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[183017_s_at]

Description F25E5.1 /REP_DB=WormBase Gene ID /WP=CE07135 /TR=O76657 /GB=AAG24072.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 70      6grp_total > 15      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 322.7
(3)
P 		201.5
(-3)
P 		257.2
(-0.2)
P 		307.5
(2.2)
P 		121
B 333.9
(2.9)
P 		335
(3)
P 		277.6
(-3)
P 		282.3
(-2.5)
P 		57
C 378.7
(3)
P 		276
(-2.7)
P 		285.8
(-2.2)
P 		270.8
(-3)
P 		108
avr 345.1
(3) 		270.8
(-3) 		273.5
(-2.8) 		286.9
(-1.7) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 229
1 2 3 4 5 6 range
A 399.5
(3)
P 		389.8
(2.7)
P 		222.5
(-1.6)
P 		241.5
(-1.1)
P 		205.7
(-2.1)
P 		170.6
(-3)
P 		229
B 337.9
(3)
P 		303.6
(1.7)
P 		184.7
(-3)
P 		221.4
(-1.6)
P 		223.9
(-1.5)
P 		224.3
(-1.4)
P 		153
C 373.5
(3)
P 		350.4
(2.2)
P 		323.5
(1.2)
P 		212.1
(-2.9)
P 		242.8
(-1.8)
P 		208.9
(-3)
P 		165
avr 370.3
(3) 		347.9
(2.2) 		243.6
(-1.5) 		225
(-2.2) 		224.1
(-2.2) 		201.3
(-3) 		169
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 117.5
(3)
P 		43.7
(-3)
P 		67.2
(-1.1)
P 		45
(-2.9)
P 		74
B 106.5
(3)
P 		82.7
(-0.1)
P 		64.3
(-2.5)
P 		60.2
(-3)
P 		46
C 125.4
(3)
P 		79.4
(-0.5)
P 		99.6
(1.1)
P 		45.7
(-3)
P 		80
avr 116.5
(3) 		68.6
(-1.3) 		77
(-0.6) 		50.3
(-3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 73.7
(-1)
P 		138.6
(3)
P 		48.5
(-2.6)
P 		61.2
(-1.8)
P 		62.1
(-1.7)
P 		41.5
(-3)
P 		97
B 117.6
(3)
P 		101.1
(1.6)
P 		44.9
(-3)
P 		58.6
(-1.9)
P 		91
(0.8)
P 		83.6
(0.2)
P 		73
C 138.5
(3)
P 		106.9
(1)
P 		82.3
(-0.5)
P 		42.6
(-3)
P 		78.6
(-0.7)
P 		69.9
(-1.3)
P 		96
avr 109.9
(2.5) 		115.5
(3) 		58.6
(-2.6) 		54.1
(-3) 		77.2
(-0.7) 		65
(-1.9) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 183017_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11443
Sequence Type Exemplar sequence
Representative Public ID F25E5.1 NCBI
Target Description F25E5.1 /REP_DB=WormBase Gene ID /WP=CE07135 /TR=O76657 /GB=AAG24072.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072519(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072519 NCBI Caenorhabditis elegans F25E5.1 (F25E5.1) mRNA, complete cds. 11/11 None
SNAP00000010685 ENSEMBL cdna:SNAP chromosome:CEL140:V:7468489:7473450:1 11/11 None
GENEFINDER00000010687 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7468489:7473450:1 11/11 None
F25E5.1 ENSEMBL cdna:known chromosome:CEL140:V:7468489:7473785:1 gene:F25E5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7468489-7473451(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E5.1
Entrez Gene 179135 Entrez gene
SwissProt O76657 EMBL-EBI
WormBase CE07135 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG24072 Hypothetical protein F25E5.1 [Caenorhabditis elegans] ref|NP_504920.1| F25E5.1 [Caenorhabditis elegans] 0.0
blast CAE74156 Hypothetical protein CBG21827 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:183017_S_AT
aaaaatgcccgaagctctcatggaccttcttaagcaatgcatcatagaatcaacttcgaa
gaattattatcacattcgttacctcttcagtcaatatccaatcatgactcaacggtttat
tgctcaataccgtccgggcattattctctggcatagaagtccaattccaaatccccaaat
tcatcatgtcagaattatcaccgaaatgtggccgaagaacgattttgagcgtacaacccg
cattccacggaagaagtttatcaaaaatggaattgttgaagacagcaagttctatatgat
tggatcctatcttcgacgtgatgcgtacaaagacattcactatccggcgcagaaaaccac
aaaactgaccgaattgttcaatatcgatttccccgtaaatggtccaaagaaatctaaaaa
ccagatgatgctaatcacattcccttctcgtgagatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATGCCCGAAGCTCTCATGGAC 361 129 2607 Antisense
TATTATCACATTCGTTACCTCTTCA 504 663 2671 Antisense
TTACCTCTTCAGTCAATATCCAATC 430 681 2685 Antisense
TATTGCTCAATACCGTCCGGGCATT 489 665 2724 Antisense
TCCGGGCATTATTCTCTGGCATAGA 79 595 2739 Antisense
TAGAAGTCCAATTCCAAATCCCCAA 329 639 2760 Antisense
AAATCCCCAAATTCATCATGTCAGA 252 119 2775 Antisense
TTTTGAGCGTACAACCCGCATTCCA 10 675 2829 Antisense
GATCCTATCTTCGACGTGATGCGTA 261 415 2909 Antisense
TACAAAGACATTCACTATCCGGCGC 540 639 2932 Antisense
AATCACATTCCCTTCTCGTGAGATT 317 171 3039 Antisense
Sequence Source Affymetrix Proprietary Database