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[182789_at]

Description F25A2.1 /REP_DB=WormBase Gene ID /WP=CE09618 /TR=O16189 /GB=AAB65254.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 31      4grp_total > 50      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 35.3
(-3)
A 		57.5
(0.6)
A 		71.8
(3)
A 		41.3
(-2)
A 		37
B 42.3
(0.1)
A 		71.1
(3)
A 		60.3
(1.9)
A 		10.6
(-3)
A 		61
C 66.7
(2.1)
A 		63.8
(1.4)
A 		44.7
(-3)
A 		70.7
(3)
A 		26
avr 48.1
(-1.1) 		64.1
(3) 		58.9
(1.7) 		40.9
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 63
(2.6)
A 		66.8
(3)
P 		52.4
(1.7)
A 		51.5
(1.6)
M 		41.8
(0.7)
A 		2.5
(-3)
A 		64
B 60.3
(2.7)
P 		62.5
(3)
P 		61.5
(2.9)
A 		50.6
(1.3)
A 		24.4
(-2.6)
A 		21.6
(-3)
A 		41
C 46.5
(-1.8)
A 		82.8
(1.2)
A 		64.4
(-0.3)
A 		105.7
(3)
A 		46.3
(-1.8)
M 		31.4
(-3)
A 		74
avr 56.6
(1.4) 		70.7
(3) 		59.4
(1.7) 		69.3
(2.8) 		37.5
(-0.8) 		18.5
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 12.8
(0.2)
A 		12.5
(0)
A 		18.8
(3)
A 		6
(-3)
A 		13
B 13.5
(1.4)
A 		17.6
(3)
A 		14
(1.6)
A 		2.3
(-3)
A 		15
C 22.1
(3)
A 		18.3
(0.8)
A 		15.6
(-0.8)
A 		11.9
(-3)
A 		10
avr 16.1
(3) 		16.1
(3) 		16.1
(3) 		6.7
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 11.6
(-0.1)
A 		23.7
(3)
P 		11.4
(-0.2)
A 		13.1
(0.2)
M 		12.6
(0.1)
A 		0.6
(-3)
A 		23
B 21
(3)
P 		20.8
(2.9)
P 		14.9
(0.2)
A 		13.4
(-0.5)
A 		9.9
(-2.2)
A 		8.1
(-3)
A 		13
C 17.3
(-0.2)
A 		25.3
(3)
A 		16.4
(-0.6)
A 		21.2
(1.3)
A 		15
(-1.2)
M 		10.5
(-3)
A 		15
avr 16.6
(0.6) 		23.3
(3) 		14.2
(-0.2) 		15.9
(0.4) 		12.5
(-0.8) 		6.4
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182789_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14899
Sequence Type Exemplar sequence
Representative Public ID F25A2.1 NCBI
Target Description F25A2.1 /REP_DB=WormBase Gene ID /WP=CE09618 /TR=O16189 /GB=AAB65254.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070989(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070989 NCBI Caenorhabditis elegans F25A2.1 (F25A2.1) mRNA, complete cds. 11/11 None
F25A2.1 ENSEMBL cdna:known chromosome:CEL140:V:1179459:1179958:1 gene:F25A2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1179458-1179958(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25A2.1
Entrez Gene 184913 Entrez gene
SwissProt O16189 EMBL-EBI
WormBase CE09618 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65254 Hypothetical protein F25A2.1 [Caenorhabditis elegans] ref|NP_503390.1| F25A2.1 [Caenorhabditis elegans] 4.0E-83
blast CAE58019 Hypothetical protein CBG01090 [Caenorhabditis briggsae] 7.0E-44
blast CAE58023 Hypothetical protein CBG01095 [Caenorhabditis briggsae] 8.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR002921 EMBL-EBI Lipase, class 3 2.3E-14
Sequence
Target Sequence 
>C. ELEGANS:182789_AT
aatttgcaaatcattctcccacagttcaataccatattccgatcagctctctcggaataa
actgatctatgcagccatctccgcgtacgcttctcctccacaaagttgcatgagcttggc
gtttcaaaattttcaagtcaaacctcagattactgtagcttgcgacacacttgatgatac
atgttctggatttactggagtcgatcacgaaagtcaggcgattttggtagcttttcgtgg
aactaatcgtaacgcacagctgctcgttgaagccgtagaaactgtgtttgcaaacaataa
atcctgggtttccggtgggcacgtctccgaatacttcagcgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTTGCAAATCATTCTCCCACAGT 601 173 36 Antisense
ATTCTCCCACAGTTCAATACCATAT 145 11 48 Antisense
CAATACCATATTCCGATCAGCTCTC 470 181 62 Antisense
AGCTCTCTCGGAATAAACTGATCTA 102 81 80 Antisense
CTTCTCCTCCACAAAGTTGCATGAG 266 221 125 Antisense
GTTGCATGAGCTTGGCGTTTCAAAA 296 439 140 Antisense
GTAGCTTGCGACACACTTGATGATA 581 457 190 Antisense
GATTTTGGTAGCTTTTCGTGGAACT 656 429 255 Antisense
CTAATCGTAACGCACAGCTGCTCGT 253 227 278 Antisense
CACAGCTGCTCGTTGAAGCCGTAGA 539 193 290 Antisense
CACGTCTCCGAATACTTCAGCGATG 643 199 355 Antisense
Sequence Source Affymetrix Proprietary Database