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[183005_at]

Description F32B5.2 /REP_DB=WormBase Gene ID /WP=CE09850 /TR=O01853 /GB=AAB54207.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 16      4grp_high_8_8 > 41      6grp_total > 14      4grp_total > 54      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 275.2
(3)
P 		111.9
(-3)
P 		121.6
(-2.6)
P 		128.1
(-2.4)
P 		163
B 313.8
(3)
P 		159.5
(-0.2)
P 		107.9
(-1.2)
P 		22.2
(-3)
P 		292
C 289.9
(3)
P 		140.3
(-0.8)
P 		113.1
(-1.5)
P 		51.7
(-3)
A 		238
avr 293
(3) 		137.2
(-1.1) 		114.2
(-1.8) 		67.3
(-3) 		226

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 227
1 2 3 4 5 6 range
A 267.3
(3)
P 		152.6
(-0)
P 		188.9
(0.9)
P 		76.2
(-2.1)
P 		69.5
(-2.2)
P 		40.4
(-3)
M 		227
B 242.6
(3)
P 		218.9
(1.9)
P 		173.5
(-0.1)
P 		187.9
(0.5)
P 		131.5
(-2)
P 		109.9
(-3)
P 		133
C 201.9
(2.5)
P 		215.4
(3)
P 		66.5
(-3)
P 		129.2
(-0.5)
P 		93.1
(-1.9)
P 		81.7
(-2.4)
P 		149
avr 237.3
(3) 		195.6
(1.4) 		143
(-0.5) 		131.1
(-1) 		98
(-2.2) 		77.3
(-3) 		160
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 100.2
(3)
P 		24.3
(-2.6)
P 		31.8
(-2)
P 		18.8
(-3)
P 		81
B 100.1
(3)
P 		39.4
(-0.8)
P 		25
(-1.7)
P 		4.7
(-3)
P 		95
C 96
(3)
P 		40.3
(-0.8)
P 		39.4
(-0.9)
P 		8.7
(-3)
A 		87
avr 98.8
(3) 		34.7
(-1.4) 		32.1
(-1.5) 		10.7
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 49.3
(2.3)
P 		54.3
(3)
P 		41.1
(1.2)
P 		19.3
(-1.7)
P 		21
(-1.5)
P 		9.8
(-3)
M 		45
B 84.4
(3)
P 		72.9
(1.4)
P 		42.1
(-2.8)
P 		49.8
(-1.8)
P 		53.4
(-1.3)
P 		41
(-3)
P 		43
C 74.8
(3)
P 		65.7
(2.1)
P 		16.9
(-3)
P 		26
(-2.1)
P 		30.1
(-1.6)
P 		27.4
(-1.9)
P 		58
avr 69.5
(3) 		64.3
(2.3) 		33.4
(-2) 		31.7
(-2.2) 		34.8
(-1.8) 		26.1
(-3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183005_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.288
Sequence Type Exemplar sequence
Representative Public ID F32B5.2 NCBI
Target Description F32B5.2 /REP_DB=WormBase Gene ID /WP=CE09850 /TR=O01853 /GB=AAB54207.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058625(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058625 NCBI Caenorhabditis elegans F32B5.2 (F32B5.2) mRNA, complete cds. 11/11 None
GENEFINDER00000012568 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2697441:2699907:1 10/11 None
F32B5.2 ENSEMBL cdna:known chromosome:CEL140:I:2697441:2699956:1 gene:F32B5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2697440-2699956(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F32B5.2
Entrez Gene 185196 Entrez gene
SwissProt O01853 EMBL-EBI
WormBase CE36147 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54207 Hypothetical protein F32B5.2 [Caenorhabditis elegans] ref|NP_491026.2| F32B5.2 [Caenorhabditis elegans] 0.0
blast AAB54207 Hypothetical protein F32B5.2 [Caenorhabditis elegans] ref|NP_491026.2| F32B5.2 [Caenorhabditis elegans] 1.0E-177
blast CAE60493 Hypothetical protein CBG04110 [Caenorhabditis briggsae] 1.0E-165
blast CAE60493 Hypothetical protein CBG04110 [Caenorhabditis briggsae] 1.0E-162
Sequence
Target Sequence 
>C. ELEGANS:183005_AT
agaatgtccgattgttaatgtgccaacggtgtatccggaatacgtggcatgcaaatttac
atcaaatgaatggtaccaaattgaatggaaatccacaattcgatacgagacgttcagaaa
tggagagtatggcacgtattgggcaaatgatactcagaactttatagtcaagatgagcga
agaaatgaggagaaatggatcagaacatgtttggctgaaacgattactgaaaattgaatc
gagactgaatggatgtgactccaccgagcttctgatccgatttgcagatgattggacata
ttcatcgaatagtgtcagcttaagttatagaatcttcgaaggatccacaaaaatccattc
cgatcatattggaacattcggagttgaagggtggaaggagatgaatgttccgttgaaaag
aataccaggggatcatgagatttgtgcacagctcctgaacccagctgtatcccgtcacta
cgtgtgctccaaagtagtcgatgctctcgattgtccatttgttctggtttccgtctcctc
cgataaatcatcttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAATGTCCGATTGTTAATGTGCCA 534 75 561 Antisense
GTTAATGTGCCAACGGTGTATCCGG 451 445 574 Antisense
GTGTATCCGGAATACGTGGCATGCA 199 485 589 Antisense
AATCCACAATTCGATACGAGACGTT 196 167 650 Antisense
GGAGAGTATGGCACGTATTGGGCAA 329 517 682 Antisense
GAATGGATGTGACTCCACCGAGCTT 49 333 807 Antisense
GAAGGATCCACAAAAATCCATTCCG 61 335 898 Antisense
GTGTGCTCCAAAGTAGTCGATGCTC 519 487 1042 Antisense
AGTCGATGCTCTCGATTGTCCATTT 552 61 1056 Antisense
TTGTCCATTTGTTCTGGTTTCCGTC 598 707 1071 Antisense
GTCTCCTCCGATAAATCATCTTTTC 491 471 1093 Antisense
Sequence Source Affymetrix Proprietary Database