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[182877_at]

Description ZK185.3 /REP_DB=WormBase Gene ID /WP=CE17312 /TR=O44195 /GB=AAB88560.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 21      4grp_high_8_8 > 53      6grp_highest_nonorm > 80      6grp_total > 32      4grp_total > 59      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 614
1 2 3 4 range
A 368.2
(1)
P 		526.3
(3)
P 		511.9
(2.8)
P 		53.2
(-3)
A 		473
B 445.4
(0.7)
P 		663.2
(3)
P 		609.3
(2.4)
P 		89.1
(-3)
A 		574
C 448
(0.4)
P 		667.1
(3)
P 		662.7
(2.9)
P 		164
(-3)
P 		503
avr 420.5
(0.7) 		618.9
(3) 		594.6
(2.7) 		102.1
(-3) 		517

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 369
1 2 3 4 5 6 range
A 318
(1.7)
P 		296.7
(1.3)
P 		394.2
(3)
P 		220.3
(-0)
P 		130.7
(-1.6)
P 		50.6
(-3)
P 		344
B 344.8
(1.6)
P 		272.5
(0.3)
P 		419.7
(3)
P 		274
(0.4)
P 		176.7
(-1.4)
P 		89
(-3)
P 		331
C 247.1
(1.1)
P 		267.1
(1.7)
P 		314.3
(3)
P 		208.5
(-0)
P 		102.9
(-3)
P 		112.9
(-2.7)
P 		211
avr 303.3
(1.5) 		278.8
(1) 		376.1
(3) 		234.3
(0.1) 		136.8
(-1.9) 		84.2
(-3) 		292
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 134.1
(3)
P 		114.2
(2.1)
P 		133.8
(3)
P 		7.8
(-3)
A 		126
B 142
(2.1)
P 		163.8
(3)
P 		141.2
(2.1)
P 		19
(-3)
A 		145
C 148.3
(0.6)
P 		191.8
(1.8)
P 		231
(3)
P 		27.7
(-3)
P 		203
avr 141.5
(1.9) 		156.6
(2.5) 		168.7
(3) 		18.2
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 58.6
(-0)
P 		105.5
(3)
P 		85.9
(1.7)
P 		55.9
(-0.2)
P 		39.5
(-1.2)
P 		12.3
(-3)
P 		93
B 120
(3)
P 		90.7
(1)
P 		101.9
(1.7)
P 		72.6
(-0.3)
P 		71.8
(-0.3)
P 		33.2
(-3)
P 		87
C 91.6
(3)
P 		81.5
(2)
P 		80
(1.8)
P 		41.9
(-2.1)
P 		33.3
(-3)
P 		37.8
(-2.5)
P 		58
avr 90.1
(2.8) 		92.6
(3) 		89.3
(2.7) 		56.8
(-0.3) 		48.2
(-1.1) 		27.8
(-3) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182877_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10435
Sequence Type Exemplar sequence
Representative Public ID ZK185.3 NCBI
Target Description ZK185.3 /REP_DB=WormBase Gene ID /WP=CE17312 /TR=O44195 /GB=AAB88560.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068184(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068184 NCBI Caenorhabditis elegans ZK185.3 (ZK185.3) mRNA, complete cds. 10/11 None
SNAP00000040942 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4528830:4530160:-1 10/11 None
ZK185.3 ENSEMBL cdna:known chromosome:CEL140:IV:4528830:4530320:-1 gene:ZK185.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040947 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4528835-4530166(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK185.3
Entrez Gene 177220 Entrez gene
SwissProt O44195 EMBL-EBI
WormBase CE31748 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB88560 Hypothetical protein ZK185.3 [Caenorhabditis elegans] ref|NP_500585.2| ZK185.3 [Caenorhabditis elegans] 1.0E-172
blast AAB88560 Hypothetical protein ZK185.3 [Caenorhabditis elegans] ref|NP_500585.2| ZK185.3 [Caenorhabditis elegans] 1.0E-124
blast CAA96676 Hypothetical protein T10B10.4a [Caenorhabditis elegans] ref|NP_001024902.1| T10B10.4a [Caenorhabditis elegans] 6.0E-19
blast CAA96676 Hypothetical protein T10B10.4a [Caenorhabditis elegans] ref|NP_001024902.1| T10B10.4a [Caenorhabditis elegans] 1.0E-17
blast CAE63285 Hypothetical protein CBG07665 [Caenorhabditis briggsae] 2.0E-17
blast CAE63285 Hypothetical protein CBG07665 [Caenorhabditis briggsae] 3.0E-17
Sequence
Target Sequence 
>C. ELEGANS:182877_AT
gcaaagtacttctattgcttccgccataatcgccttccagatagttgccaacaagtatct
tacaacctgctgtaatatccgtgagaactataatgttccctgtaacaacttctacctacc
tatcactgtttgccctgaaattcaagaaaaagtcgatggaatcactcttcctgtctcctt
ttcaattggttccactcgactcgaagatgccgaaatcgtgaattccacgtggaaatttgc
caccccggaagacattttgcaacaaaaagaagggatcacacctgtcaaatcggtggagtt
gagcaatgagccggtactgaaaagaagtttagagcatccactctgggaaatcgtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAAGTACTTCTATTGCTTCCGCC 102 319 15 Antisense
TAATCGCCTTCCAGATAGTTGCCAA 92 637 41 Antisense
GCCAACAAGTATCTTACAACCTGCT 635 277 61 Antisense
ACAACCTGCTGTAATATCCGTGAGA 312 113 76 Antisense
ATGTTCCCTGTAACAACTTCTACCT 674 45 107 Antisense
TGTAACAACTTCTACCTACCTATCA 411 553 115 Antisense
CCTATCACTGTTTGCCCTGAAATTC 195 269 133 Antisense
GTCGATGGAATCACTCTTCCTGTCT 204 473 166 Antisense
TCCTGTCTCCTTTTCAATTGGTTCC 626 593 183 Antisense
GGTTCCACTCGACTCGAAGATGCCG 34 507 202 Antisense
GAGCATCCACTCTGGGAAATCGTGA 434 389 346 Antisense
Sequence Source Affymetrix Proprietary Database