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[182813_at]

Description F40D4.12 /REP_DB=WormBase Gene ID /WP=CE19846 /TR=Q9XV32 /GB=CAB04362.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 35      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 28
(-0.4)
A 		54.4
(3)
A 		7.2
(-3)
A 		36.3
(0.7)
A 		47
B 23.5
(-3)
A 		55.5
(3)
M 		41.6
(0.4)
A 		28
(-2.2)
A 		32
C 49.5
(0.6)
A 		52
(0.9)
A 		25.1
(-3)
A 		66.2
(3)
P 		41
avr 33.7
(-1.1) 		54
(3) 		24.6
(-3) 		43.5
(0.9) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 4.9
(-2.8)
A 		31.7
(3)
A 		20.5
(0.6)
A 		4.1
(-3)
A 		11.1
(-1.5)
A 		4.5
(-2.9)
A 		28
B 17.1
(-1.7)
A 		32.4
(0.6)
A 		48.6
(3)
P 		20.1
(-1.2)
A 		17.6
(-1.6)
A 		8.2
(-3)
A 		40
C 25.9
(2)
A 		28.1
(2.4)
A 		30.8
(3)
A 		28.4
(2.5)
A 		1.6
(-3)
A 		3.3
(-2.7)
A 		29
avr 16
(-0.7) 		30.7
(2.4) 		33.3
(3) 		17.5
(-0.4) 		10.1
(-2) 		5.3
(-3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 10.2
(2)
A 		11.8
(3)
A 		1.9
(-3)
A 		5.3
(-0.9)
A 		10
B 7.5
(-1.8)
A 		13.7
(3)
M 		9.6
(-0.2)
A 		6
(-3)
A 		8
C 16.4
(3)
A 		15
(1.9)
A 		8.7
(-3)
A 		11.2
(-1.1)
P 		8
avr 11.4
(1.1) 		13.5
(3) 		6.7
(-3) 		7.5
(-2.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.9
(-3)
A 		11.3
(3)
A 		4.5
(-0.9)
A 		1
(-2.9)
A 		3.3
(-1.6)
A 		1.1
(-2.9)
A 		10
B 5.9
(-1.1)
A 		10.8
(2.3)
A 		11.8
(3)
P 		5.3
(-1.5)
A 		7.2
(-0.2)
A 		3.1
(-3)
A 		9
C 9.6
(3)
A 		8.6
(2.3)
A 		7.8
(1.8)
A 		5.7
(0.4)
A 		0.5
(-3)
A 		1.1
(-2.6)
A 		9
avr 5.5
(-0.4) 		10.2
(3) 		8
(1.4) 		4
(-1.4) 		3.7
(-1.6) 		1.8
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182813_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14669
Sequence Type Exemplar sequence
Representative Public ID F40D4.12 NCBI
Target Description F40D4.12 /REP_DB=WormBase Gene ID /WP=CE19846 /TR=Q9XV32 /GB=CAB04362.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074939(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074939 NCBI Caenorhabditis elegans F40D4.12 (F40D4.12) mRNA, complete cds. 11/11 None
SNAP00000026444 ENSEMBL cdna:SNAP chromosome:CEL140:V:17162452:17165058:1 11/11 None
GENEFINDER00000026456 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17162452:17165058:1 11/11 None
F40D4.12 ENSEMBL cdna:known chromosome:CEL140:V:17162452:17165058:1 gene:F40D4.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17162451-17165058(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F40D4.12
Entrez Gene 185530 Entrez gene
SwissProt Q9XV32 EMBL-EBI
WormBase CE19846 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04362 Hypothetical protein F40D4.12 [Caenorhabditis elegans] ref|NP_507340.1| F40D4.12 [Caenorhabditis elegans] 0.0
blast CAB07255 Hypothetical protein K10G4.5 [Caenorhabditis elegans] ref|NP_507379.2| K10G4.5 [Caenorhabditis elegans] 1.0E-178
blast CAB03900 Hypothetical protein C18D4.2 [Caenorhabditis elegans] ref|NP_507438.1| C18D4.2 [Caenorhabditis elegans] 2.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 6.3E-38
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 0.0018
Sequence
Target Sequence 
>C. ELEGANS:182813_AT
ataatctcaaatctatcggccataaaattcaagtcaaagaactgaacatgcaagcatgca
atcaaaatacagttatgcatgtattgccgtatttggatacacgtgttttgagaagtatat
cgattcaaataccagtccaaaaagagactaaagtcccgtgtgactggattgaaccattag
aaatcaatgaaatctgtgagttggagcaatggaagaatgcgaggatgctagaaatagaac
acgttataaacaccactggcatgaaacatttgggtcacttctccgaagctgatgtgaagc
tgaccagaatgacaaccgaaggcattttgtgtctgaaagctgaattccttctctcgaaca
actttgagaagttcaaggcgtccttcgaaacaagcgatatagacgaaagcatctatgaaa
cagaatatctgggattaccattccggagaccaattaatccgagaacaaatcagccgagca
tttggtatttcaaatatcctggaaccgacgattatcttcacattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAATCTCAAATCTATCGGCCATAA 698 25 1613 Antisense
GTCCCGTGTGACTGGATTGAACCAT 612 475 1765 Antisense
GAACACGTTATAAACACCACTGGCA 555 351 1849 Antisense
GTCACTTCTCCGAAGCTGATGTGAA 421 465 1886 Antisense
GAAGCTGACCAGAATGACAACCGAA 261 343 1908 Antisense
GAAGGCATTTTGTGTCTGAAAGCTG 659 333 1930 Antisense
TGAAAGCTGAATTCCTTCTCTCGAA 234 573 1946 Antisense
TCAAGGCGTCCTTCGAAACAAGCGA 267 631 1985 Antisense
CTGGGATTACCATTCCGGAGACCAA 63 239 2041 Antisense
ATCAGCCGAGCATTTGGTATTTCAA 286 29 2081 Antisense
GGAACCGACGATTATCTTCACATTT 185 531 2113 Antisense
Sequence Source Affymetrix Proprietary Database