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[182924_at]

Description C25F6.1 /REP_DB=WormBase Gene ID /WP=CE02494 /TR=Q18166 /GB=AAK39194.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 71      6grp_total > 82      4grp_total > 19      6grp_high1_sub > 02_03_04_05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 204.1
(-1.9)
P 		187.3
(-3)
P 		187.1
(-3)
P 		279.2
(3)
P 		92
B 179.5
(-2.1)
P 		243.4
(3)
P 		168.3
(-3)
P 		223.4
(1.4)
P 		75
C 192.9
(-3)
P 		201.7
(-2.5)
P 		195.5
(-2.8)
P 		290.7
(3)
P 		98
avr 192.2
(-2.4) 		210.8
(-1) 		183.6
(-3) 		264.4
(3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 228.1
(-1.9)
P 		294.5
(0.1)
P 		314.7
(0.7)
P 		390.8
(3)
P 		374.9
(2.5)
P 		190.4
(-3)
P 		200
B 240.9
(-2.3)
P 		319.1
(-0.5)
P 		349
(0.3)
P 		346.2
(0.2)
P 		464.4
(3)
P 		211.8
(-3)
P 		253
C 201
(-3)
P 		425.5
(2)
P 		297
(-0.8)
P 		468.8
(3)
P 		205.1
(-2.9)
P 		251
(-1.9)
P 		268
avr 223.3
(-2.8) 		346.4
(1.2) 		320.2
(0.3) 		401.9
(3) 		348.1
(1.2) 		217.7
(-3) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 74.3
(3)
P 		40.7
(-3)
P 		48.9
(-1.5)
P 		40.9
(-3)
P 		34
B 57.2
(2.2)
P 		60.1
(3)
P 		39
(-3)
P 		47.6
(-0.6)
P 		21
C 63.9
(1.7)
P 		58
(-0.2)
P 		68.1
(3)
P 		49
(-3)
P 		19
avr 65.1
(3) 		52.9
(-0.8) 		52
(-1.1) 		45.8
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 42
(-3)
P 		104.7
(2.3)
P 		68.5
(-0.8)
P 		99.1
(1.8)
P 		113.1
(3)
P 		46.3
(-2.6)
P 		71
B 83.8
(-2.7)
P 		106.2
(-1.5)
P 		84.8
(-2.7)
P 		91.7
(-2.3)
P 		188.7
(3)
P 		79
(-3)
P 		110
C 74.5
(-2.2)
P 		129.8
(3)
P 		75.6
(-2.1)
P 		94.3
(-0.4)
P 		66.4
(-3)
P 		84
(-1.3)
P 		63
avr 66.8
(-3) 		113.6
(2) 		76.3
(-2) 		95
(0) 		122.7
(3) 		69.8
(-2.7) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182924_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16033
Sequence Type Exemplar sequence
Representative Public ID C25F6.1 NCBI
Target Description C25F6.1 /REP_DB=WormBase Gene ID /WP=CE02494 /TR=Q18166 /GB=AAK39194.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076528(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076528 NCBI Caenorhabditis elegans C25F6.1 (C25F6.1) mRNA, complete cds. 11/11 None
SNAP00000000868 ENSEMBL cdna:SNAP chromosome:CEL140:X:5486460:5487656:1 11/11 None
GENEFINDER00000000870 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5486460:5487656:1 11/11 None
C25F6.1 ENSEMBL cdna:known chromosome:CEL140:X:5486460:5488064:1 gene:C25F6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5486459-5487656(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25F6.1
Entrez Gene 180820 Entrez gene
SwissProt Q18166 EMBL-EBI
WormBase CE02494 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39194 Hypothetical protein C25F6.1 [Caenorhabditis elegans] ref|NP_508929.1| C25F6.1 [Caenorhabditis elegans] 2.0E-96
blast CAE68758 Hypothetical protein CBG14692 [Caenorhabditis briggsae] 1.0E-36
Sequence
Target Sequence 
>C. ELEGANS:182924_AT
tttatcctccactggttcacgatgatgacgtgctcgcacattccgccacagagtcggaag
gaagtgacgaagaagaggatgatttgacaaagcgattgaaagtgactttggcattttcaa
cagacatgatgtcagaatgccgcacagcctgggaagactcaacttgtaaaccaccgattc
agtatctcgaggacaatattcaaaaagatgttgaatatatactcgagaaactggtcaatc
tgaaaatcaagcccaacaatccaattgaatggcttggatactgtcttttgaacatgaaca
tcaatgatcctcgaaaatcaaaagatcacaaaaataaaccatcggaattgttgcgagact
ttaaaatcgaagcgcttgtcaatatggtatggaatatggaggaaggtggctcaggaagca
tttctactttgcacaagcaggcacctagtcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTATCCTCCACTGGTTCACGATGA 303 673 83 Antisense
GATGATGACGTGCTCGCACATTCCG 246 411 103 Antisense
GCACATTCCGCCACAGAGTCGGAAG 695 315 118 Antisense
GTGACTTTGGCATTTTCAACAGACA 178 483 184 Antisense
ATGATGTCAGAATGCCGCACAGCCT 75 47 208 Antisense
CGCACAGCCTGGGAAGACTCAACTT 256 255 223 Antisense
GACTCAACTTGTAAACCACCGATTC 675 369 238 Antisense
ACCACCGATTCAGTATCTCGAGGAC 319 85 252 Antisense
GAATGGCTTGGATACTGTCTTTTGA 200 333 349 Antisense
GGTGGCTCAGGAAGCATTTCTACTT 491 497 487 Antisense
TTTGCACAAGCAGGCACCTAGTCCA 520 667 510 Antisense
Sequence Source Affymetrix Proprietary Database