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[182823_at]

Description F20A1.4 /REP_DB=WormBase Gene ID /WP=CE07094 /TR=Q19608 /GB=AAA96124.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      6grp_highest_nonorm > 80      6grp_total > 25      4grp_total > 22      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 225.5
(3)
P 		80
(-2.9)
M 		77.7
(-3)
A 		219.7
(2.8)
P 		148
B 233.4
(1.9)
P 		108.8
(-1.9)
P 		74.1
(-3)
A 		267.4
(3)
P 		193
C 270.4
(2.6)
P 		92.6
(-2.4)
A 		72.1
(-3)
A 		285.2
(3)
P 		213
avr 243.1
(2.5) 		93.8
(-2.4) 		74.6
(-3) 		257.4
(3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 227
1 2 3 4 5 6 range
A 214
(2.8)
P 		217.3
(3)
P 		169.6
(-0.3)
P 		130.1
(-3)
P 		178.2
(0.3)
P 		158.1
(-1.1)
P 		87
B 223.4
(1.9)
P 		243.3
(3)
P 		169.5
(-1.1)
P 		175
(-0.8)
P 		134.8
(-3)
P 		134.4
(-3)
P 		109
C 250.1
(1.1)
P 		320.6
(3)
P 		177.9
(-0.8)
P 		93.5
(-3)
P 		131.2
(-2)
P 		152.5
(-1.4)
P 		227
avr 229.2
(1.5) 		260.4
(3) 		172.3
(-1.1) 		132.9
(-3) 		148.1
(-2.3) 		148.3
(-2.3) 		128
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 82.1
(3)
P 		17.4
(-3)
M 		20.3
(-2.7)
A 		32.2
(-1.6)
P 		65
B 74.4
(3)
P 		26.9
(-2)
P 		17.2
(-3)
A 		57
(1.2)
P 		57
C 89.5
(3)
P 		26.6
(-2.9)
A 		25.1
(-3)
A 		48.1
(-0.9)
P 		64
avr 82
(3) 		23.6
(-2.7) 		20.9
(-3) 		45.8
(-0.6) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 39.4
(-2.1)
P 		77.3
(3)
P 		36.9
(-2.5)
P 		33
(-3)
P 		53.8
(-0.2)
P 		38.5
(-2.3)
P 		44
B 77.7
(2.5)
P 		81
(3)
P 		41.2
(-3)
P 		46.3
(-2.2)
P 		54.8
(-0.9)
P 		50.1
(-1.7)
P 		40
C 92.7
(2.6)
P 		97.8
(3)
P 		45.3
(-1)
P 		18.8
(-3)
P 		42.5
(-1.2)
P 		51
(-0.6)
P 		79
avr 69.9
(1.2) 		85.4
(3) 		41.1
(-2) 		32.7
(-3) 		50.4
(-1) 		46.5
(-1.4) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182823_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12462
Sequence Type Exemplar sequence
Representative Public ID F20A1.4 NCBI
Target Description F20A1.4 /REP_DB=WormBase Gene ID /WP=CE07094 /TR=Q19608 /GB=AAA96124.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072378(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072378 NCBI Caenorhabditis elegans F20A1.4 (F20A1.4) mRNA, complete cds. 11/11 None
SNAP00000026798 ENSEMBL cdna:SNAP chromosome:CEL140:V:7021494:7023415:-1 11/11 None
GENEFINDER00000026810 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7021494:7024362:-1 11/11 None
F20A1.4 ENSEMBL cdna:known chromosome:CEL140:V:7021494:7024396:-1 gene:F20A1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7021494-7022489(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F20A1.4
Entrez Gene 179086 Entrez gene
SwissProt Q5WRR3 EMBL-EBI
WormBase CE37634 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504779 F20A1.4 [Caenorhabditis elegans] gb|AAV28350.1| Hypothetical protein F20A1.4 [Caenorhabditis elegans] 0.0
blast T30034 hypothetical protein F20A1.4 - Caenorhabditis elegans 1.0E-156
blast T30034 hypothetical protein F20A1.4 - Caenorhabditis elegans 1.0E-145
blast CAE72038 Hypothetical protein CBG19120 [Caenorhabditis briggsae] 1.0E-69
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504779.2 13 20-42,62-81,83-102,112-129,156-178,188-210,223-245,260-282,295-317,327-349,358-380,395-417,424-446
F20A1.4 13 20-42,62-81,83-102,112-129,156-178,188-210,223-245,260-282,295-317,327-349,358-380,395-417,424-446
SNAP00000026798 7 25-47,57-79,92-114,129-151,164-186,250-272,279-301
GENEFINDER00000026810 13 20-42,62-81,83-102,112-129,156-178,188-210,223-245,260-282,295-317,327-349,358-380,395-417,424-446
Sequence
Target Sequence 
>C. ELEGANS:182823_AT
gacacggtggacgcattgttttctcaagtaacaatttcctcgatctgttctggatttgca
cttttgtatctttggtatcactttggccagtcaaatgtgattttactggttatcattcta
ttgataaccaattggatcacagtggtcttcttctatccatctgcaatcgccacaattggt
ccgttaatctgcggagggaagcgacgtggaagtcagatatcggagacacgatccatgaat
actgatcgattttcttcgcatcttcttgttgataacccagattacacaaagaaatactcc
acgagtaattactatggatcagctaaacagaagaggtcctataatgttcttgtgaccgat
tctagaagagcttccatgccagtctgtgttcagcaaattgcgattactaactcgaatgcg
cca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACGGTGGACGCATTGTTTTCTC 506 365 337 Antisense
AACAATTTCCTCGATCTGTTCTGGA 373 133 366 Antisense
TCTGTTCTGGATTTGCACTTTTGTA 87 609 380 Antisense
ATCTTTGGTATCACTTTGGCCAGTC 435 31 404 Antisense
GATCACAGTGGTCTTCTTCTATCCA 692 421 471 Antisense
ATCCATCTGCAATCGCCACAATTGG 27 41 491 Antisense
GCCACAATTGGTCCGTTAATCTGCG 181 279 505 Antisense
ACTGATCGATTTTCTTCGCATCTTC 394 97 577 Antisense
ATAATGTTCTTGTGACCGATTCTAG 296 25 677 Antisense
CTAGAAGAGCTTCCATGCCAGTCTG 94 225 698 Antisense
TGCGATTACTAACTCGAATGCGCCA 695 579 735 Antisense
Sequence Source Affymetrix Proprietary Database