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[182973_at] 7TM chemoreceptor, srb family

Description C27D6.6 /REP_DB=WormBase Gene ID /WP=CE06886 /GEN=srb-5 /GB=AAK68207.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 12      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 22.7
(-2.1)
A 		9.5
(-3)
A 		13.7
(-2.7)
A 		95
(3)
A 		86
B 45.5
(2.5)
A 		5.9
(-3)
A 		8.1
(-2.7)
A 		48.8
(3)
A 		43
C 39.6
(-1.5)
A 		55.4
(-0.3)
A 		19.9
(-3)
A 		98.7
(3)
P 		79
avr 35.9
(-1) 		23.6
(-2.1) 		13.9
(-3) 		80.8
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 86.4
(3)
A 		9.2
(-3)
A 		58.7
(0.8)
A 		37.1
(-0.8)
A 		52.3
(0.3)
A 		57.7
(0.8)
A 		77
B 42.3
(-0.5)
A 		35.2
(-1.5)
A 		68.4
(3)
P 		43.3
(-0.4)
P 		41.3
(-0.6)
P 		23.8
(-3)
A 		45
C 34.7
(-0.3)
A 		59.7
(1.8)
P 		3.7
(-3)
A 		73.8
(3)
A 		19.8
(-1.6)
A 		6.3
(-2.8)
A 		70
avr 54.5
(3) 		34.7
(-1.7) 		43.6
(0.4) 		51.4
(2.3) 		37.8
(-1) 		29.3
(-3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 8.3
(0.2)
A 		2.1
(-3)
A 		3.6
(-2.2)
A 		13.9
(3)
A 		12
B 14.5
(3)
A 		1.4
(-3)
A 		1.9
(-2.8)
A 		10.4
(1.1)
A 		13
C 13.1
(0.8)
A 		15.9
(2.6)
A 		6.9
(-3)
A 		16.6
(3)
P 		10
avr 12
(2) 		6.5
(-1.5) 		4.1
(-3) 		13.6
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 15.9
(3)
A 		3.3
(-3)
A 		12.8
(1.5)
A 		9.4
(-0.1)
A 		15.8
(3)
A 		14
(2.1)
A 		13
B 14.7
(1.4)
A 		11.7
(-0.9)
A 		16.6
(2.8)
P 		11.5
(-1)
P 		16.8
(3)
P 		8.9
(-3)
A 		8
C 12.9
(1.2)
A 		18.2
(3)
P 		0.9
(-3)
A 		14.8
(1.8)
A 		6.4
(-1.1)
A 		2.1
(-2.6)
A 		17
avr 14.5
(3) 		11.1
(-0.3) 		10.1
(-1.3) 		11.9
(0.5) 		13
(1.5) 		8.3
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182973_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4237
Sequence Type Exemplar sequence
Representative Public ID C27D6.6 NCBI
Target Description C27D6.6 /REP_DB=WormBase Gene ID /WP=CE06886 /GEN=srb-5 /GB=AAK68207.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062560(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062560 NCBI Caenorhabditis elegans Serpentine Receptor, class B (beta) family member (srb-5) (srb-5) mRNA, complete cds. 11/11 None
GENEFINDER00000035750 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5159764:5163457:-1 9/11 None
C27D6.6 ENSEMBL cdna:known chromosome:CEL140:II:5161976:5163364:-1 gene:C27D6.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5161973-5163362(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srb family
Gene Symbol srb-5
Ensembl C27D6.6
Entrez Gene 191787 Entrez gene
SwissProt Q95ZY4 EMBL-EBI
WormBase CE06886 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast T15653 hypothetical protein C27D6.2 - Caenorhabditis elegans 0.0
blast NP_494961 Serpentine Receptor, class B (beta) family member (srb-5) [Caenorhabditis elegans] gb|AAK68207.1| Serpentine receptor, class b (beta) protein 5 [Caenorhabditis elegans] sp|Q95ZY4|SRB5_CAEEL Serpentine receptor class beta-5 (Protein srb-5) prf||2123261T chemosensory receptor 1.0E-175
blast NP_001021995 C27D6.11 [Caenorhabditis elegans] gb|AAY55860.1| Hypothetical protein C27D6.11 [Caenorhabditis elegans] 1.0E-154
blast CAA16343 Hypothetical protein Y38H8A.4 [Caenorhabditis elegans] ref|NP_502595.1| Y38H8A.4 [Caenorhabditis elegans] 1.0E-143
blast CAE59056 Hypothetical protein CBG02339 [Caenorhabditis briggsae] 1.0E-132
blast T15653 hypothetical protein C27D6.2 - Caenorhabditis elegans 1.0E-128
Protein Families
Method ID Description E-Value
hanks 2.2.11 CaMK Group; CaMK II KIN1/SNF1/Nim1; YKL453 1.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 1.0E-126
Pfam IPR002184 EMBL-EBI Integral membrane protein Srb, nematode type 1.0E-126
Pfam IPR000719 EMBL-EBI Protein kinase 4.7E-72
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494961.1 7 23-45,66-88,108-130,142-164,189-211,240-262,277-299
C27D6.6 7 23-45,66-88,108-130,142-164,189-211,240-262,277-299
GENEFINDER00000035750 7 40-58,73-90,97-115,120-142,155-177,203-225,254-276
Sequence
Target Sequence 
>C. ELEGANS:182973_AT
actattggggcctattctgtgctttattcttgttgctccaaactttgtcgtattttattt
tttgtttcgagatgaacaattcacggattctttcatctcgtttttagttctcccaaatac
accagcggttcaatttaataactatctttggttcttgctttatgcaaaaattggaaattt
ttgttgcaattgtgtgctcttgatatttcacaaaagatttaaaaatacatacttgaaaaa
gaaaacttcattatcagttagatatgcattggaagaaatatcaaattcatcgaaattcac
attaattctcacattcacccatcttgttttctttggagcatacactattggaagtattct
agttcgaactcttggtgaatcttttttcggaaactttctcaatttctatgttgcaagagg
tgtaaattgtgcagttccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTATTGGGGCCTATTCTGTGCTTT 217 103 435 Antisense
TCTGTGCTTTATTCTTGTTGCTCCA 201 609 450 Antisense
GTTGCTCCAAACTTTGTCGTATTTT 623 439 466 Antisense
ACAATTCACGGATTCTTTCATCTCG 165 115 510 Antisense
TTAGTTCTCCCAAATACACCAGCGG 613 677 538 Antisense
ACTATCTTTGGTTCTTGCTTTATGC 491 101 575 Antisense
GTTGCAATTGTGTGCTCTTGATATT 401 439 617 Antisense
TCACATTAATTCTCACATTCACCCA 24 629 731 Antisense
CATTCACCCATCTTGTTTTCTTTGG 675 215 746 Antisense
GAACTCTTGGTGAATCTTTTTTCGG 506 349 800 Antisense
GAGGTGTAAATTGTGCAGTTCCGAC 146 401 851 Antisense
Sequence Source Affymetrix Proprietary Database