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[183062_at]

Description F16B4.4 /REP_DB=WormBase Gene ID /WP=CE17014 /TR=O44632 /GB=AAB94235.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 73      4grp_highest_8_8 > 13      4grp_high_8_8 > 30      6grp_highest_nonorm > 57      6grp_total > 74      4grp_total > 24      6grp_high1_sub > 03_04      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3488
1 2 3 4 range
A 510
(-3)
P 		1856.2
(-0.5)
P 		2484.2
(0.6)
P 		3791.8
(3)
P 		3282
B 471.2
(-3)
P 		1773.9
(-0.7)
P 		2405.2
(0.4)
P 		3926.2
(3)
P 		3455
C 438.6
(-3)
P 		1955.1
(-0.2)
P 		2803
(1.4)
P 		3685.2
(3)
P 		3247
avr 473.3
(-3) 		1861.7
(-0.5) 		2564.1
(0.8) 		3801.1
(3) 		3328

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1825
1 2 3 4 5 6 range
A 1602.6
(-3)
P 		1704
(-2.7)
P 		3300.6
(2.6)
P 		3427.4
(3)
P 		2152.8
(-1.2)
P 		2223.4
(-1)
P 		1825
B 2055.4
(-3)
P 		2054.5
(-3)
P 		3375.8
(3)
P 		2579.8
(-0.6)
P 		2971.8
(1.2)
P 		2970.3
(1.2)
P 		1321
C 1992.8
(-3)
P 		2093
(-2.6)
P 		2610.2
(-0.3)
P 		3058
(1.6)
P 		2349.3
(-1.5)
P 		3382.4
(3)
P 		1390
avr 1883.6
(-3) 		1950.5
(-2.7) 		3095.5
(3) 		3021.7
(2.6) 		2491.3
(0) 		2858.7
(1.8) 		1212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 832
1 2 3 4 range
A 185.7
(-3)
P 		402.9
(-0.2)
P 		649.4
(3)
P 		555
(1.8)
P 		464
B 150.3
(-3)
P 		438.1
(-0.5)
P 		557.3
(0.6)
P 		836.7
(3)
P 		686
C 145.2
(-3)
P 		562.1
(0)
P 		976.9
(3)
P 		621.4
(0.4)
P 		832
avr 160.4
(-3) 		467.7
(0.2) 		727.9
(3) 		671
(2.4) 		568

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 912
1 2 3 4 5 6 range
A 295.4
(-3)
P 		606
(0.2)
P 		718.8
(1.4)
P 		869.3
(3)
P 		649.6
(0.7)
P 		541.3
(-0.4)
P 		574
B 715.2
(-2.6)
P 		683.8
(-3)
P 		819.7
(-1.4)
P 		683.3
(-3)
P 		1207.4
(3)
P 		1107.6
(1.9)
P 		524
C 738.7
(-1.6)
P 		638.6
(-2.7)
P 		664.3
(-2.4)
P 		615
(-3)
P 		760.2
(-1.3)
P 		1132.3
(3)
P 		517
avr 583.1
(-3) 		642.8
(-2) 		734.3
(-0.4) 		722.5
(-0.6) 		872.4
(2) 		927.1
(3) 		344
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183062_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15608
Sequence Type Exemplar sequence
Representative Public ID F16B4.4 NCBI
Target Description F16B4.4 /REP_DB=WormBase Gene ID /WP=CE17014 /TR=O44632 /GB=AAB94235.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071050(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071050 NCBI Caenorhabditis elegans F16B4.4 (F16B4.4) mRNA, complete cds. 11/11 None
SNAP00000004018 ENSEMBL cdna:SNAP chromosome:CEL140:V:1593964:1594388:1 11/11 None
GENEFINDER00000004040 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1593964:1594388:1 11/11 None
F16B4.4.2 ENSEMBL cdna:known chromosome:CEL140:V:1593942:1594502:1 gene:F16B4.4 11/11 None
F16B4.4.1 ENSEMBL cdna:known chromosome:CEL140:V:1593942:1594502:1 gene:F16B4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1593963-1594388(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F16B4.4
Entrez Gene 178648 Entrez gene
SwissProt O44632 EMBL-EBI
WormBase CE17014 Wormbase
Sequence
Target Sequence 
>C. ELEGANS:183062_AT
tgaaggttttcgcagttcttctcgctattgccgctgttgttgttaatgcttatccaggag
gttacggtagacctgctggaggatatggaccaccatccggaggttacggagctccaggag
gatacaacaaccaaggaggtttcgacggaggatcccaatttggaggtgacggaggcttcc
aacaaggaggcggattccaacaaggaggacaattccaacaaggaggcttcaaccagggag
gtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGGTTTTCGCAGTTCTTCTCGC 315 573 14 Antisense
TCGCTATTGCCGCTGTTGTTGTTAA 593 597 35 Antisense
GTTGTTGTTAATGCTTATCCAGGAG 406 437 49 Antisense
GGTTACGGTAGACCTGCTGGAGGAT 447 505 73 Antisense
AGGATATGGACCACCATCCGGAGGT 164 57 93 Antisense
CATCCGGAGGTTACGGAGCTCCAGG 374 209 107 Antisense
GCTCCAGGAGGATACAACAACCAAG 642 297 124 Antisense
GGAGGTTTCGACGGAGGATCCCAAT 121 517 148 Antisense
GACGGAGGCTTCCAACAAGGAGGCG 402 375 181 Antisense
GGAGGCGGATTCCAACAAGGAGGAC 651 513 199 Antisense
CAAGGAGGCTTCAACCAGGGAGGTG 700 181 232 Antisense
Sequence Source Affymetrix Proprietary Database