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[182776_at]

Description Y45F3A.8 /REP_DB=WormBase Gene ID /WP=CE19169 /TR=Q9XWZ0 /GB=CAA21492.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 49      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 3.1
(-3)
A 		128.7
(3)
A 		111.7
(2.2)
M 		72.4
(0.3)
A 		126
B 2.5
(-3)
A 		193.1
(3)
P 		150.8
(1.7)
A 		2.8
(-3)
A 		191
C 2.3
(-3)
A 		157.1
(3)
A 		122.3
(1.7)
A 		3.2
(-3)
A 		155
avr 2.6
(-3) 		159.6
(3) 		128.3
(1.8) 		26.1
(-2.1) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 4.2
(-3)
A 		90.4
(3)
P 		10.1
(-2.6)
A 		62.5
(1.1)
A 		77.6
(2.1)
A 		33.2
(-1)
A 		86
B 2.3
(-3)
A 		98.2
(3)
A 		66.9
(1)
A 		53.3
(0.2)
A 		63.6
(0.8)
A 		56.4
(0.4)
A 		96
C 2.5
(-3)
A 		38.6
(0.1)
A 		26.4
(-0.9)
A 		37.5
(0)
A 		71.6
(3)
A 		35.1
(-0.2)
A 		69
avr 3
(-3) 		75.7
(3) 		34.5
(-0.4) 		51.1
(1) 		70.9
(2.6) 		41.6
(0.2) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 1.1
(-3)
A 		27.9
(2.7)
A 		29.2
(3)
M 		10.6
(-1)
A 		28
B 0.8
(-3)
A 		47.7
(3)
P 		34.9
(1.4)
A 		0.6
(-3)
A 		47
C 0.8
(-3)
A 		45.2
(3)
A 		42.6
(2.7)
A 		0.5
(-3)
A 		45
avr 0.9
(-3) 		40.3
(3) 		35.6
(2.3) 		3.9
(-2.5) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 0.8
(-3)
A 		32.2
(3)
P 		2.2
(-2.7)
A 		15.9
(-0.1)
A 		23.4
(1.3)
A 		8.1
(-1.6)
A 		31
B 0.8
(-3)
A 		32.7
(3)
A 		16.2
(-0.1)
A 		14.1
(-0.5)
A 		25.8
(1.7)
A 		21
(0.8)
A 		32
C 0.9
(-3)
A 		11.8
(-0.1)
A 		6.7
(-1.4)
A 		7.5
(-1.2)
A 		23.2
(3)
A 		11.8
(-0.1)
A 		22
avr 0.8
(-3) 		25.6
(3) 		8.4
(-1.2) 		12.5
(-0.2) 		24.1
(2.6) 		13.6
(0.1) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182776_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6202
Sequence Type Exemplar sequence
Representative Public ID Y45F3A.8 NCBI
Target Description Y45F3A.8 /REP_DB=WormBase Gene ID /WP=CE19169 /TR=Q9XWZ0 /GB=CAA21492.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066931(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066931 NCBI Caenorhabditis elegans Y45F3A.8 (Y45F3A.8) mRNA, complete cds. 10/11 None
SNAP00000002982 ENSEMBL cdna:SNAP chromosome:CEL140:III:10589820:10591798:-1 10/11 None
GENEFINDER00000002992 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10589820:10592100:-1 11/11 None
Y45F3A.8 ENSEMBL cdna:known chromosome:CEL140:III:10589759:10592666:-1 gene:Y45F3A.8 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10589815-10592096(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y45F3A.8
Entrez Gene 189910 Entrez gene
SwissProt Q9XWZ0 EMBL-EBI
WormBase CE37285 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21492 Hypothetical protein Y45F3A.8 [Caenorhabditis elegans] ref|NP_499332.2| Y45F3A.8 [Caenorhabditis elegans] 0.0
blast CAA21492 Hypothetical protein Y45F3A.8 [Caenorhabditis elegans] ref|NP_499332.2| Y45F3A.8 [Caenorhabditis elegans] 1.0E-161
blast CAA21492 Hypothetical protein Y45F3A.8 [Caenorhabditis elegans] ref|NP_499332.2| Y45F3A.8 [Caenorhabditis elegans] 1.0E-107
blast CAE71443 Hypothetical protein CBG18354 [Caenorhabditis briggsae] 1.0E-93
blast CAE71443 Hypothetical protein CBG18354 [Caenorhabditis briggsae] 2.0E-93
blast CAE71443 Hypothetical protein CBG18354 [Caenorhabditis briggsae] 3.0E-93
blast CAI46589 Hypothetical protein E04D5.4 [Caenorhabditis elegans] emb|CAA91281.3| Hypothetical protein E04D5.4 [Caenorhabditis elegans] ref|NP_496243.2| E04D5.4 [Caenorhabditis elegans] 1.0E-15
blast CAI46589 Hypothetical protein E04D5.4 [Caenorhabditis elegans] emb|CAA91281.3| Hypothetical protein E04D5.4 [Caenorhabditis elegans] ref|NP_496243.2| E04D5.4 [Caenorhabditis elegans] 3.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.033
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.1E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.5E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-6
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.1E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.5E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-6
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.1E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 9.5E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-6
Sequence
Target Sequence 
>C. ELEGANS:182776_AT
accagctgttgtgactctcagcggccgacacctcattggaccggatgggccacgctctag
atctccccgaagctctgaatcccggcataatgagaccacatcgattactttaccatcttc
cttcaattctacagtcaggaagctgacactaaaagaattgatgactacgagggacacaat
gccaaagatcactacagccgaaccgagctccctgacaacgtcactttcaaagacagtaat
tcaagggacatgcttcgatgagtacacttattgtagagaattcacatcactttgctctca
tccagcattctccgaagttatggcttcccattgctcattgacttgtgggagatgtgatga
agtggaaagagttgaagagggaaccgaagattgtgaggatatgacaccagactgcagaaa
ctaccgtgacctttgtgagcactcgagatacaagactcttatggagaactactgcccaaa
ggcctgtggtcattgtattccaatgtgtagagatcgtcatcagaattgtcccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCAGCTGTTGTGACTCTCAGCGGC 533 85 60 Antisense
CGAAGCTCTGAATCCCGGCATAATG 365 253 127 Antisense
GAGACCACATCGATTACTTTACCAT 102 393 151 Antisense
CATCTTCCTTCAATTCTACAGTCAG 448 213 173 Antisense
GCTCCCTGACAACGTCACTTTCAAA 487 299 266 Antisense
AGAATTCACATCACTTTGCTCTCAT 705 75 336 Antisense
CTCATCCAGCATTCTCCGAAGTTAT 548 229 356 Antisense
TTATGGCTTCCCATTGCTCATTGAC 222 681 377 Antisense
ACTACCGTGACCTTTGTGAGCACTC 438 101 479 Antisense
TACTGCCCAAAGGCCTGTGGTCATT 265 647 529 Antisense
GAGATCGTCATCAGAATTGTCCCCA 475 395 569 Antisense
Sequence Source Affymetrix Proprietary Database