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[182878_at]

Description T06D4.3 /REP_DB=WormBase Gene ID /WP=CE13305 /TR=O16651 /GB=AAB66125.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 01      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 90.3
(0.5)
A 		8.1
(-3)
A 		48.3
(-1.3)
A 		147.1
(3)
A 		139
B 52.1
(-3)
A 		71.4
(0.7)
A 		83.8
(3)
A 		60.4
(-1.4)
A 		32
C 62.6
(-3)
A 		104
(3)
A 		74.8
(-1.2)
A 		95.7
(1.8)
P 		41
avr 68.3
(-1.9) 		61.2
(-3) 		69
(-1.8) 		101.1
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 57.7
(1.4)
A 		28.1
(-3)
A 		68.2
(3)
M 		31.6
(-2.5)
A 		60
(1.8)
A 		51.3
(0.5)
A 		40
B 63.3
(3)
A 		33.8
(-1.6)
A 		24.5
(-3)
A 		49.3
(0.8)
A 		32.7
(-1.7)
A 		42.9
(-0.2)
A 		39
C 54.8
(3)
A 		43.3
(1.4)
A 		54.5
(3)
A 		11.5
(-3)
A 		22.7
(-1.4)
A 		53.2
(2.8)
A 		43
avr 58.6
(3) 		35.1
(-2.1) 		49.1
(0.9) 		30.8
(-3) 		38.5
(-1.3) 		49.1
(0.9) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 32.9
(3)
A 		1.8
(-3)
A 		12.6
(-0.9)
A 		21.5
(0.8)
A 		31
B 16.6
(0.4)
A 		17.6
(1.3)
A 		19.4
(3)
A 		12.9
(-3)
A 		7
C 20.7
(-1)
A 		29.9
(3)
A 		26.1
(1.3)
A 		16.1
(-3)
P 		14
avr 23.4
(3) 		16.4
(-3) 		19.4
(-0.4) 		16.8
(-2.7) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 10.6
(-1.5)
A 		10
(-1.8)
A 		14.8
(1)
M 		8
(-3)
A 		18.1
(3)
A 		12.5
(-0.3)
A 		10
B 22
(3)
A 		11.3
(-1)
A 		5.9
(-3)
A 		13
(-0.4)
A 		13.3
(-0.2)
A 		16
(0.8)
A 		16
C 20.3
(3)
A 		13.2
(0.6)
A 		13.9
(0.9)
A 		2.3
(-3)
A 		7.4
(-1.3)
A 		17.8
(2.2)
A 		18
avr 17.6
(3) 		11.5
(-0.7) 		11.5
(-0.7) 		7.8
(-3) 		12.9
(0.1) 		15.4
(1.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182878_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4791
Sequence Type Exemplar sequence
Representative Public ID T06D4.3 NCBI
Target Description T06D4.3 /REP_DB=WormBase Gene ID /WP=CE13305 /TR=O16651 /GB=AAB66125.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062128
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
T06D4.3 ENSEMBL cdna:known chromosome:CEL140:II:3388275:3391581:-1 gene:T06D4.3 None
NM_062128 NCBI Caenorhabditis elegans T06D4.3 (T06D4.3) mRNA, complete cds. None
GENEFINDER00000039462 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3387949:3395528:-1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039457 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3388273-3391580(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T06D4.3
Entrez Gene 188175 Entrez gene
SwissProt O16651 EMBL-EBI
WormBase CE36012 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66125 Hypothetical protein T06D4.3 [Caenorhabditis elegans] ref|NP_494529.2| T06D4.3 [Caenorhabditis elegans] 0.0
blast AAB66123 Hypothetical protein T06D4.4 [Caenorhabditis elegans] ref|NP_494530.1| T06D4.4 [Caenorhabditis elegans] 0.0
blast AAB66077 Hypothetical protein F18A12.5 [Caenorhabditis elegans] ref|NP_494531.2| F18A12.5 [Caenorhabditis elegans] 0.0
blast AAB66080 Hypothetical protein F18A12.1 [Caenorhabditis elegans] ref|NP_494537.1| F18A12.1 [Caenorhabditis elegans] 1.0E-139
blast AAL02468 Hypothetical protein C49D10.10 [Caenorhabditis elegans] ref|NP_494679.1| C49D10.10 [Caenorhabditis elegans] 1.0E-131
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 8.9E-10
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.3E-67
Pfam IPR008753 EMBL-EBI Peptidase M13 5.3E-97
Pfam IPR008753 EMBL-EBI Peptidase M13 8.9E-10
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.7E-7
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.4E-12
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.8E-64
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494529.2 1 13-35
T06D4.3 1 13-35
GENEFINDER00000039462 1 185-204
Sequence
Target Sequence 
>C. ELEGANS:182878_AT
gatgtgtcggctgctttagatctagcaatgttcctctttggaagcagcagacatctcata
ggatcctcatttcagaaatgtttgccccgtatatgtccacgcctcattttgactccacct
acccagactatgcaaaactggcgaccattgggatggttatcgggcatgaaatgggccacg
gttttgacacacctgactggctcaaaagtcaaacacgaacttatagcggatggcttcggc
gcagaggtcgcctggaaggcttacaagaagcttgatctttcgaaacagccaagactgatt
ggttttcaagatttcaatgttgataagctctttttctacattcgcgcactggttctcaaa
attgttttttttttcagtacatcaatgttttttcagaaattttttgggaacgagatcccc
gaaaagcaaaaaccgcggagattggagaaggcaactcatcccactcatagtttccgtatc
aat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTGTCGGCTGCTTTAGATCTAG 144 409 1636 Antisense
GATCTAGCAATGTTCCTCTTTGGAA 548 419 1654 Antisense
GCAGCAGACATCTCATAGGATCCTC 596 313 1679 Antisense
CAGAAATGTTTGCCCCGTATATGTC 391 205 1708 Antisense
GTATATGTCCACGCCTCATTTTGAC 572 453 1724 Antisense
CACGGTTTTGACACACCTGACTGGC 277 197 1812 Antisense
TATAGCGGATGGCTTCGGCGCAGAG 274 661 1857 Antisense
GGCGCAGAGGTCGCCTGGAAGGCTT 293 539 1873 Antisense
TAAGCTCTTTTTCTACATTCGCGCA 25 639 1959 Antisense
TTCGCGCACTGGTTCTCAAAATTGT 320 697 1976 Antisense
TCCCACTCATAGTTTCCGTATCAAT 405 597 2094 Antisense
Sequence Source Affymetrix Proprietary Database