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[182856_at]

Description F40C5.3 /REP_DB=WormBase Gene ID /WP=CE20767 /TR=Q9N5U2 /GB=AAF39799.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 57      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 14.8
(-3)
A 		45.9
(-1.9)
A 		177.1
(3)
A 		18.8
(-2.9)
A 		162
B 9
(-3)
A 		102.3
(3)
A 		69.6
(0.9)
A 		8.4
(-3)
A 		94
C 36.9
(-1.9)
A 		146.6
(3)
A 		110.9
(1.4)
A 		13.4
(-3)
A 		133
avr 20.2
(-2.6) 		98.3
(1.8) 		119.2
(3) 		13.5
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 19.4
(-3)
A 		53.6
(-0.5)
A 		69.3
(0.7)
A 		27.3
(-2.4)
A 		59.7
(-0)
A 		100.6
(3)
A 		81
B 43.9
(-2.4)
A 		100.9
(0.4)
A 		46.6
(-2.3)
A 		32.6
(-3)
A 		153.1
(3)
A 		112.2
(1)
A 		121
C 24.6
(-3)
A 		42.6
(-1.6)
A 		33.8
(-2.3)
A 		75.6
(0.9)
A 		103.7
(3)
A 		78.6
(1.1)
A 		79
avr 29.3
(-3) 		65.7
(-0.1) 		49.9
(-1.4) 		45.2
(-1.7) 		105.5
(3) 		97.1
(2.3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 5.4
(-2.6)
A 		10
(-2)
A 		46.3
(3)
A 		2.7
(-3)
A 		44
B 2.9
(-2.7)
A 		25.3
(3)
A 		16.1
(0.7)
A 		1.8
(-3)
A 		24
C 12.2
(-1.5)
A 		42.2
(3)
A 		38.7
(2.5)
A 		2.3
(-3)
A 		40
avr 6.8
(-2.1) 		25.8
(1.5) 		33.7
(3) 		2.3
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 3.6
(-3)
A 		19.1
(1.4)
A 		15.1
(0.3)
A 		6.9
(-2.1)
A 		18
(1.1)
A 		24.5
(3)
A 		21
B 15.3
(-2.3)
A 		33.6
(-0.2)
A 		11.3
(-2.7)
A 		8.6
(-3)
A 		62.2
(3)
A 		41.8
(0.7)
A 		54
C 9.1
(-2.9)
A 		13
(-1.9)
A 		8.6
(-3)
A 		15.2
(-1.4)
A 		33.6
(3)
A 		26.3
(1.2)
A 		25
avr 9.3
(-3) 		21.9
(-0.4) 		11.7
(-2.5) 		10.2
(-2.8) 		37.9
(3) 		30.9
(1.5) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182856_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15672
Sequence Type Exemplar sequence
Representative Public ID F40C5.3 NCBI
Target Description F40C5.3 /REP_DB=WormBase Gene ID /WP=CE20767 /TR=Q9N5U2 /GB=AAF39799.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071605 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-27) (grl-27) mRNA, complete cds. 11/11 None
SNAP00000026432 ENSEMBL cdna:SNAP chromosome:CEL140:V:3574944:3575677:-1 11/11 None
GENEFINDER00000026435 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3574944:3575677:-1 11/11 None
F40C5.3 ENSEMBL cdna:known chromosome:CEL140:V:3574944:3575677:-1 gene:F40C5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000027446 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3574943-3575677(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-27
Ensembl F40C5.3
Entrez Gene 185516 Entrez gene
SwissProt Q9N5U2 EMBL-EBI
WormBase CE20767 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39799 Ground-like (grd related) protein 27 [Caenorhabditis elegans] ref|NP_504006.1| GRound-Like (grd related) family member (grl-27) [Caenorhabditis elegans] 1.0E-48
blast CAE58215 Hypothetical protein CBG01310 [Caenorhabditis briggsae] 3.0E-40
blast AAD34663 Ground-like (grd related) protein 31 [Caenorhabditis elegans] ref|NP_503999.1| GRound-Like (grd related) family member (grl-31) [Caenorhabditis elegans] 5.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 7.7E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504006.1 1 5-27
F40C5.3 1 5-27
SNAP00000026432 1 5-27
GENEFINDER00000026435 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:182856_AT
caggcctcttcggcatgggaggtggaggtgggtgtggatgtggtgcaccgccaccaccac
ctgcctgtggatgtggtggaagaaagaagagatccctgccagaaaaaccagagttcttcg
gaatcgctgcaggagaccaggatgtgatgtgcaacaccccggagctgaagaagatcattc
tggagaatactcaaaccaccgccgtcgactcttccaaggccatcaacaccgttcttgaga
gccgccagcttcaacgatttgtagttgtgtgctcagagaacccgttcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGGCCTCTTCGGCATGGGAGGTGG 542 207 68 Antisense
GTGGAGGTGGGTGTGGATGTGGTGC 700 487 89 Antisense
TGTGGATGTGGTGCACCGCCACCAC 344 555 100 Antisense
ACCACCACCTGCCTGTGGATGTGGT 16 87 120 Antisense
GAGATCCCTGCCAGAAAAACCAGAG 492 395 156 Antisense
AAAACCAGAGTTCTTCGGAATCGCT 689 127 171 Antisense
GAATCGCTGCAGGAGACCAGGATGT 546 329 188 Antisense
TGCAACACCCCGGAGCTGAAGAAGA 538 575 217 Antisense
GGCCATCAACACCGTTCTTGAGAGC 176 543 285 Antisense
CCGCCAGCTTCAACGATTTGTAGTT 178 263 309 Antisense
TTGTGTGCTCAGAGAACCCGTTCGT 681 673 332 Antisense
Sequence Source Affymetrix Proprietary Database