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[182768_at]

Description F58D12.3 /REP_DB=WormBase Gene ID /WP=CE11370 /TR=O02277 /GB=CAB03145.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 60      4grp_total > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 7.9
(-2.4)
A 		12
(-2)
A 		0.7
(-3)
A 		70
(3)
P 		69
B 30.7
(1.8)
A 		2.6
(-3)
A 		10.6
(-1.6)
A 		37.9
(3)
A 		35
C 6.2
(-3)
A 		9.1
(-2.5)
A 		7.7
(-2.8)
A 		43.2
(3)
P 		37
avr 14.9
(-1.8) 		7.9
(-2.8) 		6.3
(-3) 		50.4
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 16
(-0.3)
A 		18.4
(0.2)
A 		32.4
(3)
A 		27.5
(2)
A 		3
(-3)
A 		2.8
(-3)
A 		30
B 20.6
(1.8)
A 		0.6
(-3)
A 		2.6
(-2.5)
A 		25.4
(3)
A 		7
(-1.5)
A 		5.3
(-1.9)
A 		25
C 1.2
(-3)
A 		10.5
(1.7)
A 		10
(1.4)
A 		4.6
(-1.3)
A 		10.3
(1.6)
A 		13.2
(3)
A 		12
avr 12.6
(-0.2) 		9.8
(-1.5) 		15
(1) 		19.2
(3) 		6.8
(-3) 		7.1
(-2.9) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2.9
(-1.4)
A 		2.6
(-1.6)
A 		0.2
(-3)
A 		10.2
(3)
P 		10
B 9.8
(3)
A 		0.6
(-3)
A 		2.5
(-1.8)
A 		8.1
(1.9)
A 		9
C 2
(-3)
A 		2.6
(-2.3)
A 		2.7
(-2.2)
A 		7.3
(3)
P 		5
avr 4.9
(-0.2) 		1.9
(-2.9) 		1.8
(-3) 		8.5
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 3
(-0.8)
A 		6.5
(2.5)
A 		7
(3)
A 		7
(3)
A 		0.9
(-2.8)
A 		0.7
(-3)
A 		6
B 7.2
(3)
A 		0.2
(-3)
A 		0.6
(-2.7)
A 		6.7
(2.6)
A 		2.8
(-0.8)
A 		2
(-1.5)
A 		7
C 0.5
(-3)
A 		3.2
(1.2)
A 		2.6
(0.2)
A 		0.9
(-2.4)
A 		3.3
(1.3)
A 		4.4
(3)
A 		4
avr 3.6
(-0) 		3.3
(-0.7) 		3.4
(-0.5) 		4.9
(3) 		2.3
(-3) 		2.4
(-2.8) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182768_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14166
Sequence Type Exemplar sequence
Representative Public ID F58D12.3 NCBI
Target Description F58D12.3 /REP_DB=WormBase Gene ID /WP=CE11370 /TR=O02277 /GB=CAB03145.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074090(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074090 NCBI Caenorhabditis elegans F58D12.3 (F58D12.3) mRNA, complete cds. 11/11 None
SNAP00000016874 ENSEMBL cdna:SNAP chromosome:CEL140:V:14061423:14066044:1 11/11 None
GENEFINDER00000016877 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14060463:14066044:1 11/11 None
F58D12.3 ENSEMBL cdna:known chromosome:CEL140:V:14060428:14066059:1 gene:F58D12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14060462-14066059(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58D12.3
Entrez Gene 186505 Entrez gene
SwissProt O02277 EMBL-EBI
WormBase CE37977 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03145 Hypothetical protein F58D12.3 [Caenorhabditis elegans] ref|NP_506491.2| F58D12.3 [Caenorhabditis elegans] 0.0
blast CAE61023 Hypothetical protein CBG04766 [Caenorhabditis briggsae] 1.0E-104
blast CAE61023 Hypothetical protein CBG04766 [Caenorhabditis briggsae] 1.0E-100
blast CAE61023 Hypothetical protein CBG04766 [Caenorhabditis briggsae] 3.0E-88
Sequence
Target Sequence 
>C. ELEGANS:182768_AT
gaactaatcaaagatgtgtccgctcttatggagaaattgaacacagttccattgaaatat
cgagcaaatggattggaagttgaatttgcgaaatacatcaatgatatattttgtgtttcc
ttcgatccaccaggagaaaccgatgaagttactataaaatctgaaaactgcctgactcct
caaacaacaggcgaaaaaatgttcaagcttggacattgtgaaagagcattgaatgagagg
gttaaagagggcgatatcgatggaaatgagcccagattcaagcttattcgagtcgaagct
gttcaaattctgacggatcgaggtgtatttgttagtggagttgaatccaaatttcagacg
ttttccgatgaagttaccgaatttcgaattaaatgttttgatgaagctttgctaaaccat
attgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTAATCAAAGATGTGTCCGCTC 244 349 1750 Antisense
GATGTGTCCGCTCTTATGGAGAAAT 145 409 1762 Antisense
GAACACAGTTCCATTGAAATATCGA 502 351 1788 Antisense
TGTTTCCTTCGATCCACCAGGAGAA 477 559 1863 Antisense
ACTGCCTGACTCCTCAAACAACAGG 555 97 1916 Antisense
GCCCAGATTCAAGCTTATTCGAGTC 396 287 2019 Antisense
GAAGCTGTTCAAATTCTGACGGATC 596 341 2044 Antisense
CTGACGGATCGAGGTGTATTTGTTA 707 237 2059 Antisense
GAATCCAAATTTCAGACGTTTTCCG 370 323 2092 Antisense
CAGACGTTTTCCGATGAAGTTACCG 580 201 2104 Antisense
GAAGCTTTGCTAAACCATATTGATG 224 343 2152 Antisense
Sequence Source Affymetrix Proprietary Database