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[182983_s_at]

Description K02E7.7 /REP_DB=WormBase Gene ID /WP=CE11634 /TR=O17251 /GB=AAB71028.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 30      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 59.7
(0.3)
A 		39.9
(-1)
A 		11.1
(-3)
A 		98.5
(3)
A 		87
B 117.4
(3)
P 		46.8
(-1.1)
A 		36.3
(-1.7)
A 		13.1
(-3)
A 		104
C 77.6
(1.1)
A 		49.9
(-1.1)
A 		27.4
(-3)
A 		100.1
(3)
A 		73
avr 84.9
(3) 		45.5
(-0.9) 		24.9
(-3) 		70.6
(1.6) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 55.9
(0.7)
A 		59.9
(1)
A 		74.2
(2.1)
A 		6.9
(-3)
A 		27.3
(-1.5)
A 		86.1
(3)
A 		79
B 51
(0.9)
A 		55.8
(1.3)
A 		59.3
(1.6)
A 		3.8
(-3)
A 		26.4
(-1.1)
A 		76.9
(3)
A 		73
C 67.3
(1.8)
A 		82.7
(3)
A 		64.4
(1.5)
A 		31.4
(-1.1)
A 		7.3
(-3)
A 		79.8
(2.8)
A 		75
avr 58.1
(1) 		66.1
(1.7) 		66
(1.7) 		14
(-3) 		20.3
(-2.4) 		80.9
(3) 		67
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 21.7
(3)
A 		8.7
(-1.1)
A 		2.9
(-3)
A 		14.4
(0.7)
A 		19
B 37.4
(3)
P 		11.6
(-1.5)
A 		8.4
(-2)
A 		2.8
(-3)
A 		35
C 25.7
(3)
A 		14.4
(-1.2)
A 		9.5
(-3)
A 		16.9
(-0.3)
A 		16
avr 28.3
(3) 		11.6
(-1.7) 		6.9
(-3) 		11.4
(-1.7) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 10.3
(-0.4)
A 		21.3
(3)
A 		16.2
(1.4)
A 		1.7
(-3)
A 		8.2
(-1)
A 		21
(2.9)
A 		20
B 17.7
(0.6)
A 		18.6
(0.8)
A 		14.4
(-0.1)
A 		1
(-3)
A 		10.7
(-0.9)
A 		28.7
(3)
A 		28
C 25
(2.6)
A 		25.2
(2.6)
A 		16.4
(0.5)
A 		6.3
(-2)
A 		2.4
(-3)
A 		26.7
(3)
A 		24
avr 17.7
(0.9) 		21.7
(2) 		15.7
(0.4) 		3
(-3) 		7.1
(-1.9) 		25.5
(3) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182983_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5422
Sequence Type Exemplar sequence
Representative Public ID K02E7.7 NCBI
Target Description K02E7.7 /REP_DB=WormBase Gene ID /WP=CE11634 /TR=O17251 /GB=AAB71028.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061499(11), NM_061474(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061499 NCBI Caenorhabditis elegans K02E7.7 (K02E7.7) mRNA, complete cds. 11/11 None
NM_061474 NCBI Caenorhabditis elegans C32B5.1a (C32B5.1) mRNA, complete cds. 11/11 A 
C32B5.1a ENSEMBL cdna:known chromosome:CEL140:II:986024:988616:1 gene:C32B5.1 11/11 A 
K02E7.7 ENSEMBL cdna:known chromosome:CEL140:II:1055030:1056020:1 gene:K02E7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173477 2 1 NM_061474 176668_at (11), 182983_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1055028-1056019(+) 100.0 100.0
chrII:986022-987013(+) 99.32 99.32
Public Domain and Genome References
Gene Title
Ensembl C32B5.1
K02E7.7
Entrez Gene 186888 Entrez gene
SwissProt O17251 EMBL-EBI
WormBase CE11634 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493875 C32B5.1a [Caenorhabditis elegans] gb|AAK71358.1| Hypothetical protein C32B5.1a [Caenorhabditis elegans] 0.0
blast NP_872007 C32B5.1b [Caenorhabditis elegans] gb|AAN63439.1| Hypothetical protein C32B5.1b [Caenorhabditis elegans] 1.0E-162
blast AAB71028 Hypothetical protein K02E7.7 [Caenorhabditis elegans] ref|NP_493900.1| K02E7.7 [Caenorhabditis elegans] 3.0E-93
blast NP_493875 C32B5.1a [Caenorhabditis elegans] gb|AAK71358.1| Hypothetical protein C32B5.1a [Caenorhabditis elegans] 4.0E-64
blast AAB71028 Hypothetical protein K02E7.7 [Caenorhabditis elegans] ref|NP_493900.1| K02E7.7 [Caenorhabditis elegans] 4.0E-64
Sequence
Target Sequence 
>C. ELEGANS:182983_S_AT
gagactactcaatactttacggaagccttttagagttttccgccgatttttaaactcttt
aactactcgaaaaatagaaaacaaatcattcaccgagcagctaactgaggaagcagatga
atttatattttctagctcaaaatgttggcaaccttccagagatgaactctctcaacgatg
cccatcaccccaagattcctatattagcacttcctcaaatttcagcactgtttccgaaaa
aacttccagttttggcatcccagaggttctcagcaagagtgaacatttcacaaaaagcca
aaagatgatgacgtatccatgcctgaagtctgtgctcgttaacttcgatcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACTACTCAATACTTTACGGAAG 251 393 15 Antisense
AAATCATTCACCGAGCAGCTAACTG 507 117 97 Antisense
AAATGTTGGCAACCTTCCAGAGATG 179 121 154 Antisense
GAGATGAACTCTCTCAACGATGCCC 409 395 173 Antisense
CCATCACCCCAAGATTCCTATATTA 237 271 196 Antisense
GATTCCTATATTAGCACTTCCTCAA 440 433 208 Antisense
ACTTCCTCAAATTTCAGCACTGTTT 60 105 223 Antisense
AAACTTCCAGTTTTGGCATCCCAGA 29 129 254 Antisense
TTGGCATCCCAGAGGTTCTCAGCAA 423 709 266 Antisense
GTATCCATGCCTGAAGTCTGTGCTC 357 455 327 Antisense
GTCTGTGCTCGTTAACTTCGATCCA 565 471 342 Antisense
Sequence Source Affymetrix Proprietary Database