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[182953_at]

Description H38K22.4 /REP_DB=WormBase Gene ID /WP=CE18832 /TR=Q9XXA8 /GB=CAA19706.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 35      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 6.3
(-2.8)
A 		50.4
(3)
A 		4.7
(-3)
A 		20.1
(-1)
A 		46
B 6.5
(-3)
A 		43.8
(3)
A 		8.8
(-2.6)
A 		7
(-2.9)
A 		37
C 43.3
(1.5)
A 		13.3
(-2.4)
A 		8.8
(-3)
A 		54.3
(3)
A 		46
avr 18.7
(-0.6) 		35.8
(3) 		7.4
(-3) 		27.1
(1.2) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 10.6
(-1.9)
A 		3.9
(-3)
A 		39.7
(3)
A 		4.6
(-2.9)
A 		7.1
(-2.5)
A 		27.3
(0.9)
A 		36
B 8.4
(-3)
A 		28
(1.7)
A 		29.9
(2.1)
A 		33.2
(2.9)
A 		21.1
(0)
A 		33.6
(3)
A 		25
C 19.7
(-0.1)
A 		33.8
(3)
A 		30.4
(2.3)
A 		9.2
(-2.4)
A 		6.5
(-3)
A 		6.3
(-3)
A 		28
avr 12.9
(-2.6) 		21.9
(-0.2) 		33.3
(3) 		15.7
(-1.9) 		11.6
(-3) 		22.4
(-0) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 2.3
(-2.3)
A 		10.9
(3)
A 		1.2
(-3)
A 		2.9
(-1.9)
A 		10
B 2.1
(-2.6)
A 		10.8
(3)
A 		2
(-2.7)
A 		1.5
(-3)
A 		9
C 14.3
(3)
A 		3.8
(-2.6)
A 		3.1
(-3)
A 		9.2
(0.3)
A 		11
avr 6.2
(0.8) 		8.5
(3) 		2.1
(-3) 		4.5
(-0.8) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.9
(-2.4)
A 		1.4
(-2.8)
A 		8.7
(3)
A 		1.2
(-3)
A 		2.1
(-2.3)
A 		6.6
(1.3)
A 		8
B 2.9
(-3)
A 		9.3
(1)
A 		7.3
(-0.3)
A 		8.8
(0.7)
A 		8.6
(0.6)
A 		12.5
(3)
A 		10
C 7.3
(0.9)
A 		10.3
(3)
A 		7.7
(1.2)
A 		1.8
(-3)
A 		2.1
(-2.8)
A 		2.1
(-2.8)
A 		9
avr 4
(-2.9) 		7
(1.7) 		7.9
(3) 		3.9
(-3) 		4.3
(-2.4) 		7.1
(1.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182953_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6756
Sequence Type Exemplar sequence
Representative Public ID H38K22.4 NCBI
Target Description H38K22.4 /REP_DB=WormBase Gene ID /WP=CE18832 /TR=Q9XXA8 /GB=CAA19706.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065468(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065468 NCBI Caenorhabditis elegans H38K22.4 (H38K22.4) mRNA, complete cds. 11/11 None
H38K22.4 ENSEMBL cdna:known chromosome:CEL140:III:4322786:4323436:-1 gene:H38K22.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4322785-4323436(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H38K22.4
Entrez Gene 186808 Entrez gene
SwissProt Q9XXA8 EMBL-EBI
WormBase CE18832 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA19706 Hypothetical protein H38K22.4 [Caenorhabditis elegans] ref|NP_497869.1| H38K22.4 [Caenorhabditis elegans] 7.0E-46
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497869.1 1 35-57
H38K22.4 1 35-57
Sequence
Target Sequence 
>C. ELEGANS:182953_AT
tcccctactcgacgggaggagccgtttctcaaacagatggaaagtggaagagctccccgg
gtgaaacagtatgtctggatgagagaggcaggcacattttcaggctcattcgcttgcctg
cttgttttatccattttggtgggaattgtaagaggcgtaggctaccttcaaggcaggcat
gaaatgtgggcaatttttaagaagctttcagatgattgctgtgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCCTACTCGACGGGAGGAGCCGT 570 555 19 Antisense
GGAGGAGCCGTTTCTCAAACAGATG 590 515 33 Antisense
TGGAAAGTGGAAGAGCTCCCCGGGT 608 541 56 Antisense
GCTCCCCGGGTGAAACAGTATGTCT 310 299 70 Antisense
GAGGCAGGCACATTTTCAGGCTCAT 481 405 103 Antisense
TTTTCAGGCTCATTCGCTTGCCTGC 415 675 115 Antisense
TTGTTTTATCCATTTTGGTGGGAAT 421 705 140 Antisense
GGTGGGAATTGTAAGAGGCGTAGGC 619 497 156 Antisense
GCGTAGGCTACCTTCAAGGCAGGCA 286 287 173 Antisense
AAGGCAGGCATGAAATGTGGGCAAT 708 159 188 Antisense
GAAGCTTTCAGATGATTGCTGTGCG 186 343 219 Antisense
Sequence Source Affymetrix Proprietary Database