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[182861_s_at] G-protein coupled receptor, srh family

Description F08E10.5 /REP_DB=WormBase Gene ID /WP=CE18575 /TR=Q9XXP0 /GB=CAA16504.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 08      6grp_cgc > 44      6grp_cgc_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 5.8
(-3)
A 		12.5
(-1.6)
A 		16.1
(-0.9)
A 		35.1
(3)
A 		29
B 7.1
(-0)
A 		6.9
(-0.2)
A 		4.4
(-3)
A 		9.8
(3)
A 		5
C 15.8
(-0.9)
A 		6.7
(-3)
A 		29.3
(2.1)
A 		33.2
(3)
A 		27
avr 9.6
(-2.7) 		8.7
(-3) 		16.6
(-0.3) 		26
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 29.6
(3)
A 		3.6
(-2.7)
A 		4.2
(-2.5)
A 		2.9
(-2.8)
A 		18.5
(0.6)
A 		2.1
(-3)
A 		28
B 6.4
(-2.6)
A 		15.8
(-0.7)
A 		34.1
(3)
A 		17.5
(-0.4)
A 		4.5
(-3)
A 		18.2
(-0.2)
A 		30
C 5.2
(-3)
A 		28.1
(3)
A 		8.8
(-2.1)
A 		10.1
(-1.7)
A 		7.8
(-2.3)
A 		5.6
(-2.9)
A 		23
avr 13.7
(1.3) 		15.8
(3) 		15.7
(2.9) 		10.2
(-1.7) 		10.3
(-1.6) 		8.6
(-3) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 2.1
(-3)
A 		2.7
(-1.8)
A 		4.2
(1.2)
A 		5.1
(3)
A 		3
B 2.3
(3)
A 		1.7
(0.2)
A 		1
(-3)
A 		2.1
(2.1)
A 		1
C 5.2
(-0.6)
A 		1.9
(-3)
A 		10.2
(3)
A 		5.6
(-0.3)
A 		8
avr 3.2
(-0.8) 		2.1
(-3) 		5.1
(3) 		4.3
(1.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 5.5
(2.9)
A 		1.3
(-2.1)
A 		0.9
(-2.5)
A 		0.7
(-2.8)
A 		5.6
(3)
A 		0.5
(-3)
A 		5
B 2.2
(-2.6)
A 		5.3
(0.2)
A 		8.3
(3)
A 		4.6
(-0.4)
A 		1.8
(-3)
A 		6.8
(1.6)
A 		7
C 1.9
(-3)
A 		8.6
(3)
A 		2.2
(-2.7)
A 		2
(-2.9)
A 		2.5
(-2.5)
A 		1.9
(-3)
A 		7
avr 3.2
(-1.2) 		5.1
(3) 		3.8
(0.1) 		2.4
(-3) 		3.3
(-1) 		3.1
(-1.4) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182861_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12690
Sequence Type Exemplar sequence
Representative Public ID F08E10.5 NCBI
Target Description F08E10.5 /REP_DB=WormBase Gene ID /WP=CE18575 /TR=Q9XXP0 /GB=CAA16504.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074961(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074961 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-252) (srh-252) mRNA, complete cds. 11/11 A 
SNAP00000010311 ENSEMBL cdna:SNAP chromosome:CEL140:V:17224757:17225982:-1 11/11 None
GENEFINDER00000010323 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17224200:17226519:-1 11/11 None
T19C9.3 ENSEMBL cdna:known chromosome:CEL140:V:17224963:17225982:-1 gene:T19C9.3 11/11 A 
F08E10.5 ENSEMBL cdna:pseudogene chromosome:CEL140:V:17483427:17484431:-1 gene:F08E10.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17483426-17484431(-) 100.0 100.0
chrV:17224946-17225975(-) 93.03 93.03
Public Domain and Genome References
Gene Title G-protein coupled receptor, srh family
Gene Symbol srh-252
Ensembl T19C9.3
F08E10.5
Entrez Gene 188598 Entrez gene
SwissProt O45794 EMBL-EBI
WormBase CE16420 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07488 Hypothetical protein T19C9.3 [Caenorhabditis elegans] ref|NP_507362.1| Serpentine Receptor, class H family member (srh-252) [Caenorhabditis elegans] 1.0E-171
blast CAB07488 Hypothetical protein T19C9.3 [Caenorhabditis elegans] ref|NP_507362.1| Serpentine Receptor, class H family member (srh-252) [Caenorhabditis elegans] 1.0E-165
blast T20585 hypothetical protein F08E10.5 - Caenorhabditis elegans 1.0E-149
blast T20585 hypothetical protein F08E10.5 - Caenorhabditis elegans 1.0E-146
blast CAB04630 Hypothetical protein R08H2.4 [Caenorhabditis elegans] ref|NP_506819.1| Serpentine Receptor, class H family member (srh-251) [Caenorhabditis elegans] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507362.1 7 15-37,50-72,87-109,130-152,187-209,230-252,267-289
T19C9.3 7 15-37,50-72,87-109,130-152,187-209,230-252,267-289
SNAP00000010311 7 15-37,50-72,87-109,130-152,187-209,230-252,267-289
GENEFINDER00000010323 7 37-59,80-102,112-134,155-177,212-234,255-277,292-314
Sequence
Target Sequence 
>C. ELEGANS:182861_S_AT
accactgatgtacatattcccgctcaccttcacactgccaatctgggcagttatgccaat
acaggaaaacgctcgcccatgcgatgaagctttacaactagaaaatattcacatggaatc
gaagaaaatattcattatatccgtcgatccaataatttcagtcacttggggtgtgactaa
ttgtatactcatcgttacacctatcataacgttcttacaacttctcgaaaaccaaaaaac
acacaaatactcctaccaaacaattcaacttcagaagactttcttattgtccatgtcagt
acaattcggctcttttttcgttatcatttttattcctgtgcttcttttgcacggctccgt
catattctggtatcatgatcaagttcttaacaactttatcacaatcatgttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCACTGATGTACATATTCCCGCTC 403 85 387 Antisense
CTTCACACTGCCAATCTGGGCAGTT 663 221 414 Antisense
GCCAATCTGGGCAGTTATGCCAATA 76 279 423 Antisense
GGCAGTTATGCCAATACAGGAAAAC 128 535 432 Antisense
ATATTCATTATATCCGTCGATCCAA 424 17 514 Antisense
ATCCGTCGATCCAATAATTTCAGTC 614 37 525 Antisense
CAATAATTTCAGTCACTTGGGGTGT 68 179 536 Antisense
TTGGGGTGTGACTAATTGTATACTC 503 697 552 Antisense
GTATACTCATCGTTACACCTATCAT 99 455 569 Antisense
TACTCATCGTTACACCTATCATAAC 587 645 572 Antisense
ACAACTTTATCACAATCATGTTTTC 554 111 776 Antisense
Sequence Source Affymetrix Proprietary Database