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[183003_at]

Description B0025.2 /REP_DB=WormBase Gene ID /WP=CE27562 /TR=O01422 /GB=AAB52366.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 08      6grp_cgc > 91      6grp_cgc_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 15.9
(-1.5)
A 		4.3
(-3)
A 		14.9
(-1.7)
A 		51.8
(3)
A 		48
B 22.5
(2.9)
A 		20.4
(2.1)
A 		22.8
(3)
A 		6.6
(-3)
A 		16
C 3.9
(-3)
A 		7.7
(-2.6)
A 		24
(-1.1)
A 		67.3
(3)
P 		63
avr 14.1
(-2.4) 		10.8
(-3) 		20.6
(-1.1) 		41.9
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 9.4
(-2.3)
A 		8.7
(-2.4)
A 		40.3
(3)
A 		22.5
(-0)
A 		5.1
(-3)
A 		13.8
(-1.5)
A 		35
B 4.4
(-3)
A 		29.6
(0.8)
A 		44.2
(3)
A 		7.5
(-2.5)
A 		33.3
(1.4)
A 		41.2
(2.5)
A 		40
C 45.3
(3)
A 		26.3
(-1)
A 		37.3
(1.3)
A 		29.8
(-0.2)
A 		16.5
(-3)
A 		24.6
(-1.3)
A 		29
avr 19.7
(-2.6) 		21.5
(-2.1) 		40.6
(3) 		19.9
(-2.6) 		18.3
(-3) 		26.5
(-0.8) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5.8
(1.4)
A 		0.9
(-3)
A 		3.9
(-0.3)
A 		7.6
(3)
A 		7
B 7.2
(3)
A 		5
(0.7)
A 		5.3
(1)
A 		1.4
(-3)
A 		6
C 1.3
(-3)
A 		2.2
(-2.5)
A 		8.4
(1.2)
A 		11.4
(3)
P 		10
avr 4.8
(0.1) 		2.7
(-3) 		5.9
(1.7) 		6.8
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 1.7
(-2.8)
A 		3.1
(-1.7)
A 		8.8
(3)
A 		5.7
(0.5)
A 		1.5
(-3)
A 		3.4
(-1.4)
A 		7
B 1.5
(-3)
A 		9.9
(0.6)
A 		10.7
(1)
A 		2
(-2.8)
A 		13.5
(2.2)
A 		15.4
(3)
A 		14
C 16.8
(3)
A 		8
(-1.6)
A 		9.5
(-0.8)
A 		6
(-2.6)
A 		5.3
(-3)
A 		8.2
(-1.5)
A 		12
avr 6.7
(-0.5) 		7
(-0.2) 		9.7
(3) 		4.6
(-3) 		6.8
(-0.4) 		9
(2.2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 183003_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1425
Sequence Type Exemplar sequence
Representative Public ID B0025.2 NCBI
Target Description B0025.2 /REP_DB=WormBase Gene ID /WP=CE27562 /TR=O01422 /GB=AAB52366.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170871
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
B0025.4 ENSEMBL cdna:known chromosome:CEL140:I:6035329:6036877:1 gene:B0025.4 None
SNAP00000033236 ENSEMBL cdna:SNAP chromosome:CEL140:I:6035335:6041499:1 None
GENEFINDER00000033241 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6035335:6036416:1 None
NM_170871 NCBI Caenorhabditis elegans B0025.4 (B0025.4) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6033365-6035847(+) 97.78 100.0
Public Domain and Genome References
Gene Title
Ensembl B0025.4
Entrez Gene 172279 Entrez gene
SwissProt Q8WQC3 EMBL-EBI
WormBase CE30120 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV34807 Related to yeast vacuolar protein sorting factor protein 34, isoform c [Caenorhabditis elegans] ref|NP_001020954.1| related to yeast Vacuolar Protein Sorting factor family member (vps-34) [Caenorhabditis elegans] 0.0
blast AAF23184 Related to yeast vacuolar protein sorting factor protein 34, isoform a [Caenorhabditis elegans] ref|NP_491741.1| related to yeast Vacuolar Protein Sorting factor family member (vps-34) [Caenorhabditis elegans] 0.0
blast CAA73142 VPS34 homologue [Caenorhabditis elegans] 0.0
blast T25441 hypothetical protein B0025.2 - Caenorhabditis elegans 3.0E-60
blast AAL50320 Hypothetical protein B0025.4 [Caenorhabditis elegans] ref|NP_740860.1| B0025.4 [Caenorhabditis elegans] 1.0E-16
blast AAW27367 SJCHGC01141 protein [Schistosoma japonicum] 4.0E-14
blast EAA04292 ENSANGP00000013269 [Anopheles gambiae str. PEST] ref|XP_308936.2| ENSANGP00000013269 [Anopheles gambiae str. PEST] 1.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 2.4E-67
Pfam IPR002420 EMBL-EBI Phosphoinositide 3-kinase, C2 domain 9.1E-52
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 3.7E-61
Sequence
Target Sequence 
>C. ELEGANS:183003_AT
gattttctcacaattgtttttgttggaaattttatttttacagttctcaggctggaaatt
gtatctttattcaatgctttttttcacagaaaatgtcaaaagaaaaggatacaagtgaat
taaaaagtgaactcgaaggatggattcggaagaagaatgatattgttgaatcattagaag
cactggaaatgcaaatttataatttcgagggttcatatcttgaagatacagcagaatatg
ggaatgttataaaaggatggggaaactttgcaaatgcaccaccaccatcaaaatctggaa
gaattgataaaaagtttagtaagagaagtgttcgtgatgaagagagacttttttcaaaaa
gttctactacatcaccatatggcacaaagagcacaacaaatggaactggaagtggagaat
ctacaagtggaggggataatggatatacaaaagatgaatctgataataatgatgaagatg
atacggtaattaattttttaaaaaataattttaaaatgtcaaaagttataagaactgtct
tcaacttttccaatttcgtcttaaaatgtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTTCTCACAATTGTTTTTGTTG 446 429 806 Antisense
ATTTTATTTTTACAGTTCTCAGGCT 301 3 834 Antisense
GTTCTCAGGCTGGAAATTGTATCTT 265 443 848 Antisense
GTATCTTTATTCAATGCTTTTTTTC 187 455 866 Antisense
ATAATTTCGAGGGTTCATATCTTGA 178 23 1004 Antisense
GGATGGGGAAACTTTGCAAATGCAC 556 513 1060 Antisense
AACTTTGCAAATGCACCACCACCAT 679 139 1069 Antisense
GTTCTACTACATCACCATATGGCAC 467 443 1166 Antisense
GTGGAGAATCTACAAGTGGAGGGGA 66 489 1217 Antisense
TATAAGAACTGTCTTCAACTTTTCC 346 639 1332 Antisense
TTTCCAATTTCGTCTTAAAATGTCA 10 669 1352 Antisense
Sequence Source Affymetrix Proprietary Database