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[183045_s_at]

Description W02H5.8 /REP_DB=WormBase Gene ID /WP=CE21240 /TR=Q9N5C4 /GB=AAF60376.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 76      6grp_total > 77      4grp_total > 02      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 719
1 2 3 4 range
A 42.7
(-2.7)
A 		36.9
(-2.8)
A 		11
(-3)
A 		723.6
(3)
P 		713
B 30.1
(-2.6)
A 		4.7
(-3)
A 		6.7
(-3)
A 		438.6
(3)
P 		434
C 35.2
(-2.7)
A 		7.1
(-3)
A 		6.1
(-3)
A 		608.4
(3)
P 		602
avr 36
(-2.7) 		16.2
(-2.9) 		7.9
(-3) 		590.2
(3) 		582

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 396
1 2 3 4 5 6 range
A 109.8
(-2.7)
P 		130.2
(-2.4)
P 		114.5
(-2.7)
P 		94
(-3)
P 		129.6
(-2.4)
P 		463.1
(3)
P 		369
B 67.4
(-3)
A 		78.8
(-2.7)
P 		150
(-1.1)
P 		78.6
(-2.7)
P 		117.2
(-1.9)
P 		332.2
(3)
P 		265
C 106.9
(-2.5)
P 		88.7
(-3)
M 		212
(0.4)
P 		120
(-2.1)
P 		150.6
(-1.3)
P 		306.8
(3)
P 		218
avr 94.7
(-3) 		99.2
(-2.9) 		158.8
(-1.6) 		97.5
(-2.9) 		132.5
(-2.2) 		367.4
(3) 		273
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 15.6
(-2.3)
A 		8
(-2.7)
A 		2.9
(-3)
A 		105.9
(3)
P 		103
B 9.6
(-2.5)
A 		1.2
(-3)
A 		1.6
(-3)
A 		93.5
(3)
P 		92
C 11.6
(-2.4)
A 		2
(-3)
A 		2.1
(-3)
A 		102.6
(3)
P 		101
avr 12.3
(-2.4) 		3.7
(-2.9) 		2.2
(-3) 		100.7
(3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 20.2
(-3)
P 		46.3
(-1.3)
P 		24.9
(-2.7)
P 		23.8
(-2.8)
P 		39.1
(-1.8)
P 		112.8
(3)
P 		93
B 23.4
(-2.8)
A 		26.2
(-2.7)
P 		36.4
(-2.1)
P 		20.8
(-3)
P 		47.6
(-1.4)
P 		123.9
(3)
P 		103
C 39.6
(-1.8)
P 		27.1
(-2.8)
M 		54
(-0.7)
P 		24.1
(-3)
P 		48.7
(-1.1)
P 		102.7
(3)
P 		79
avr 27.7
(-2.7) 		33.2
(-2.3) 		38.4
(-2) 		22.9
(-3) 		45.1
(-1.5) 		113.1
(3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183045_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15325
Sequence Type Exemplar sequence
Representative Public ID W02H5.8 NCBI
Target Description W02H5.8 /REP_DB=WormBase Gene ID /WP=CE21240 /TR=Q9N5C4 /GB=AAF60376.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071323(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071323 NCBI Caenorhabditis elegans W02H5.8 (W02H5.8) mRNA, complete cds. 11/11 None
SNAP00000022935 ENSEMBL cdna:SNAP chromosome:CEL140:V:2646992:2652470:-1 11/11 None
W02H5.8 ENSEMBL cdna:known chromosome:CEL140:V:2646894:2652484:-1 gene:W02H5.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000022936 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2646991-2652470(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02H5.8
Entrez Gene 178730 Entrez gene
SwissProt Q9N5C4 EMBL-EBI
WormBase CE21240 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257866_AT dihydroxyacetone kinase family protein at
CANINE_2:CFA.14548.1.S1_AT similar to W02H5.8 cfa
CANINE_2:CFAAFFX.24860.1.S1_AT similar to W02H5.8 cfa
CANINE_2:CFAAFFX.24865.1.S1_AT similar to W02H5.8 cfa
CANINE_2:CFAAFFX.24865.1.S1_S_AT similar to W02H5.8 cfa
CANINE_2:CFAAFFX.24873.1.S1_AT similar to W02H5.8 cfa
CANINE_2:CFAAFFX.24873.1.S1_S_AT similar to W02H5.8 cfa
CHICKEN:GGAAFFX.26453.1.S1_AT similar to DKFZP586B1621 protein gga
HU35KSUBB:RC_H20543_AT dihydroxyacetone kinase 2 homolog (yeast) hs
HG-U133A:218688_AT dihydroxyacetone kinase 2 homolog (yeast) hs
HG-U133_PLUS_2:218688_AT dihydroxyacetone kinase 2 homolog (yeast) hs
HG-U133A_2:218688_AT dihydroxyacetone kinase 2 homolog (yeast) hs
U133_X3P:G13027381_3P_AT dihydroxyacetone kinase 2 homolog (yeast) hs
HG-U95B:46570_AT dihydroxyacetone kinase 2 homolog (yeast) hs
MOUSE430A_2:1425300_AT dihydroxyacetone kinase 2 homolog (yeast) mm
MOUSE430_2:1425300_AT dihydroxyacetone kinase 2 homolog (yeast) mm
MOE430A:1425300_AT dihydroxyacetone kinase 2 homolog (yeast) mm
MG-U74CV2:136565_AT dihydroxyacetone kinase 2 homolog (yeast) mm
MOUSE430_2:1447244_AT CDNA sequence BC021917 (BC021917), mRNA mm
MOE430B:1447244_AT CDNA sequence BC021917 (BC021917), mRNA mm
YEAST_2:1776664_AT Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6071 glycerol metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4371 glycerone kinase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503724 W02H5.8 [Caenorhabditis elegans] gb|AAF60376.1| Hypothetical protein W02H5.8 [Caenorhabditis elegans] 0.0
blast CAE62528 Hypothetical protein CBG06637 [Caenorhabditis briggsae] 0.0
blast XP_692491 PREDICTED: similar to dihydroxyacetone kinase (5D126) [Danio rerio] 1.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR004006 EMBL-EBI Dak kinase domain 2.9E-97
Pfam IPR004007 EMBL-EBI Dak phosphatase domain 1.0E-59
Sequence
Target Sequence 
>C. ELEGANS:183045_S_AT
atctgaatcaactcgacgcgtacgccggtgacggagactgtgggagcacgtttgccggag
cagctgaagcgattcgcaagcagacatcaggcctcaactacactcatccggagacacttc
tcaagcagctttccatcatttttgagcaaaccgtcggaggaaccagtggagcgctgtacg
cgttgatgttcagctcggcggcgcagagtttcgctcaaagatcgcaaagaggagaaaaga
tcgatcgaacgtcgattctggaggcgttggacaaggcgaatcgggcggttcaaaagtacg
gaggcgcgagagtcggagatcggacgatggttgatggattggatgcgatggtcgaggagt
tgagaaagggattcagtgggaatgaggaatcgttggatgcgatcttcgaggcggcggtta
aggcttccgaaaaagcggcgaaagcgactgcaagccaaacggcaaccgtgggccgtgcca
gctacacatcgtcagaagcccaaacgaagcctgatgcaggggccacggcaatctcactct
ggcttagagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTGAATCAACTCGACGCGTACGC 305 33 1169 Antisense
CGCGTACGCCGGTGACGGAGACTGT 369 249 1185 Antisense
GGAGACTGTGGGAGCACGTTTGCCG 40 519 1201 Antisense
TCGCAAGCAGACATCAGGCCTCAAC 302 599 1242 Antisense
TACACTCATCCGGAGACACTTCTCA 478 643 1267 Antisense
GAACCAGTGGAGCGCTGTACGCGTT 489 345 1328 Antisense
GTACGCGTTGATGTTCAGCTCGGCG 262 459 1344 Antisense
TCGAGGCGGCGGTTAAGGCTTCCGA 533 597 1574 Antisense
GCCAGCTACACATCGTCAGAAGCCC 132 279 1645 Antisense
TGATGCAGGGGCCACGGCAATCTCA 190 567 1680 Antisense
GGCAATCTCACTCTGGCTTAGAGCT 169 535 1695 Antisense
Sequence Source Affymetrix Proprietary Database