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[182784_at]

Description F53C3.8 /REP_DB=WormBase Gene ID /WP=CE19446 /TR=Q9TXT1 /GB=AAC67459.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 05      4grp_total > 28      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 54.5
(-3)
A 		109.3
(1.6)
A 		125.8
(3)
A 		82.8
(-0.6)
A 		71
B 82
(3)
A 		55
(0.6)
A 		14.2
(-3)
A 		29.2
(-1.7)
A 		68
C 65.8
(-0.6)
A 		66.5
(-0.5)
A 		22.6
(-3)
A 		129.8
(3)
A 		107
avr 67.4
(0) 		76.9
(2.2) 		54.2
(-3) 		80.6
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 165.7
(3)
A 		3.4
(-3)
A 		69.3
(-0.6)
A 		5
(-2.9)
A 		64.4
(-0.7)
A 		10.4
(-2.7)
A 		162
B 12.4
(-3)
A 		38.2
(-0.4)
A 		72.7
(3)
A 		37.3
(-0.5)
A 		61.3
(1.9)
A 		24.8
(-1.8)
A 		60
C 46.8
(-1.7)
A 		159.2
(3)
A 		15.1
(-3)
A 		53.7
(-1.4)
A 		56.7
(-1.3)
A 		65.6
(-0.9)
A 		144
avr 75
(3) 		66.9
(1.9) 		52.4
(-0.2) 		32
(-3) 		60.8
(1) 		33.6
(-2.8) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 19.9
(-0.8)
A 		23.7
(0.3)
A 		32.9
(3)
A 		12.1
(-3)
A 		21
B 26.1
(3)
A 		13.6
(-0.3)
A 		3.3
(-3)
A 		6.2
(-2.2)
A 		23
C 21.8
(3)
A 		19.1
(1.8)
A 		7.9
(-3)
A 		21.9
(3)
A 		14
avr 22.6
(3) 		18.8
(0.5) 		14.7
(-2.2) 		13.4
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 30.5
(3)
A 		1.2
(-3)
A 		15.1
(-0.2)
A 		1.3
(-3)
A 		19.4
(0.7)
A 		2.5
(-2.7)
A 		29
B 4.3
(-3)
A 		12.7
(-0.6)
A 		17.6
(0.9)
A 		9.9
(-1.4)
A 		24.9
(3)
A 		9.2
(-1.6)
A 		21
C 17.3
(-1.2)
A 		48.6
(3)
A 		3.8
(-3)
A 		10.8
(-2.1)
A 		18.3
(-1.1)
A 		22
(-0.6)
A 		45
avr 17.4
(1.5) 		20.8
(3) 		12.2
(-0.8) 		7.3
(-3) 		20.9
(3) 		11.2
(-1.3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182784_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4796
Sequence Type Exemplar sequence
Representative Public ID F53C3.8 NCBI
Target Description F53C3.8 /REP_DB=WormBase Gene ID /WP=CE19446 /TR=Q9TXT1 /GB=AAC67459.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062284(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062284 NCBI Caenorhabditis elegans F53C3.8 (F53C3.8) mRNA, complete cds. 11/11 None
GENEFINDER00000027233 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3889423:3896511:1 11/11 None
F53C3.8 ENSEMBL cdna:known chromosome:CEL140:II:3891342:3892161:1 gene:F53C3.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027219 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3891340-3892160(+) 94.04 100.0
Public Domain and Genome References
Gene Title
Ensembl F53C3.8
Entrez Gene 186162 Entrez gene
SwissProt Q9TXT1 EMBL-EBI
WormBase CE19446 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67458 Hypothetical protein F53C3.7 [Caenorhabditis elegans] ref|NP_494686.1| F53C3.7 [Caenorhabditis elegans] 1.0E-129
blast AAC67459 Hypothetical protein F53C3.8 [Caenorhabditis elegans] ref|NP_494685.1| F53C3.8 [Caenorhabditis elegans] 6.0E-55
blast AAC67459 Hypothetical protein F53C3.8 [Caenorhabditis elegans] ref|NP_494685.1| F53C3.8 [Caenorhabditis elegans] 1.0E-42
Sequence
Target Sequence 
>C. ELEGANS:182784_AT
ttcagtgtccataaaaccctcttcaagccaaccaaaatgcagcattcccgctccaattcc
ccatcgtcgccaccaagaagacagccaaacaatttgttcaaccaaccggctagagctgtt
ttcccttcctttccagtcatgggagaagccgttccaatgaccttttttccgatgagtgga
tcaaacacttccacactttgtgcagctcctgccagaccagaacttcgagaaaattcggtg
gagatcgtcgagaggaactctgctccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAGTGTCCATAAAACCCTCTTCA 556 687 52 Antisense
ACCCTCTTCAAGCCAACCAAAATGC 221 87 67 Antisense
AACCAAAATGCAGCATTCCCGCTCC 223 149 81 Antisense
CCATCGTCGCCACCAAGAAGACAGC 587 271 112 Antisense
ACAATTTGTTCAACCAACCGGCTAG 338 115 140 Antisense
CAACCAACCGGCTAGAGCTGTTTTC 154 187 150 Antisense
CCCTTCCTTTCCAGTCATGGGAGAA 395 257 174 Antisense
TTCCAATGACCTTTTTTCCGATGAG 45 699 203 Antisense
AAACACTTCCACACTTTGTGCAGCT 359 129 234 Antisense
CTCCTGCCAGACCAGAACTTCGAGA 293 235 257 Antisense
ATCGTCGAGAGGAACTCTGCTCCAC 274 33 295 Antisense
Sequence Source Affymetrix Proprietary Database