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[182975_s_at]

Description C04F5.1 /REP_DB=WormBase Gene ID /WP=CE19665 /TR=Q9GZC8 /GB=AAF98593.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 92      6grp_total > 84      4grp_total > 60      6grp_cgc > 87      6grp_high1_sub > 04_05      6grp_cgc_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 114.8
(0)
P 		142.5
(1.7)
P 		163.9
(3)
P 		65.4
(-3)
A 		99
B 101.4
(-0.5)
P 		142.3
(3)
P 		126.1
(1.6)
P 		72.1
(-3)
A 		70
C 158
(3)
M 		101.2
(-3)
P 		144.8
(1.6)
P 		112.5
(-1.8)
A 		57
avr 124.7
(1) 		128.7
(1.4) 		144.9
(3) 		83.3
(-3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 146.8
(-3)
M 		169.5
(-2)
P 		184.1
(-1.3)
P 		227.2
(0.7)
P 		278.5
(3)
P 		231.6
(0.9)
P 		132
B 177.4
(-3)
P 		225.3
(0.2)
P 		200.9
(-1.5)
P 		268.5
(3)
P 		227.2
(0.3)
P 		184.2
(-2.6)
P 		91
C 159.2
(-3)
P 		163.4
(-2.7)
P 		176.6
(-1.9)
P 		257.2
(3)
P 		234.1
(1.6)
P 		230.6
(1.4)
P 		98
avr 161.1
(-3) 		186.1
(-1.3) 		187.2
(-1.3) 		251
(3) 		246.6
(2.7) 		215.5
(0.6) 		90
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 41.8
(2.8)
P 		30.9
(0.8)
P 		42.9
(3)
P 		9.6
(-3)
A 		33
B 32.3
(2.1)
P 		35.1
(3)
P 		29.2
(1.2)
P 		15.4
(-3)
A 		20
C 52.3
(3)
M 		29.1
(-1.2)
P 		50.5
(2.7)
P 		19
(-3)
A 		33
avr 42.1
(3) 		31.7
(0.7) 		40.9
(2.7) 		14.7
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 27.1
(-3)
M 		60.3
(0.5)
P 		40.1
(-1.6)
P 		57.6
(0.2)
P 		84
(3)
P 		56.4
(0.1)
P 		57
B 61.7
(-1.2)
P 		75
(0.6)
P 		48.8
(-3)
P 		71.1
(0.1)
P 		92.3
(3)
P 		68.7
(-0.3)
P 		44
C 59
(-0.4)
P 		49.9
(-2.1)
P 		44.9
(-3)
P 		51.7
(-1.7)
P 		75.7
(2.7)
P 		77.2
(3)
P 		32
avr 49.3
(-2.3) 		61.7
(-0.4) 		44.6
(-3) 		60.1
(-0.6) 		84
(3) 		67.4
(0.5) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182975_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15760
Sequence Type Exemplar sequence
Representative Public ID C04F5.1 NCBI
Target Description C04F5.1 /REP_DB=WormBase Gene ID /WP=CE19665 /TR=Q9GZC8 /GB=AAF98593.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071971(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071971 NCBI Caenorhabditis elegans Systemic RNA Interference Defective family member (sid-1) (sid-1) mRNA, complete cds. 11/11 None
SNAP00000034406 ENSEMBL cdna:SNAP chromosome:CEL140:V:5107799:5126414:1 11/11 None
GENEFINDER00000034410 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5119790:5126414:1 11/11 None
C04F5.1 ENSEMBL cdna:known chromosome:CEL140:V:5119783:5126726:1 gene:C04F5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5119789-5126414(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sid-1
Ensembl C04F5.1
Entrez Gene 178900 Entrez gene
SwissProt Q9GZC8 EMBL-EBI
WormBase CE30331 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5886 plasma membrane inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF98593 Systemic rna interference defective protein 1 [Caenorhabditis elegans] ref|NP_504372.2| Systemic RNA Interference Defective family member (sid-1) [Caenorhabditis elegans] gb|AAL78657.1| systemic RNAi enabling protein SID-1 [Caenorhabditis elegans] sp|Q9GZC8|SID1_CAEEL Systemic RNA interference defective protein 1 precursor (Systemic RNAi enabling protein) 0.0
blast CAE64078 Hypothetical protein CBG08682 [Caenorhabditis briggsae] 0.0
blast T33773 hypothetical protein C04F5.1 - Caenorhabditis elegans 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504372.2 11 314-336,425-447,483-502,509-531,546-568,575-597,601-620,633-655,660-679,692-711,742-761
C04F5.1 11 314-336,425-447,483-502,509-531,546-568,575-597,601-620,633-655,660-679,692-711,742-761
SNAP00000034406 11 424-446,535-557,593-612,619-641,656-678,685-707,711-730,743-765,770-789,802-821,852-871
GENEFINDER00000034410 11 314-336,425-447,483-502,509-531,546-568,575-597,601-620,633-655,660-679,692-711,742-761
Sequence
Target Sequence 
>C. ELEGANS:182975_S_AT
tctccatcaccagcctatacaaatatcctactagttggagttgtctccttgaactttcta
atatctgcattctccaaaacatcatatgtccgattcatcatcgctgtaattcatgtcatt
gtcgttggatcgatctgtttggcaaaggaaagatccttgggatcggaaaaattaaaaact
cgatttttcatcatggccttctcgatgggaaatttcgcagcaatcgtgatgtatctgacg
ctttcggcgtttcatttgaatcaaatagccacgtattgctttattataaattgtatcatg
tatctgatgtactatggatgcatgaaagttttacatagcgagagaataacgtcgaaggct
aaactttgtggagctctgtcactgctcgcgtgggctgttgccggatttttcttctttcaa
gatgatacagattggacgagatctgcggcggcgagccgagcactcaacaagccatgcctg
ctactcggcttcttcggttcccacgatttatggcacatcttcggagcattggccggtctt
ttcacattcattttcgtctcctttgttgatgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCCATCACCAGCCTATACAAATA 588 609 1342 Antisense
GGAGTTGTCTCCTTGAACTTTCTAA 547 515 1378 Antisense
ATATCTGCATTCTCCAAAACATCAT 351 21 1402 Antisense
ATATGTCCGATTCATCATCGCTGTA 502 21 1425 Antisense
AACTCGATTTTTCATCATGGCCTTC 505 141 1518 Antisense
GTATCTGACGCTTTCGGCGTTTCAT 16 455 1572 Antisense
AACTTTGTGGAGCTCTGTCACTGCT 122 139 1703 Antisense
CTTCTTCGGTTCCCACGATTTATGG 135 221 1830 Antisense
GGCACATCTTCGGAGCATTGGCCGG 30 535 1853 Antisense
GGTCTTTTCACATTCATTTTCGTCT 530 503 1876 Antisense
ATTTTCGTCTCCTTTGTTGATGATG 224 15 1891 Antisense
Sequence Source Affymetrix Proprietary Database