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[182777_at]

Description F53C3.5 /REP_DB=WormBase Gene ID /WP=CE19443 /TR=Q9TXT4 /GB=AAC67456.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 46      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 398
1 2 3 4 range
A 419.4
(3)
P 		165.7
(-1.2)
P 		98.9
(-2.3)
A 		54.2
(-3)
A 		365
B 430.8
(3)
P 		158
(-1.7)
A 		81.3
(-3)
A 		88.5
(-2.9)
A 		350
C 452.1
(3)
P 		149.4
(-1.9)
P 		82.7
(-3)
A 		89.5
(-2.9)
A 		369
avr 434.1
(3) 		157.7
(-1.6) 		87.6
(-2.8) 		77.4
(-3) 		357

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 727
1 2 3 4 5 6 range
A 486.3
(3)
P 		258.7
(-0.2)
P 		123.3
(-2.1)
M 		55.8
(-3)
A 		111.5
(-2.2)
A 		88.5
(-2.5)
A 		431
B 491.9
(3)
P 		250.2
(-0.4)
P 		139.1
(-2)
M 		92.5
(-2.7)
A 		70.8
(-3)
A 		69.7
(-3)
A 		422
C 748.6
(3)
P 		273.1
(-0.9)
P 		94.8
(-2.4)
A 		21.7
(-3)
A 		36.8
(-2.9)
A 		74.7
(-2.6)
A 		727
avr 575.6
(3) 		260.7
(-0.6) 		119.1
(-2.3) 		56.7
(-3) 		73
(-2.8) 		77.6
(-2.8) 		519
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 152.7
(3)
P 		36
(-1.8)
P 		25.8
(-2.3)
A 		7.9
(-3)
A 		145
B 137.4
(3)
P 		39
(-2)
A 		18.8
(-3)
A 		18.9
(-3)
A 		119
C 149.7
(3)
P 		42.9
(-1.8)
P 		28.8
(-2.4)
A 		15.1
(-3)
A 		135
avr 146.6
(3) 		39.3
(-1.9) 		24.5
(-2.5) 		14
(-3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 273
1 2 3 4 5 6 range
A 89.6
(2.8)
P 		92
(3)
P 		26.8
(-2)
M 		14.2
(-3)
A 		33.7
(-1.5)
A 		21.6
(-2.4)
A 		78
B 171.2
(3)
P 		83.3
(-0.6)
P 		33.8
(-2.6)
M 		24.5
(-3)
A 		28.8
(-2.8)
A 		26
(-2.9)
A 		147
C 277.5
(3)
P 		83.3
(-1.3)
P 		24.1
(-2.6)
A 		4.4
(-3)
A 		11.9
(-2.8)
A 		25
(-2.5)
A 		273
avr 179.4
(3) 		86.2
(-0.4) 		28.2
(-2.5) 		14.4
(-3) 		24.8
(-2.6) 		24.2
(-2.6) 		165
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182777_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5356
Sequence Type Exemplar sequence
Representative Public ID F53C3.5 NCBI
Target Description F53C3.5 /REP_DB=WormBase Gene ID /WP=CE19443 /TR=Q9TXT4 /GB=AAC67456.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062288(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062288 NCBI Caenorhabditis elegans F53C3.5 (F53C3.5) mRNA, complete cds. 10/11 None
SNAP00000027222 ENSEMBL cdna:SNAP chromosome:CEL140:II:3900010:3900776:1 9/11 None
GENEFINDER00000027238 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3898964:3900776:1 9/11 None
F53C3.5 ENSEMBL cdna:known chromosome:CEL140:II:3899856:3900776:1 gene:F53C3.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3899831-3900775(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53C3.5
Entrez Gene 186160 Entrez gene
SwissProt Q9TXT4 EMBL-EBI
WormBase CE35940 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67456 Hypothetical protein F53C3.5 [Caenorhabditis elegans] ref|NP_494689.2| F53C3.5 [Caenorhabditis elegans] 1.0E-105
blast AAK73870 Hypothetical protein F53C3.6b [Caenorhabditis elegans] ref|NP_494688.1| F53C3.6b [Caenorhabditis elegans] 2.0E-59
blast AAK73869 Hypothetical protein F53C3.6a [Caenorhabditis elegans] ref|NP_494687.2| F53C3.6a [Caenorhabditis elegans] 2.0E-59
blast AAC67454 Hypothetical protein F53C3.3 [Caenorhabditis elegans] ref|NP_494691.2| F53C3.3 [Caenorhabditis elegans] 2.0E-55
blast AAC67455 Hypothetical protein F53C3.4 [Caenorhabditis elegans] ref|NP_494690.2| F53C3.4 [Caenorhabditis elegans] 1.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 2.4E-28
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 6.7E-28
Pfam IPR002619 EMBL-EBI Protein of unknown function CX 2.4E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494689.2 2 20-42,109-128
F53C3.5 2 20-42,109-128
SNAP00000027222 2 20-42,109-128
GENEFINDER00000027238 1 250-269
Sequence
Target Sequence 
>C. ELEGANS:182777_AT
agcaggaggctgccaacgtactggccccgtcttcaaatcaggagggatggactataggtc
gggtttccaacagaatgtattccattccaactcgacttccgaagcatttgcatattctcc
gatgactcatacttatgtcatcatctcacctgctactccgatgatttaccgaaactacca
ctactattgggcagggcatcatgtgaacactacagaaaaaccattcaagtgtaattatgt
aataactgatgaggacatggaactgagcaatgtggcattcgcaaatggcacaagaccggc
acttatcacctttggatgtgcagagtatacgtattgttgtggaatgacctgttgtcctag
tttcagtgatacacaactattaatattcattgtgttgttagtggcgggaattatttttgt
gctgatctgcactgagaaggcaaacgaaagacgtggtcaaggcagtccgaccacaagcta
caatcatccaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAGGAGGCTGCCAACGTACTGGC 211 73 72 Antisense
TGGCCCCGTCTTCAAATCAGGAGGG 99 545 93 Antisense
GACTATAGGTCGGGTTTCCAACAGA 222 363 121 Antisense
ATTCCAACTCGACTTCCGAAGCATT 691 5 155 Antisense
TGCATATTCTCCGATGACTCATACT 71 579 180 Antisense
CATACTTATGTCATCATCTCACCTG 6 215 199 Antisense
ACCTGCTACTCCGATGATTTACCGA 62 89 219 Antisense
GATTTACCGAAACTACCACTACTAT 61 429 234 Antisense
AAATGGCACAAGACCGGCACTTATC 420 121 354 Antisense
GAATGACCTGTTGTCCTAGTTTCAG 458 327 413 Antisense
CGACCACAAGCTACAATCATCCAAG 366 251 539 Antisense
Sequence Source Affymetrix Proprietary Database