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[182781_s_at]

Description F16H9.1B /REP_DB=WormBase Gene ID /WP=CE23666 /TR=Q9TVK0 /GB=CAB54230.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 06      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 60      6grp_total > 06      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 964
1 2 3 4 range
A 1265.9
(3)
P 		479.9
(-3)
P 		480.1
(-3)
P 		594.1
(-2.1)
P 		786
B 918.7
(3)
P 		611.5
(-0.4)
P 		376.1
(-3)
P 		492.8
(-1.7)
P 		543
C 1281.1
(3)
P 		417.8
(-2.4)
P 		317.4
(-3)
P 		497.8
(-1.9)
P 		964
avr 1155.2
(3) 		503.1
(-2.1) 		391.2
(-3) 		528.2
(-1.9) 		764

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 597
1 2 3 4 5 6 range
A 783.7
(3)
P 		460.1
(-0.5)
P 		429.1
(-0.8)
P 		297.2
(-2.2)
P 		413.8
(-1)
P 		222.1
(-3)
P 		562
B 603.8
(3)
P 		401.2
(0.1)
P 		427
(0.4)
P 		188.4
(-3)
P 		322.6
(-1.1)
P 		204.5
(-2.8)
P 		415
C 785.3
(3)
P 		436.2
(-0.7)
P 		349.4
(-1.6)
P 		330.1
(-1.8)
P 		300.8
(-2.1)
P 		212.1
(-3)
P 		573
avr 724.3
(3) 		432.5
(-0.4) 		401.8
(-0.8) 		271.9
(-2.3) 		345.7
(-1.4) 		212.9
(-3) 		511
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 377
1 2 3 4 range
A 460.9
(3)
P 		104.2
(-2.7)
P 		125.5
(-2.4)
P 		87
(-3)
P 		374
B 293
(3)
P 		151
(-1.1)
P 		87.1
(-3)
P 		105
(-2.5)
P 		206
C 424.1
(3)
P 		120.1
(-2.4)
P 		110.6
(-2.5)
P 		83.9
(-3)
P 		340
avr 392.7
(3) 		125.1
(-2.3) 		107.7
(-2.7) 		92
(-3) 		301

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 144.5
(2)
P 		163.6
(3)
P 		93.5
(-0.8)
P 		75.4
(-1.8)
P 		124.9
(0.9)
P 		54.1
(-3)
P 		110
B 210.1
(3)
P 		133.5
(0.1)
P 		103.7
(-1)
P 		49.9
(-3)
P 		131.1
(0)
P 		76.2
(-2)
P 		160
C 291.1
(3)
P 		133.1
(-1.2)
P 		88.9
(-2.4)
P 		66.4
(-3)
P 		97.3
(-2.2)
P 		71
(-2.9)
P 		225
avr 215.2
(3) 		143.4
(0.2) 		95.4
(-1.8) 		63.9
(-3) 		117.8
(-0.9) 		67.1
(-2.9) 		151
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182781_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17994
Sequence Type Exemplar sequence
Representative Public ID F16H9.1B NCBI
Target Description F16H9.1B /REP_DB=WormBase Gene ID /WP=CE23666 /TR=Q9TVK0 /GB=CAB54230.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029415(11), NM_077723(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029415 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-2) (rgs-2) mRNA, complete cds. 11/11 None
NM_077723 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-2) (rgs-2) mRNA, complete cds. 11/11 A 
F16H9.1b ENSEMBL cdna:known chromosome:CEL140:X:12712571:12727532:1 gene:F16H9.1 11/11 A 
F16H9.1a ENSEMBL cdna:known chromosome:CEL140:X:12721766:12728308:1 gene:F16H9.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002359 7/11 Cross Hyb Matching Probes None
GENEFINDER00000002370 7/11 Cross Hyb Matching Probes None
GENEFINDER00000043623 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12712578-12727491(+) 100.0 100.0
Public Domain and Genome References
Ensembl F16H9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54219 Hypothetical protein F16H9.1b [Caenorhabditis elegans] emb|CAB54230.1| Hypothetical protein F16H9.1b [Caenorhabditis elegans] ref|NP_001024586.1| Regulator of G protein Signaling family member (rgs-2) [Caenorhabditis elegans] 1.0E-101
blast CAA90295 Hypothetical protein F16H9.1a [Caenorhabditis elegans] emb|CAA91803.1| Hypothetical protein F16H9.1a [Caenorhabditis elegans] ref|NP_510124.2| Regulator of G protein Signaling family member (rgs-2) [Caenorhabditis elegans] gb|AAF33782.1| RGS-2 [Caenorhabditis elegans] sp|P49808|RGS2_CAEEL Regulator of G-protein signaling rgs-2 7.0E-94
blast CAB54219 Hypothetical protein F16H9.1b [Caenorhabditis elegans] emb|CAB54230.1| Hypothetical protein F16H9.1b [Caenorhabditis elegans] ref|NP_001024586.1| Regulator of G protein Signaling family member (rgs-2) [Caenorhabditis elegans] 9.0E-84
blast CAA90295 Hypothetical protein F16H9.1a [Caenorhabditis elegans] emb|CAA91803.1| Hypothetical protein F16H9.1a [Caenorhabditis elegans] ref|NP_510124.2| Regulator of G protein Signaling family member (rgs-2) [Caenorhabditis elegans] gb|AAF33782.1| RGS-2 [Caenorhabditis elegans] sp|P49808|RGS2_CAEEL Regulator of G-protein signaling rgs-2 1.0E-83
blast CAE57222 Hypothetical protein CBG00085 [Caenorhabditis briggsae] 6.0E-83
Protein Domains
Database ID Description E-Value
Pfam IPR000342 EMBL-EBI Regulator of G protein 6.6E-57
Pfam IPR000342 EMBL-EBI Regulator of G protein 6.6E-57
Sequence
Target Sequence 
>C. ELEGANS:182781_S_AT
ggcccacccacttatgaaattgttttcggatggtcgcaatcttttgagaacctcatgaaa
caccgagctggacaaaagtactttgccgaattcttaaaaggagaatactcagatgaaaac
attcttttctggcaggcgtgtgaagagctcaaacgagagaaaaatgccgagaaaattgaa
gagaaggctcgcattatctacgaagatttcatctcgatcctttccccaaaagaagtttcg
ttagactctcgtgtccgtgaaatcgttaacaccaacatgggacgtccatctgcctccacc
tttgacgaggctcaaaaccagatctacacccttatgcaaagagacagctacccacgattc
ttggcctccaacatctacaagaccgtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCCACCCACTTATGAAATTGTTT 474 541 136 Antisense
GTTTTCGGATGGTCGCAATCTTTTG 564 447 157 Antisense
AACATTCTTTTCTGGCAGGCGTGTG 106 139 253 Antisense
GAGAAGGCTCGCATTATCTACGAAG 426 391 316 Antisense
TCTCGATCCTTTCCCCAAAAGAAGT 68 595 347 Antisense
TTCGTTAGACTCTCGTGTCCGTGAA 379 693 372 Antisense
ACACCAACATGGGACGTCCATCTGC 503 109 404 Antisense
CTGCCTCCACCTTTGACGAGGCTCA 585 235 425 Antisense
CAAAACCAGATCTACACCCTTATGC 708 189 448 Antisense
CAAAGAGACAGCTACCCACGATTCT 177 193 472 Antisense
GCCTCCAACATCTACAAGACCGTGA 640 281 499 Antisense
Sequence Source Affymetrix Proprietary Database