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[182952_at]

Description F35G12.5 /REP_DB=WormBase Gene ID /WP=CE00974 /TR=Q20055 /GB=CAA86331.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 96      4grp_high_8_8 > 21      6grp_highest_nonorm > 90      6grp_total > 86      4grp_total > 10      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 95.5
(-2.4)
P 		79.6
(-3)
M 		108.4
(-1.9)
P 		243.3
(3)
P 		164
B 115.3
(-3)
P 		127.4
(-2)
P 		121.4
(-2.5)
P 		189.7
(3)
P 		74
C 92.9
(-3)
P 		137.8
(-1)
P 		100.2
(-2.7)
P 		230.4
(3)
P 		138
avr 101.2
(-3) 		114.9
(-2.3) 		110
(-2.6) 		221.1
(3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 154.7
(0.6)
P 		96.6
(-3)
P 		110.3
(-2.2)
P 		145.3
(-0)
P 		173.9
(1.7)
P 		194.5
(3)
P 		98
B 110.4
(-2.9)
P 		108.9
(-3)
P 		159.7
(-1)
P 		170.9
(-0.5)
P 		181.7
(-0.1)
P 		260.5
(3)
P 		152
C 84.2
(-3)
P 		138.2
(-0.9)
P 		138.7
(-0.9)
P 		142.3
(-0.7)
P 		169.9
(0.3)
P 		238.6
(3)
P 		154
avr 116.4
(-2.9) 		114.6
(-3) 		136.2
(-1.9) 		152.8
(-1) 		175.2
(0.1) 		231.2
(3) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 34.8
(2.7)
P 		17.3
(-3)
M 		28.3
(0.6)
P 		35.6
(3)
P 		18
B 36.8
(1.2)
P 		31.5
(-1.3)
P 		28.1
(-3)
P 		40.4
(3)
P 		12
C 30.7
(-3)
P 		39.6
(3)
P 		34.9
(-0.2)
P 		38.8
(2.5)
P 		9
avr 34.1
(0.1) 		29.5
(-3) 		30.4
(-2.4) 		38.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 28.5
(-2.1)
P 		34.3
(-0.8)
P 		24
(-3)
P 		36.8
(-0.3)
P 		52.5
(3)
P 		47.4
(1.9)
P 		29
B 38.4
(-2.8)
P 		36.2
(-3)
P 		38.8
(-2.7)
P 		45.3
(-2.1)
P 		73.8
(0.7)
P 		97.1
(3)
P 		61
C 31.2
(-2.7)
P 		42.2
(-1.4)
P 		35.3
(-2.2)
P 		28.6
(-3)
P 		55
(0.1)
P 		79.9
(3)
P 		51
avr 32.7
(-3) 		37.6
(-2.3) 		32.7
(-3) 		36.9
(-2.4) 		60.4
(0.9) 		74.8
(3) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182952_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7561
Sequence Type Exemplar sequence
Representative Public ID F35G12.5 NCBI
Target Description F35G12.5 /REP_DB=WormBase Gene ID /WP=CE00974 /TR=Q20055 /GB=CAA86331.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065532(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065532 NCBI Caenorhabditis elegans F35G12.5 (F35G12.5) mRNA, complete cds. 11/11 None
GENEFINDER00000042261 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4584568:4586439:1 11/11 None
SNAP00000042241 ENSEMBL cdna:SNAP chromosome:CEL140:III:4585705:4586439:1 9/11 None
F35G12.5 ENSEMBL cdna:known chromosome:CEL140:III:4584897:4586439:1 gene:F35G12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4584896-4586439(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35G12.5
Entrez Gene 175602 Entrez gene
SwissProt Q20055 EMBL-EBI
WormBase CE00974 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86331 Hypothetical protein F35G12.5 [Caenorhabditis elegans] ref|NP_497933.1| F35G12.5 [Caenorhabditis elegans] 0.0
blast CAA86331 Hypothetical protein F35G12.5 [Caenorhabditis elegans] ref|NP_497933.1| F35G12.5 [Caenorhabditis elegans] 1.0E-102
blast CAE71179 Hypothetical protein CBG18036 [Caenorhabditis briggsae] 8.0E-34
blast CAE71179 Hypothetical protein CBG18036 [Caenorhabditis briggsae] 7.0E-33
blast CAE71179 Hypothetical protein CBG18036 [Caenorhabditis briggsae] 3.0E-27
Sequence
Target Sequence 
>C. ELEGANS:182952_AT
gacgactcgctgaaatttggtgggcaaaaaatttgagattttttccgcaaaccatgtacg
tgctgcagaaacttgatcagaaaaatccagatgggtttaattacactgattttgaggaag
aggttcagaaagtgatcccaaggtcctggatacagaaccatcattcatttacactcttgt
cgctgttgaatgaagagagcctttacgtgaagttcaacttgataatgccctcggattctg
agaatatgaacagagtgaaggtcaaccaagtaattaaatttctcggtttccatgaaattg
gagactgtgagttttctatgatctatcaatatgttagttcaaagtccgcttcagaatcta
catacagcatccacaaattcatgtgtgaagactttccgggatgggagacacaatacaata
acgaaaagaaaagcatggataggatctttgagataattattcaggagcaaaaattatttt
ggacggaatatgattatcatacatacaaacgccatgcatacaagattgctgtcccgatgg
tcttctcgcttaacattgtag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGACTCGCTGAAATTTGGTGGGC 461 379 440 Antisense
GATTTTTTCCGCAAACCATGTACGT 134 427 476 Antisense
GTGATCCCAAGGTCCTGGATACAGA 410 483 571 Antisense
GAACCATCATTCATTTACACTCTTG 531 345 594 Antisense
TTTACACTCTTGTCGCTGTTGAATG 328 673 607 Antisense
GTTCAACTTGATAATGCCCTCGGAT 80 445 651 Antisense
GACTGTGAGTTTTCTATGATCTATC 118 377 742 Antisense
GTTCAAAGTCCGCTTCAGAATCTAC 470 443 776 Antisense
ATCTACATACAGCATCCACAAATTC 197 31 795 Antisense
ATACAAGATTGCTGTCCCGATGGTC 59 25 957 Antisense
ATGGTCTTCTCGCTTAACATTGTAG 348 51 976 Antisense
Sequence Source Affymetrix Proprietary Database