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[182922_s_at]

Description C04F12.1 /REP_DB=WormBase Gene ID /WP=CE19674 /TR=Q9XVF1 /GB=CAB03833.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_high_8_8 > 11      6grp_highest_nonorm > 99      6grp_total > 88      4grp_total > 14      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 406
1 2 3 4 range
A 394.8
(0.9)
P 		199.6
(-3)
P 		288
(-1.2)
M 		497.5
(3)
P 		298
B 403.2
(1)
P 		181.7
(-2.2)
P 		129.9
(-3)
P 		535.4
(3)
P 		406
C 370.9
(0.9)
P 		214.6
(-2.2)
P 		172.7
(-3)
P 		479.9
(3)
P 		307
avr 389.6
(0.8) 		198.6
(-3) 		196.9
(-3) 		504.3
(3) 		307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1922
1 2 3 4 5 6 range
A 336.8
(-3)
P 		543.5
(-2.3)
P 		374.1
(-2.9)
P 		534
(-2.4)
P 		1210.5
(-0.2)
P 		2230.9
(3)
P 		1894
B 308.5
(-3)
P 		327.7
(-2.9)
P 		400.3
(-2.6)
P 		471.1
(-2.4)
P 		1145.5
(0.3)
P 		1816.3
(3)
P 		1508
C 360.6
(-3)
P 		394.7
(-2.9)
P 		581.6
(-2.2)
P 		366.5
(-3)
P 		1383.5
(0.9)
P 		1926.4
(3)
P 		1566
avr 335.3
(-3) 		422
(-2.7) 		452
(-2.6) 		457.2
(-2.6) 		1246.5
(0.3) 		1991.2
(3) 		1656
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 143.7
(3)
P 		43.3
(-3)
P 		75.3
(-1.1)
M 		72.8
(-1.2)
P 		100
B 128.6
(3)
P 		44.9
(-2.1)
P 		30.1
(-3)
P 		114.1
(2.1)
P 		99
C 122.8
(3)
P 		61.7
(-2.9)
P 		60.2
(-3)
P 		80.9
(-1)
P 		63
avr 131.7
(3) 		50
(-3) 		55.2
(-2.6) 		89.3
(-0.1) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 615
1 2 3 4 5 6 range
A 62.1
(-3)
P 		193.3
(-1.4)
P 		81.5
(-2.8)
P 		135.4
(-2.1)
P 		365.3
(0.8)
P 		543.1
(3)
P 		481
B 107.4
(-2.9)
P 		109.1
(-2.9)
P 		97.2
(-3)
P 		124.8
(-2.7)
P 		465.4
(0.8)
P 		677.3
(3)
P 		580
C 133.6
(-2.4)
P 		120.4
(-2.5)
P 		148
(-2.2)
P 		73.7
(-3)
P 		447.7
(0.9)
P 		644.9
(3)
P 		571
avr 101
(-3) 		140.9
(-2.5) 		108.9
(-2.9) 		111.3
(-2.9) 		426.1
(0.7) 		621.8
(3) 		521
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182922_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1608
Sequence Type Exemplar sequence
Representative Public ID C04F12.1 NCBI
Target Description C04F12.1 /REP_DB=WormBase Gene ID /WP=CE19674 /TR=Q9XVF1 /GB=CAB03833.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060172(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060172 NCBI Caenorhabditis elegans C04F12.1 (C04F12.1) mRNA, complete cds. 11/11 None
SNAP00000037397 ENSEMBL cdna:SNAP chromosome:CEL140:I:9681979:9686619:-1 11/11 None
GENEFINDER00000037413 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9681979:9686619:-1 11/11 None
C04F12.1 ENSEMBL cdna:known chromosome:CEL140:I:9681818:9686674:-1 gene:C04F12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9681909-9686550(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C04F12.1
Entrez Gene 172816 Entrez gene
SwissProt Q9XVF1 EMBL-EBI
WormBase CE19674 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03833 Hypothetical protein C04F12.1 [Caenorhabditis elegans] ref|NP_492573.1| C04F12.1 [Caenorhabditis elegans] 0.0
blast CAE60153 Hypothetical protein CBG03703 [Caenorhabditis briggsae] 0.0
blast CAB54247 Hypothetical protein ZK757.3b [Caenorhabditis elegans] emb|CAB54514.1| Hypothetical protein ZK757.3b [Caenorhabditis elegans] ref|NP_499192.1| Temporarily Assigned Gene name family member (tag-76) [Caenorhabditis elegans] 3.0E-28
blast AAM11104 GM07030p [Drosophila melanogaster] 9.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 6.2E-34
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 6.2E-34
Sequence
Target Sequence 
>C. ELEGANS:182922_S_AT
tggtcattttgcgcgacggagtctctgaaggacaacacaaaatggtcagaaacgaagagt
tccaggcgattaaacagagcatcatgaatgttttcaatgaaaagaaggcaaaggctccgt
cttttgctcttctcgttgtcactaagcgtcatgccaatcggcttttgattaaagatcaga
atggatatacgaatgtcccaccgttgacggcaattgaccaaacaattgtgaagaagcaag
gaaatgaagtcatctttgtgtcccattgtccactcaatggtaccgcccaaccaattgtaa
tcaatacattggtcaacgatggaattttcaagaccaatgatcaattggtccagttcatca
cagcactttgctgtgctcatcagaagtctacaaacatagtgtctctcccagagagtatct
acgcagctgacgaatacgccaaaagaggagccgatttgttccagtcgtataaactgaagc
atcgagatcttccaacgactacgatcgacgactcgacccagctcgactacgaaaaaatca
ccctaattctctgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTCATTTTGCGCGACGGAGTCTC 675 533 2255 Antisense
AAGGCAAAGGCTCCGTCTTTTGCTC 292 161 2359 Antisense
TCTCGTTGTCACTAAGCGTCATGCC 254 611 2385 Antisense
GAATGTCCCACCGTTGACGGCAATT 42 331 2445 Antisense
ATGAAGTCATCTTTGTGTCCCATTG 13 45 2498 Antisense
GTACCGCCCAACCAATTGTAATCAA 524 457 2534 Antisense
TCAATTGGTCCAGTTCATCACAGCA 482 633 2595 Antisense
TCTACGCAGCTGACGAATACGCCAA 302 615 2672 Antisense
AGAGGAGCCGATTTGTTCCAGTCGT 202 67 2698 Antisense
CAACGACTACGATCGACGACTCGAC 703 187 2747 Antisense
AAAAAATCACCCTAATTCTCTGCTT 58 131 2786 Antisense
Sequence Source Affymetrix Proprietary Database