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[183035_at]

Description ZK180.1 /REP_DB=WormBase Gene ID /WP=CE07619 /TR=Q23442 /GB=AAB52965.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 70      6grp_total > 14      4grp_total > 55      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 365.8
(3)
P 		221.6
(-3)
P 		275.4
(-0.8)
P 		262.4
(-1.3)
P 		144
B 333.5
(3)
P 		268.4
(0.5)
P 		180.7
(-2.8)
P 		174.9
(-3)
P 		159
C 358.4
(3)
P 		250.1
(-0.9)
P 		223.8
(-1.8)
P 		189.7
(-3)
P 		169
avr 352.6
(3) 		246.7
(-1.4) 		226.6
(-2.3) 		209
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 364
1 2 3 4 5 6 range
A 500.2
(3)
P 		464.2
(2.3)
P 		265.1
(-1.4)
P 		290.6
(-0.9)
P 		232.9
(-2)
P 		176.7
(-3)
P 		324
B 393.4
(2.7)
P 		406.6
(3)
P 		263.9
(-0.2)
P 		260.6
(-0.2)
P 		241
(-0.7)
P 		136.6
(-3)
P 		270
C 410.7
(3)
P 		346.9
(1.6)
P 		370
(2.1)
P 		215.9
(-1.4)
P 		268
(-0.2)
P 		145.4
(-3)
P 		265
avr 434.8
(3) 		405.9
(2.4) 		299.7
(0.1) 		255.7
(-0.8) 		247.3
(-1) 		152.9
(-3) 		282
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 133.2
(3)
P 		48.1
(-2.4)
P 		72
(-0.9)
P 		38.4
(-3)
P 		95
B 106.3
(3)
P 		66.3
(-0.5)
P 		41.9
(-2.6)
P 		37.3
(-3)
P 		69
C 118.6
(3)
P 		71.9
(-0.2)
P 		78
(0.2)
P 		32
(-3)
P 		87
avr 119.4
(3) 		62.1
(-1.1) 		64
(-1) 		35.9
(-3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 92.2
(-0.6)
P 		165.1
(3)
P 		57.7
(-2.3)
P 		73.7
(-1.5)
P 		70.3
(-1.7)
P 		43
(-3)
P 		122
B 136.9
(3)
P 		135.4
(2.9)
P 		64.1
(-2.1)
P 		69
(-1.7)
P 		97.9
(0.3)
P 		50.9
(-3)
P 		86
C 152.2
(3)
P 		105.9
(0.4)
P 		94.2
(-0.2)
P 		43.4
(-3)
P 		86.7
(-0.6)
P 		48.7
(-2.7)
P 		109
avr 127.1
(2.4) 		135.5
(3) 		72
(-1.3) 		62
(-2) 		85
(-0.4) 		47.5
(-3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183035_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10835
Sequence Type Exemplar sequence
Representative Public ID ZK180.1 NCBI
Target Description ZK180.1 /REP_DB=WormBase Gene ID /WP=CE07619 /TR=Q23442 /GB=AAB52965.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068178(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068178 NCBI Caenorhabditis elegans ZK180.1 (ZK180.1) mRNA, complete cds. 10/11 None
SNAP00000005942 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4494255:4501055:-1 10/11 None
GENEFINDER00000005956 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4494638:4506602:-1 10/11 None
ZK180.1 ENSEMBL cdna:known chromosome:CEL140:IV:4495928:4499706:-1 gene:ZK180.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4495933-4499712(-) 90.74 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK180.1
Entrez Gene 191240 Entrez gene
SwissProt Q23442 EMBL-EBI
WormBase CE36905 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4965 GABA-B receptor activity inferred from electronic annotation QuickGO AmiGO
8067 metabotropic glutamate, GABA-B-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52965 Hypothetical protein ZK180.1 [Caenorhabditis elegans] ref|NP_500579.2| ZK180.1 [Caenorhabditis elegans] 0.0
blast CAE58539 Hypothetical protein CBG01698 [Caenorhabditis briggsae] 1.0E-173
blast AAB52965 Hypothetical protein ZK180.1 [Caenorhabditis elegans] ref|NP_500579.2| ZK180.1 [Caenorhabditis elegans] 1.0E-172
blast AAB52965 Hypothetical protein ZK180.1 [Caenorhabditis elegans] ref|NP_500579.2| ZK180.1 [Caenorhabditis elegans] 1.0E-167
blast CAE58539 Hypothetical protein CBG01698 [Caenorhabditis briggsae] 1.0E-159
blast CAE58539 Hypothetical protein CBG01698 [Caenorhabditis briggsae] 1.0E-156
blast AAB52966 Hypothetical protein ZK180.2 [Caenorhabditis elegans] ref|NP_500580.2| ZK180.2 [Caenorhabditis elegans] 1.0E-124
blast AAN13882 CG6706-PA, isoform A [Drosophila melanogaster] ref|NP_732658.1| CG6706-PA, isoform A [Drosophila melanogaster] 1.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR000337 EMBL-EBI G-protein coupled receptor family 3, metabotropic glutamate receptor-like 2.0E-36
Pfam IPR000337 EMBL-EBI G-protein coupled receptor family 3, metabotropic glutamate receptor-like 5.0E-38
Pfam IPR000337 EMBL-EBI G-protein coupled receptor family 3, metabotropic glutamate receptor-like 1.0E-47
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 4.5E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500579.2 4 105-127,142-164,185-207,239-261
ZK180.1 4 105-127,142-164,185-207,239-261
SNAP00000005942 6 199-221,236-258,279-301,335-357,370-392,402-424
GENEFINDER00000005956 7 412-434,447-469,484-506,527-549,583-605,618-640,650-672
Sequence
Target Sequence 
>C. ELEGANS:183035_AT
gctggtcacatgggcatttgtctcaccgttttcgtatacggtcactgaacttcctcacat
tccagaagacaatattgtaataatcccagaggtggaaaagtgcaattcttcacattccgg
agtttttcaagcggttctgtatgcagtcaaaggagtcctaatgatcctgggttgcttcct
tgcctgggagactcgacacgtgaatgttccagcactgaacgatagtaaatacattggaac
aagaacgggtcaacgagatgttcagtctcgcttcgttttttgtcattttctcgacgacac
taacgttgtgtctcgtttttgtgccaaagactcaaaattttcgaaaacaccgaatttcat
aatgaaacttttttttaacttttcaaaaaacagttatggcggattcaaaaatttccaaaa
attatcttttggaggatttaatatagtatttgcgagatcccaagtcaaaaaaaaagtaat
cgaattggctcgaaatccggttggcaacgagccaagagcctatcgacggggtcttatgaa
gagtgttgtcgcgaagacttcacagccaatgtcaccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGTCACATGGGCATTTGTCTCA 398 295 609 Antisense
CATTTGTCTCACCGTTTTCGTATAC 310 215 623 Antisense
TCGTATACGGTCACTGAACTTCCTC 560 601 640 Antisense
CTAATGATCCTGGGTTGCTTCCTTG 322 227 766 Antisense
GACTCGACACGTGAATGTTCCAGCA 2 375 798 Antisense
CGAGATGTTCAGTCTCGCTTCGTTT 536 249 862 Antisense
GTCATTTTCTCGACGACACTAACGT 646 465 890 Antisense
TTGTGTCTCGTTTTTGTGCCAAAGA 410 709 914 Antisense
ATTGGCTCGAAATCCGGTTGGCAAC 693 1 1092 Antisense
GGCAACGAGCCAAGAGCCTATCGAC 701 533 1111 Antisense
GAAGACTTCACAGCCAATGTCACCC 497 339 1161 Antisense
Sequence Source Affymetrix Proprietary Database