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[182803_at]

Description F27C1.3 /REP_DB=WormBase Gene ID /WP=CE09716 /TR=P91282 /GB=AAB37653.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 12.8
(-3)
A 		110.1
(0.9)
A 		67.5
(-0.8)
A 		160.8
(3)
A 		148
B 46.9
(-0.2)
A 		25.4
(-2.1)
A 		15.6
(-3)
A 		82.4
(3)
A 		67
C 56.1
(-0.3)
A 		65.1
(0.4)
A 		25.2
(-3)
A 		94.9
(3)
A 		70
avr 38.6
(-2.8) 		66.9
(-0.6) 		36.1
(-3) 		112.7
(3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 66.4
(-2.9)
A 		65.5
(-3)
A 		76.5
(-2.3)
A 		133.5
(1.1)
A 		155.8
(2.5)
A 		164.7
(3)
M 		99
B 61
(-1.2)
A 		101.4
(0.8)
A 		23.8
(-3)
A 		37.1
(-2.4)
A 		135.5
(2.4)
A 		147
(3)
P 		123
C 136.1
(1.6)
A 		78.3
(-2.4)
A 		103.4
(-0.7)
A 		69.6
(-3)
A 		108.9
(-0.3)
A 		155.9
(3)
P 		86
avr 87.8
(-1.6) 		81.7
(-2.1) 		67.9
(-3) 		80.1
(-2.2) 		133.4
(1.5) 		155.9
(3) 		88
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 4.7
(-3)
A 		23.9
(3)
A 		17.6
(1)
A 		23.5
(2.9)
A 		19
B 15
(1.9)
A 		6.3
(-1.8)
A 		3.6
(-3)
A 		17.6
(3)
A 		14
C 18.6
(2.9)
A 		18.7
(3)
A 		8.8
(-3)
A 		16
(1.4)
A 		10
avr 12.8
(-1.1) 		16.3
(1.2) 		10
(-3) 		19
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 12.2
(-3)
A 		23.3
(-1.1)
A 		16.7
(-2.2)
A 		33.9
(0.7)
A 		47
(3)
A 		40.1
(1.8)
M 		35
B 21.2
(-1.1)
A 		33.7
(0.4)
A 		5.8
(-3)
A 		9.8
(-2.5)
A 		55.1
(3)
A 		54.8
(3)
P 		49
C 50.4
(2.7)
A 		23.9
(-1.4)
A 		26.3
(-1.1)
A 		14
(-3)
A 		35.2
(0.3)
A 		52.2
(3)
P 		38
avr 27.9
(-0.9) 		27
(-1) 		16.3
(-3) 		19.2
(-2.5) 		45.8
(2.4) 		49
(3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182803_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1317
Sequence Type Exemplar sequence
Representative Public ID F27C1.3 NCBI
Target Description F27C1.3 /REP_DB=WormBase Gene ID /WP=CE09716 /TR=P91282 /GB=AAB37653.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059191 NCBI Caenorhabditis elegans F27C1.3 (F27C1.3) mRNA, complete cds. 11/11 None
GENEFINDER00000014528 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5426370:5427200:1 11/11 None
SNAP00000014502 ENSEMBL cdna:SNAP chromosome:CEL140:I:5426451:5427200:1 11/11 None
F27C1.3 ENSEMBL cdna:known chromosome:CEL140:I:5426360:5427319:1 gene:F27C1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5424546-5427200(+) 96.84 100.0
Public Domain and Genome References
Gene Title
Ensembl F27C1.3
Entrez Gene 172194 Entrez gene
SwissProt P91282 EMBL-EBI
WormBase CE28825 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37653 Hypothetical protein F27C1.3 [Caenorhabditis elegans] ref|NP_491592.1| F27C1.3 [Caenorhabditis elegans] 4.0E-53
blast AAB37653 Hypothetical protein F27C1.3 [Caenorhabditis elegans] ref|NP_491592.1| F27C1.3 [Caenorhabditis elegans] 2.0E-52
blast AAB37653 Hypothetical protein F27C1.3 [Caenorhabditis elegans] ref|NP_491592.1| F27C1.3 [Caenorhabditis elegans] 2.0E-45
blast CAE66923 Hypothetical protein CBG12311 [Caenorhabditis briggsae] 9.0E-28
blast CAE66923 Hypothetical protein CBG12311 [Caenorhabditis briggsae] 1.0E-27
blast CAB02815 Hypothetical protein C36F7.5 [Caenorhabditis elegans] ref|NP_492537.1| C36F7.5 [Caenorhabditis elegans] 1.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR004296 EMBL-EBI Protein of unknown function DUF236 2.5E-71
Pfam IPR004296 EMBL-EBI Protein of unknown function DUF236 2.5E-71
Pfam IPR004296 EMBL-EBI Protein of unknown function DUF236 2.5E-71
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491592.1 1 10-32
F27C1.3 1 10-32
GENEFINDER00000014528 1 10-32
Sequence
Target Sequence 
>C. ELEGANS:182803_AT
agctccaaagcctccgaaaaatcgtgctgcaattgctgacacccatgatcccaactacca
gacattggccggattgaacgataacgtttttgagcaaaaacaaggcggtggtggtggtgg
agctgctccaaaagctccggacggaaaaccaggaatggccggaactcatgatcctaacta
tcaaactcttgccggcttgaatgataatgttttcgataagaaaggtggtggtggagcagg
accacaggctccagcagcaggaggtaaacctggaatggctgcaacacacgatccaaacta
tcagactcttgctggtttgaataacaacgtgttcgatcaaaaaggtggtgttggtggagg
tggaggaggaggaaatggtgcaccagctccaccaaaagctcctgctcaacatggtgctaa
ggctgcaactcatgacccgaactaccaaaccctggctggtctcaatcaagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTCCAAAGCCTCCGAAAAATCGT 228 81 456 Antisense
AATCGTGCTGCAATTGCTGACACCC 388 169 475 Antisense
CCATGATCCCAACTACCAGACATTG 590 271 498 Antisense
ATGGCCGGAACTCATGATCCTAACT 102 53 610 Antisense
TAACTATCAAACTCTTGCCGGCTTG 217 623 630 Antisense
ACCACAGGCTCCAGCAGCAGGAGGT 669 83 696 Antisense
GGAATGGCTGCAACACACGATCCAA 442 531 727 Antisense
GAGGAAATGGTGCACCAGCTCCACC 409 405 824 Antisense
AAAGCTCCTGCTCAACATGGTGCTA 23 125 850 Antisense
GTGCTAAGGCTGCAACTCATGACCC 126 479 869 Antisense
AACCCTGGCTGGTCTCAATCAAGAT 287 147 903 Antisense
Sequence Source Affymetrix Proprietary Database