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[182976_at]

Description C24G7.4 /REP_DB=WormBase Gene ID /WP=CE08354 /TR=P91100 /GB=AAB42340.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 76      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_total > 67      4grp_total > 10      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 700
1 2 3 4 range
A 91.8
(-3)
P 		221.7
(-1.9)
P 		136.8
(-2.6)
P 		791.3
(3)
P 		700
B 117.6
(-3)
P 		150.7
(-2.7)
P 		131.5
(-2.9)
P 		732.9
(3)
P 		615
C 109.3
(-3)
P 		170.8
(-2.3)
P 		140.2
(-2.6)
P 		625.9
(3)
P 		517
avr 106.2
(-3) 		181.1
(-2.3) 		136.2
(-2.7) 		716.7
(3) 		611

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 416
1 2 3 4 5 6 range
A 125.6
(-2.1)
P 		78.5
(-2.8)
P 		135.5
(-2)
P 		67.9
(-3)
P 		69.3
(-3)
A 		462
(3)
P 		394
B 95.2
(-2.3)
P 		94.9
(-2.3)
P 		91.2
(-2.3)
P 		52.9
(-3)
P 		85.2
(-2.4)
P 		394.6
(3)
P 		342
C 81.4
(-2.5)
P 		131.1
(-1.7)
P 		124.4
(-1.8)
P 		46
(-3)
A 		54.8
(-2.9)
M 		449.9
(3)
P 		404
avr 100.7
(-2.3) 		101.5
(-2.3) 		117
(-2) 		55.6
(-3) 		69.8
(-2.8) 		435.5
(3) 		380
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 33.4
(-3)
P 		48.1
(-1.9)
P 		35.8
(-2.8)
P 		115.8
(3)
P 		82
B 37.5
(-2.7)
P 		37.2
(-2.7)
P 		30.5
(-3)
P 		156.2
(3)
P 		126
C 36.2
(-3)
P 		49.1
(-1.9)
P 		48.9
(-1.9)
P 		105.5
(3)
P 		69
avr 35.7
(-3) 		44.8
(-2.4) 		38.4
(-2.8) 		125.8
(3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 23.1
(-2.6)
P 		27.9
(-2.3)
P 		29.5
(-2.2)
P 		17.2
(-3)
P 		20.9
(-2.8)
A 		112.5
(3)
P 		95
B 33.1
(-2.1)
P 		31.6
(-2.2)
P 		22.2
(-2.6)
P 		14
(-3)
P 		34.6
(-2.1)
P 		147.1
(3)
P 		133
C 30.2
(-2.1)
P 		40
(-1.7)
P 		31.7
(-2)
P 		9.3
(-3)
A 		17.7
(-2.6)
M 		150.6
(3)
P 		141
avr 28.8
(-2.3) 		33.2
(-2) 		27.8
(-2.3) 		13.5
(-3) 		24.4
(-2.5) 		136.7
(3) 		123
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182976_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.380
Sequence Type Exemplar sequence
Representative Public ID C24G7.4 NCBI
Target Description C24G7.4 /REP_DB=WormBase Gene ID /WP=CE08354 /TR=P91100 /GB=AAB42340.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058892(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058892 NCBI Caenorhabditis elegans C24G7.4 (C24G7.4) mRNA, complete cds. 11/11 None
SNAP00000001459 ENSEMBL cdna:SNAP chromosome:CEL140:I:4094180:4098111:1 11/11 None
GENEFINDER00000001479 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4094180:4103530:1 11/11 None
C24G7.4 ENSEMBL cdna:known chromosome:CEL140:I:4094611:4098348:1 gene:C24G7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4094610-4098111(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C24G7.4
Entrez Gene 171992 Entrez gene
SwissProt P91100 EMBL-EBI
WormBase CE08354 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23916.1.S1_AT similar to amiloride-sensitive cation channel 4, pituitary cfa
HG-U133_PLUS_2:221226_S_AT amiloride-sensitive cation channel 4, pituitary hs
HG-U133A:221226_S_AT amiloride-sensitive cation channel 4, pituitary hs
HG-FOCUS:221226_S_AT amiloride-sensitive cation channel 4, pituitary hs
HG-U133A_2:221226_S_AT amiloride-sensitive cation channel 4, pituitary hs
HG-U95B:42027_AT amiloride-sensitive cation channel 4, pituitary hs
HU35KSUBC:RC_AA235984_AT amiloride-sensitive cation channel 4, pituitary hs
U133_X3P:G8922082_3P_A_AT amiloride-sensitive cation channel 4, pituitary hs
MOE430B:1437091_AT amiloride-sensitive cation channel 4, pituitary mm
MOUSE430_2:1437091_AT amiloride-sensitive cation channel 4, pituitary mm
RAE230A:1370206_AT amiloride-sensitive cation channel 4, pituitary rn
RAT230_2:1370206_AT amiloride-sensitive cation channel 4, pituitary rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42340 Hypothetical protein C24G7.4 [Caenorhabditis elegans] ref|NP_491293.1| C24G7.4 [Caenorhabditis elegans] 0.0
blast CAE66722 Hypothetical protein CBG12068 [Caenorhabditis briggsae] 0.0
blast CAE61113 Hypothetical protein CBG04870 [Caenorhabditis briggsae] 0.0
blast CAE61116 Hypothetical protein CBG04873 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491293.1 2 82-104,489-511
C24G7.4 2 82-104,489-511
SNAP00000001459 2 102-124,509-531
GENEFINDER00000001479 2 102-124,519-541
Sequence
Target Sequence 
>C. ELEGANS:182976_AT
cacatcgtattctcaggttcaagccacctcattaactgaaattctatcagacattggtgg
taatatgggaatgtttttgggaatgtcagtgatcacaatcactgaactttctctattctt
ttcgaaaattttctggattatggtctcgaagcgaagaaggcagtacatgtactccaagaa
aactcatgaaaaagaaaaagagcatcaattggatgaagcggttaaggagtttcaagagag
aagatccagaagaaactcgagagaaaatatctcagcactcgggcactacagtaatcgaat
cacgcctgttgatgattttcagactaagttcggatacaagaatgcgttttctgaagggaa
tatgtcgaattctagtttagatagtgtgatggagctgaaattcgacatcaacgagctgcg
tcgtcaactgaaccagccttcaactgacggaatcgcccgtatccgccttccccaccaaac
gagtcgacagaattctactgaaaactactattcatcctctccaccaatcttcacaattga
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACATCGTATTCTCAGGTTCAAGCC 214 193 1407 Antisense
GGTTCAAGCCACCTCATTAACTGAA 219 505 1422 Antisense
CACTGAACTTTCTCTATTCTTTTCG 153 197 1506 Antisense
AAAATATCTCAGCACTCGGGCACTA 227 133 1670 Antisense
GCACTACAGTAATCGAATCACGCCT 342 315 1689 Antisense
AAATTCGACATCAACGAGCTGCGTC 517 115 1804 Antisense
GCTGCGTCGTCAACTGAACCAGCCT 380 297 1821 Antisense
GAACCAGCCTTCAACTGACGGAATC 639 345 1836 Antisense
TTCCCCACCAAACGAGTCGACAGAA 7 699 1874 Antisense
TGAAAACTACTATTCATCCTCTCCA 513 573 1905 Antisense
CTCTCCACCAATCTTCACAATTGAG 26 231 1923 Antisense
Sequence Source Affymetrix Proprietary Database