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[182799_at]

Description W03F8.9 /REP_DB=WormBase Gene ID /WP=CE17278 /TR=O44571 /GB=AAB94191.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 22      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 73.9
(3)
M 		11.3
(-3)
A 		34.1
(-0.8)
A 		44.8
(0.2)
A 		63
B 86.9
(3)
P 		75.2
(1.8)
A 		26.7
(-3)
A 		81.1
(2.4)
A 		60
C 72.1
(0)
P 		32.7
(-3)
A 		37.7
(-2.6)
A 		111.2
(3)
P 		79
avr 77.6
(2.8) 		39.7
(-2.1) 		32.8
(-3) 		79
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 52.1
(2.1)
A 		44.9
(0.6)
A 		26.8
(-3)
A 		56.7
(3)
A 		36.9
(-1)
A 		36.5
(-1.1)
A 		30
B 110.4
(3)
P 		97.4
(2.2)
A 		8.8
(-3)
A 		28.7
(-1.8)
A 		45.3
(-0.8)
A 		44.1
(-0.9)
A 		102
C 189.4
(3)
P 		104.8
(0.1)
P 		61.1
(-1.4)
A 		20.2
(-2.8)
A 		15.1
(-3)
A 		45.9
(-1.9)
A 		174
avr 117.3
(3) 		82.4
(0.5) 		32.2
(-3) 		35.2
(-2.8) 		32.4
(-3) 		42.2
(-2.3) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 26.9
(3)
M 		2.5
(-3)
A 		8.9
(-1.4)
A 		6.6
(-2)
A 		24
B 27.7
(3)
P 		18.6
(0.5)
A 		6.2
(-3)
A 		17.3
(0.1)
A 		22
C 23.9
(3)
P 		9.4
(-3)
A 		13.1
(-1.5)
A 		18.8
(0.9)
P 		15
avr 26.2
(3) 		10.2
(-2.7) 		9.4
(-3) 		14.2
(-1.3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 9.6
(-0.8)
A 		16
(3)
A 		5.8
(-3)
A 		14.4
(2.1)
A 		11.1
(0.1)
A 		8.9
(-1.2)
A 		10
B 38.4
(3)
P 		32.4
(2)
A 		2.1
(-3)
A 		7.6
(-2.1)
A 		18.4
(-0.3)
A 		16.4
(-0.6)
A 		36
C 70.2
(3)
P 		32
(-0.5)
P 		15.6
(-2)
A 		4.1
(-3)
A 		4.9
(-2.9)
A 		15.4
(-2)
A 		66
avr 39.4
(3) 		26.8
(0.6) 		7.8
(-3) 		8.7
(-2.8) 		11.5
(-2.3) 		13.6
(-1.9) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182799_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10548
Sequence Type Exemplar sequence
Representative Public ID W03F8.9 NCBI
Target Description W03F8.9 /REP_DB=WormBase Gene ID /WP=CE17278 /TR=O44571 /GB=AAB94191.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068339(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068339 NCBI Caenorhabditis elegans W03F8.9 (W03F8.9) mRNA, complete cds. 11/11 None
GENEFINDER00000036716 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5193772:5195589:-1 11/11 None
W03F8.9 ENSEMBL cdna:known chromosome:CEL140:IV:5193748:5195406:-1 gene:W03F8.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036705 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5193753-5195412(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F8.9
Entrez Gene 189161 Entrez gene
SwissProt O44571 EMBL-EBI
WormBase CE17278 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94191 Hypothetical protein W03F8.9 [Caenorhabditis elegans] ref|NP_500740.1| W03F8.9 [Caenorhabditis elegans] 1.0E-122
blast AAB94191 Hypothetical protein W03F8.9 [Caenorhabditis elegans] ref|NP_500740.1| W03F8.9 [Caenorhabditis elegans] 1.0E-100
blast CAE72826 Hypothetical protein CBG20108 [Caenorhabditis briggsae] 2.0E-82
blast CAE72826 Hypothetical protein CBG20108 [Caenorhabditis briggsae] 2.0E-62
blast CAA99838 Hypothetical protein F18E2.5 [Caenorhabditis elegans] emb|CAA99780.2| Hypothetical protein F18E2.5 [Caenorhabditis elegans] ref|NP_506190.2| G Protein, Alpha subunit family member (gpa-13) [Caenorhabditis elegans] gb|AAG32088.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q9XTB2|GPA13_CAEEL Guanine nucleotide-binding protein alpha-13 subunit 4.0E-11
Sequence
Target Sequence 
>C. ELEGANS:182799_AT
tgcgttcaactcaatcttcttaatatttaccgtgaactgaaaaacgtcatagaagctttg
gaaattcctatcagtgaagatcaggtaacaattttgaacaattttgatagcaaatcaact
tttacaaaacaaaggttcacccaattagatgaataccgtcaccgagaggttttccctcca
aaaatcattgaagcaatgaaggagctgtttgaatcgggtctctacgagttatgcagactt
cgacaacgaattcttccacttccacaaaactatcattttctatttcaacgtggcgatgac
tttatgaatccggaatatgtaccctcagaactggaaataatgatgtcatactcacagact
tgtggcttaaatcgggaaaatgtcacgtgtcaaggatataagtttgagcttctcgaaatg
ccaggacatcatctgtggagagcaaaatgggctgattattttgatgatccaaatcttgtc
gtttttgtaattgatctcagtgaactttgtgatccggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGTTCAACTCAATCTTCTTAATA 583 583 94 Antisense
TGAATACCGTCACCGAGAGGTTTTC 29 573 243 Antisense
GAGGTTTTCCCTCCAAAAATCATTG 87 403 259 Antisense
GGAGCTGTTTGAATCGGGTCTCTAC 103 521 294 Antisense
CTTCGACAACGAATTCTTCCACTTC 259 219 331 Antisense
CTTCCACTTCCACAAAACTATCATT 106 219 346 Antisense
TATCATTTTCTATTTCAACGTGGCG 514 659 364 Antisense
GAATCCGGAATATGTACCCTCAGAA 8 331 399 Antisense
CACAGACTTGTGGCTTAAATCGGGA 275 195 446 Antisense
GAGCTTCTCGAAATGCCAGGACATC 180 389 499 Antisense
ATCTCAGTGAACTTTGTGATCCGGC 545 31 587 Antisense
Sequence Source Affymetrix Proprietary Database