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[182801_at]

Description C13D9.7 /REP_DB=WormBase Gene ID /WP=CE08140 /TR=O16241 /GB=AAB65308.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 44      6grp_cgc > 41      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 239.4
(2.9)
M 		240.7
(3)
A 		186.3
(-0.2)
A 		139
(-3)
A 		102
B 225.9
(0.6)
A 		301
(3)
A 		172.1
(-1.1)
A 		113.5
(-3)
A 		188
C 241.5
(1.8)
A 		260.4
(3)
A 		227.8
(0.9)
A 		168.3
(-3)
A 		92
avr 235.6
(1.5) 		267.4
(3) 		195.4
(-0.4) 		140.3
(-3) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 275
(3)
A 		185.2
(0.7)
A 		120.5
(-0.9)
A 		163.5
(0.2)
A 		150.8
(-0.1)
A 		38.1
(-3)
A 		237
B 262.7
(3)
A 		209.1
(1.3)
A 		128.1
(-1.2)
A 		184.3
(0.5)
A 		150
(-0.6)
A 		72.4
(-3)
A 		190
C 193.9
(1.9)
P 		215.8
(3)
A 		131.6
(-1.4)
A 		163
(0.2)
A 		148.1
(-0.5)
A 		100.9
(-3)
A 		115
avr 243.9
(3) 		203.4
(1.6) 		126.7
(-1.1) 		170.3
(0.5) 		149.6
(-0.3) 		70.5
(-3) 		173
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 87.2
(3)
M 		52.3
(-0.1)
A 		48.7
(-0.5)
A 		20.3
(-3)
A 		67
B 72
(2.7)
A 		74.3
(3)
A 		39.9
(-1.1)
A 		24.2
(-3)
A 		50
C 79.9
(3)
A 		74.9
(2.4)
A 		79.4
(2.9)
A 		28.4
(-3)
A 		52
avr 79.7
(3) 		67.2
(1.6) 		56
(0.4) 		24.3
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 50.7
(1.4)
A 		65.9
(3)
A 		26.2
(-1.2)
A 		41.5
(0.4)
A 		45.5
(0.8)
A 		9.3
(-3)
A 		57
B 91.4
(3)
A 		69.6
(1)
A 		31.1
(-2.6)
A 		48.8
(-1)
A 		60.9
(0.2)
A 		27
(-3)
A 		64
C 71.9
(3)
P 		65.8
(2.1)
A 		33.5
(-2.9)
A 		32.8
(-3)
A 		47.9
(-0.7)
A 		33.8
(-2.8)
A 		39
avr 71.3
(3) 		67.1
(2.5) 		30.3
(-2.1) 		41
(-0.8) 		51.4
(0.5) 		23.4
(-3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182801_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15201
Sequence Type Exemplar sequence
Representative Public ID C13D9.7 NCBI
Target Description C13D9.7 /REP_DB=WormBase Gene ID /WP=CE08140 /TR=O16241 /GB=AAB65308.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071940(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071940 NCBI Caenorhabditis elegans Na/Ca eXchangers family member (ncx-8) (ncx-8) mRNA, complete cds. 11/11 None
SNAP00000031103 ENSEMBL cdna:SNAP chromosome:CEL140:V:4983724:4986865:-1 10/11 None
C13D9.7.2 ENSEMBL cdna:known chromosome:CEL140:V:4983322:4986970:-1 gene:C13D9.7 11/11 None
C13D9.7.1 ENSEMBL cdna:known chromosome:CEL140:V:4983563:4987011:-1 gene:C13D9.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000031116 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4983723-4986970(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ncx-8
Ensembl C13D9.7
Entrez Gene 182575 Entrez gene
SwissProt O16241 EMBL-EBI
WormBase CE08140 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1633953_AT dm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65308 Na/ca exchangers protein 8 [Caenorhabditis elegans] ref|NP_504341.1| Na/Ca eXchangers family member (ncx-8) [Caenorhabditis elegans] 0.0
blast CAE64039 Hypothetical protein CBG08637 [Caenorhabditis briggsae] 0.0
blast AAB65309 Na/ca exchangers protein 9 [Caenorhabditis elegans] ref|NP_504342.1| Na/Ca eXchangers family member (ncx-9) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.7E-28
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 8.6E-26
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 1.7E-28
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 7.1E-15
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 2.9E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504341.1 11 4-26,101-123,175-197,204-226,236-258,482-501,511-533,546-568,611-633,646-668,678-700
C13D9.7.2 11 4-26,101-123,175-197,204-226,236-258,482-501,511-533,546-568,611-633,646-668,678-700
C13D9.7.1 11 4-26,101-123,175-197,204-226,236-258,482-501,511-533,546-568,611-633,646-668,678-700
SNAP00000031103 10 66-88,140-162,169-191,201-223,447-466,476-498,511-530,545-567,669-691,701-723
Sequence
Target Sequence 
>C. ELEGANS:182801_AT
tctacggactcgcattttccgcaaccattgctttgttggctctatgctttctcccactca
acaaagagttaaaataccacaaggaagtatactcgtaccttggttttcttatgtcaatag
catggatctacgcaaccgcaaatgaaattattagtgtaatgacaatggtcggtgtcgtga
ctggattgtcgcaagaattgttgggattgactgtgatgtcatggagtgattgcattgggg
atattgtcgcggatattgcggttatcaagcaaggatacccaaagatggctatggctgctg
caattggaggcccgcttttcaacttgctcattggattcggactcccattcactattgctt
ccgcgcaaggaaaacaaattgaccttattatcaatccagtataccgtattcttctcctct
ttcttggaatctctcttgtcacaacatttgtggcacttttcatcgaaaggttcaccgtga
gatggcccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTACGGACTCGCATTTTCCGCAAC 524 613 1553 Antisense
ATTGCTTTGTTGGCTCTATGCTTTC 277 7 1579 Antisense
AGCATGGATCTACGCAACCGCAAAT 519 77 1671 Antisense
GTCGGTGTCGTGACTGGATTGTCGC 259 473 1720 Antisense
ATGGCTGCTGCAATTGGAGGCCCGC 623 51 1843 Antisense
CCGCTTTTCAACTTGCTCATTGGAT 481 263 1864 Antisense
TCATTGGATTCGGACTCCCATTCAC 20 615 1880 Antisense
TTCACTATTGCTTCCGCGCAAGGAA 347 687 1900 Antisense
ATCCAGTATACCGTATTCTTCTCCT 589 39 1946 Antisense
CTTTCTTGGAATCTCTCTTGTCACA 401 223 1971 Antisense
AAAGGTTCACCGTGAGATGGCCCCA 337 121 2018 Antisense
Sequence Source Affymetrix Proprietary Database