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[182889_at]

Description C50E3.5 /REP_DB=WormBase Gene ID /WP=CE08898 /TR=Q22924 /GB=AAB04879.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 55      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 166.3
(3)
A 		159.9
(2.4)
A 		98.6
(-3)
A 		139.8
(0.7)
A 		68
B 218.1
(3)
A 		119.1
(-3)
A 		162
(-0.4)
A 		146.3
(-1.4)
A 		99
C 197.9
(3)
A 		104.7
(-1.6)
A 		149.6
(0.6)
A 		76.1
(-3)
A 		122
avr 194.1
(3) 		127.9
(-2.4) 		136.7
(-1.7) 		120.7
(-3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 146.8
(-3)
A 		156
(-2.5)
A 		159.4
(-2.3)
M 		206.7
(0.3)
A 		255.6
(3)
M 		156.9
(-2.4)
P 		109
B 115.3
(-3)
A 		130.7
(-2.1)
A 		184.4
(1)
A 		132.5
(-2)
A 		218.2
(3)
P 		212.9
(2.7)
P 		103
C 133.5
(-1.6)
A 		111.4
(-3)
A 		150.6
(-0.5)
A 		157
(-0.1)
A 		177.6
(1.2)
A 		206.9
(3)
P 		96
avr 131.9
(-3) 		132.7
(-2.9) 		164.8
(-0.7) 		165.4
(-0.6) 		217.1
(3) 		192.2
(1.2) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 60.6
(3)
A 		34.7
(-0.9)
A 		25.8
(-2.2)
A 		20.5
(-3)
A 		40
B 69.6
(3)
A 		29.4
(-3)
A 		37.5
(-1.8)
A 		31.2
(-2.7)
A 		40
C 65.5
(3)
A 		30.1
(-1)
A 		52.1
(1.5)
A 		12.8
(-3)
A 		53
avr 65.2
(3) 		31.4
(-1.6) 		38.5
(-0.7) 		21.5
(-3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 27.1
(-3)
A 		55.5
(0.4)
A 		34.7
(-2.1)
M 		52.4
(0)
A 		77.1
(3)
M 		38.2
(-1.7)
P 		50
B 40.1
(-2.4)
A 		43.5
(-2.1)
A 		44.8
(-1.9)
A 		35.1
(-3)
A 		88.6
(3)
P 		79.4
(2)
P 		54
C 49.5
(-0.2)
A 		34
(-2.6)
A 		38.3
(-1.9)
A 		31.6
(-3)
A 		57.5
(1.1)
A 		69.3
(3)
P 		38
avr 38.9
(-3) 		44.3
(-2.1) 		39.3
(-2.9) 		39.7
(-2.9) 		74.4
(3) 		62.3
(1) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182889_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12459
Sequence Type Exemplar sequence
Representative Public ID C50E3.5 NCBI
Target Description C50E3.5 /REP_DB=WormBase Gene ID /WP=CE08898 /TR=Q22924 /GB=AAB04879.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072556(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072556 NCBI Caenorhabditis elegans C50E3.5 (C50E3.5) mRNA, complete cds. 10/11 A 
SNAP00000028527 ENSEMBL cdna:SNAP chromosome:CEL140:V:7590590:7591640:1 11/11 None
GENEFINDER00000028556 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7590590:7591122:1 10/11 None
C50E3.5 ENSEMBL cdna:known chromosome:CEL140:V:7590588:7597330:1 gene:C50E3.5 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7590380-7591641(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50E3.5
Entrez Gene 183656 Entrez gene
SwissProt Q22924 EMBL-EBI
WormBase CE30738 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504957 C50E3.5 [Caenorhabditis elegans] gb|AAB04881.2| Hypothetical protein C50E3.5 [Caenorhabditis elegans] 0.0
blast CAE74650 Hypothetical protein CBG22447 [Caenorhabditis briggsae] 1.0E-107
blast T29670 hypothetical protein C50E3.5 - Caenorhabditis elegans 1.0E-78
blast NP_504957 C50E3.5 [Caenorhabditis elegans] gb|AAB04881.2| Hypothetical protein C50E3.5 [Caenorhabditis elegans] 5.0E-69
blast NP_504957 C50E3.5 [Caenorhabditis elegans] gb|AAB04881.2| Hypothetical protein C50E3.5 [Caenorhabditis elegans] 1.0E-68
blast T29670 hypothetical protein C50E3.5 - Caenorhabditis elegans 4.0E-54
blast T29670 hypothetical protein C50E3.5 - Caenorhabditis elegans 3.0E-53
Sequence
Target Sequence 
>C. ELEGANS:182889_AT
cgaaaaaatcctacatgcacctctcgtgagctctcaagtatttgatcccgatgtctggtc
gtggaattctgctccagaatcgtccctggatttacccatcgattcttctccaaatttctc
gatgccccccgcaacatcttccgagacagaattcgcagaaaacgaagaatcaactagctc
gtcagacgataaaaagaagcgttccgaagtaatcgaagaaaatcgatccgacggacatgc
tcccatcaaatcagcactgtcaattctgatgcgatcacttcaaaaatcatcgtcaatgac
aacctcactatcggatattgatgtcacacttttcaattttcattccacaaaattcgccga
acgtcttctgaatcgcaacgtgtcaagtgtggtaatcgtggaatcatctcatgttttctc
gaaaaatgcaaagcttaccgtgtcatatagacgttgtcatcagagtttcgagaaaatgat
gccacgttgtgatgcaagtgatccgacgatctatctcctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAAAAAATCCTACATGCACCTCTC 114 253 201 Antisense
CACCTCTCGTGAGCTCTCAAGTATT 634 201 218 Antisense
GATGTCTGGTCGTGGAATTCTGCTC 160 409 250 Antisense
CGATTCTTCTCCAAATTTCTCGATG 686 247 300 Antisense
GAAGAATCAACTAGCTCGTCAGACG 286 341 364 Antisense
AAATCGATCCGACGGACATGCTCCC 194 119 420 Antisense
GACATGCTCCCATCAAATCAGCACT 514 369 434 Antisense
GTCAATGACAACCTCACTATCGGAT 646 463 492 Antisense
AAAATTCGCCGAACGTCTTCTGAAT 105 135 549 Antisense
GTGGAATCATCTCATGTTTTCTCGA 298 491 598 Antisense
AAGTGATCCGACGATCTATCTCCTC 366 159 696 Antisense
Sequence Source Affymetrix Proprietary Database