgttcttcc
tatcaaaaaccgaacaagaagagtgctgggagttcctacatactcatatgcgccctggaa
cagtactcgctcatcatccaccaatcgaaaaaatcacggaacacttggatctctcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACGTACTGTCCGGTTTGCAGTTC 13 451 224 Antisense
GGGATACTGTACCTCTAAACTTCAT 379 495 251 Antisense
TTCCTTTTTGATCTACTTATGCCGC 170 697 307 Antisense
GCCAACATGCCAACACGTACTGGAC 56 279 330 Antisense
GAAATGGACTCGGACTATGCTGCTC 421 361 430 Antisense
CTCTCGCGTTACGGACTATTCGTCA 567 231 452 Antisense
TGAAATCCTGCGCGACGATATCCGC 592 571 495 Antisense
TGAACAATGTCTTCTCGTTCTTCCT 625 573 560 Antisense
AGTTCCTACATACTCATATGCGCCC 231 65 614 Antisense
ATATGCGCCCTGGAACAGTACTCGC 433 21 629 Antisense
TCACGGAACACTTGGATCTCTCATT 268 625 677 Antisense
Sequence Source Affymetrix Proprietary Database