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[183033_at]

Description F25E5.6 /REP_DB=WormBase Gene ID /WP=CE07140 /TR=O76650 /GB=AAG24077.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 54      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 98.3
(3)
A 		27.5
(-1.8)
A 		51.3
(-0.2)
A 		10.4
(-3)
A 		88
B 85.3
(3)
A 		75
(1.6)
A 		66.3
(0.4)
A 		41.6
(-3)
A 		44
C 72
(2.1)
A 		67.4
(0.8)
A 		54.6
(-3)
A 		75
(3)
A 		20
avr 85.2
(3) 		56.6
(-1) 		57.4
(-0.9) 		42.3
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 105.9
(3)
A 		101.8
(2.5)
A 		63.3
(-2.4)
A 		62
(-2.5)
A 		58.3
(-3)
A 		82.5
(0.1)
A 		48
B 85.6
(2.5)
A 		89.1
(3)
A 		69.6
(0.2)
A 		68.2
(0)
A 		47.1
(-3)
A 		66.3
(-0.3)
A 		42
C 85.7
(2)
A 		91.3
(3)
A 		56.5
(-3)
A 		74.6
(0.1)
A 		62.7
(-1.9)
A 		68.8
(-0.9)
A 		35
avr 92.4
(2.7) 		94.1
(3) 		63.1
(-1.9) 		68.3
(-1.1) 		56
(-3) 		72.5
(-0.4) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 35.8
(3)
A 		6
(-2.2)
A 		13.4
(-0.9)
A 		1.5
(-3)
A 		34
B 27.2
(3)
A 		18.5
(0.1)
A 		15.4
(-0.9)
A 		8.9
(-3)
A 		18
C 23.8
(3)
A 		19.4
(0.6)
A 		19
(0.4)
A 		12.6
(-3)
A 		11
avr 28.9
(3) 		14.6
(-1) 		15.9
(-0.7) 		7.7
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 19.5
(-1.5)
A 		36.2
(3)
A 		13.8
(-3)
A 		15.7
(-2.5)
A 		17.6
(-2)
A 		20.1
(-1.3)
A 		22
B 29.8
(3)
A 		29.7
(3)
A 		16.9
(-3)
A 		18.1
(-2.4)
A 		19.1
(-2)
A 		24.7
(0.6)
A 		13
C 31.8
(3)
A 		27.8
(1.6)
A 		14.4
(-3)
A 		15
(-2.8)
A 		20.3
(-1)
A 		23
(-0)
A 		17
avr 27
(1.4) 		31.2
(3) 		15
(-3) 		16.3
(-2.5) 		19
(-1.5) 		22.6
(-0.2) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183033_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11483
Sequence Type Exemplar sequence
Representative Public ID F25E5.6 NCBI
Target Description F25E5.6 /REP_DB=WormBase Gene ID /WP=CE07140 /TR=O76650 /GB=AAG24077.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028673(11), NM_001028674(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028673 NCBI Caenorhabditis elegans F25E5.6a (F25E5.6) mRNA, complete cds. 11/11 None
NM_001028674 NCBI Caenorhabditis elegans F25E5.6b (F25E5.6) mRNA, complete cds. 11/11 None
SNAP00000010676 ENSEMBL cdna:SNAP chromosome:CEL140:V:7442712:7449304:1 11/11 None
GENEFINDER00000010689 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7442712:7449304:1 11/11 None
F25E5.6a ENSEMBL cdna:known chromosome:CEL140:V:7447371:7449352:1 gene:F25E5.6 11/11 None
F25E5.6b ENSEMBL cdna:known chromosome:CEL140:V:7447867:7449304:1 gene:F25E5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7448014-7449305(+) 100.0 100.0
Public Domain and Genome References
Ensembl F25E5.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAQ62458 Hypothetical protein F25E5.6b [Caenorhabditis elegans] ref|NP_001023845.1| F25E5.6b [Caenorhabditis elegans] 0.0
blast G89101 protein F25E5.6 [imported] - Caenorhabditis elegans 0.0
blast CAB02857 Hypothetical protein C50B6.8 [Caenorhabditis elegans] ref|NP_872233.1| C50B6.8 [Caenorhabditis elegans] 0.0
blast AAN84817 Hypothetical protein F25E5.7 [Caenorhabditis elegans] ref|NP_872235.1| F25E5.7 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 9.0E-16
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 9.4E-21
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 3.6E-49
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 3.6E-49
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 9.4E-21
Sequence
Target Sequence 
>C. ELEGANS:183033_AT
gcccgtatttccctgacaagaagacatggttgttatacgatttaatggtttcaatagagt
acgcaaaaactttcatgttcttccatcaacttggcacaagagatagattgattttgatga
gatatgtttcgttggcgcttatgaatctgcacatttcgtacttcacagtcgccaaaaagt
tcgacaccataattcacccggatggcagcttggcgcctatgaataaagggatggtgtacg
ctgaaacagtgatgtcgatggcgccactaattagatgtaatattcaggaaattgaataca
ttctattgaaagctatttgtctctgtaatccagcagttcccgaactttctacccatgctc
aaaaaattcttgccaaggaacgtgagcaatatgcagatgctctatttgaccattgcattc
gaagtcgaaccaatggaccagctcaatttgccgaacttattagcatggttgacattctgg
aacgacagcagagaatgcaaaaggacttacatctgctgcatattgcccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCCGTATTTCCCTGACAAGAAGAC 259 285 494 Antisense
CATGTTCTTCCATCAACTTGGCACA 438 209 567 Antisense
GAGATATGTTTCGTTGGCGCTTATG 454 397 612 Antisense
GGCGCTTATGAATCTGCACATTTCG 159 543 627 Antisense
CATTTCGTACTTCACAGTCGCCAAA 283 213 645 Antisense
AAGTTCGACACCATAATTCACCCGG 367 153 670 Antisense
GATGGCAGCTTGGCGCCTATGAATA 309 407 694 Antisense
GTGATGTCGATGGCGCCACTAATTA 671 481 742 Antisense
TGTCTCTGTAATCCAGCAGTTCCCG 421 557 811 Antisense
GCTCTATTTGACCATTGCATTCGAA 251 299 892 Antisense
CTTACATCTGCTGCATATTGCCCCT 688 221 999 Antisense
Sequence Source Affymetrix Proprietary Database