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[182758_at]

Description B0261.4 /REP_DB=WormBase Gene ID /WP=CE07706 /TR=O01437 /GB=AAB52351.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 22      4grp_high_8_8 > 15      6grp_highest_nonorm > 77      6grp_total > 45      4grp_total > 01      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1027
1 2 3 4 range
A 1223.3
(-2.8)
P 		1195.4
(-3)
P 		1211
(-2.9)
P 		2222.6
(3)
P 		1027
B 1255.5
(-3)
P 		1267.7
(-2.9)
P 		1493.7
(-1.2)
P 		2042.1
(3)
P 		787
C 1313.8
(-3)
P 		1413.5
(-2)
P 		1522.2
(-1)
P 		1925
(3)
P 		611
avr 1264.2
(-3) 		1292.2
(-2.8) 		1409
(-1.9) 		2063.2
(3) 		799

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 680
1 2 3 4 5 6 range
A 1527.9
(3)
P 		1250.1
(-0.8)
P 		1346.6
(0.5)
P 		1091.3
(-3)
P 		1220.3
(-1.2)
P 		1108.3
(-2.8)
P 		437
B 1472
(0.7)
P 		1418.3
(-0.1)
P 		1648.8
(3)
P 		1238.4
(-2.4)
P 		1196.5
(-3)
P 		1391.4
(-0.4)
P 		452
C 1112.6
(-1.6)
P 		1570.3
(3)
P 		1163.3
(-1.1)
P 		1132.4
(-1.4)
P 		969.1
(-3)
P 		1539.2
(2.7)
P 		601
avr 1370.8
(2.1) 		1412.9
(3) 		1386.2
(2.4) 		1154
(-2.5) 		1128.6
(-3) 		1346.3
(1.6) 		284
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 271
1 2 3 4 range
A 445.4
(3)
P 		259.5
(-3)
P 		316.6
(-1.2)
P 		325.3
(-0.9)
P 		186
B 400.4
(1.3)
P 		313.1
(-3)
P 		346.1
(-1.4)
P 		435.2
(3)
P 		122
C 434.9
(0.2)
P 		406.4
(-0.6)
P 		530.5
(3)
P 		324.6
(-3)
P 		206
avr 426.9
(3) 		326.3
(-3) 		397.7
(1.3) 		361.7
(-0.9) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 281.7
(-2.6)
P 		444.6
(3)
P 		293.3
(-2.2)
P 		276.8
(-2.8)
P 		368.2
(0.4)
P 		269.8
(-3)
P 		175
B 512.2
(2.8)
P 		472.1
(1.5)
P 		400.4
(-0.7)
P 		328
(-3)
P 		486.1
(2)
P 		518.8
(3)
P 		191
C 412.4
(0.9)
P 		479.1
(2.2)
P 		296.1
(-1.6)
P 		227.7
(-3)
P 		313.6
(-1.2)
P 		515.3
(3)
P 		288
avr 402.1
(1) 		465.3
(3) 		329.9
(-1.3) 		277.5
(-3) 		389.3
(0.6) 		434.6
(2) 		188
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182758_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.619
Sequence Type Exemplar sequence
Representative Public ID B0261.4 NCBI
Target Description B0261.4 /REP_DB=WormBase Gene ID /WP=CE07706 /TR=O01437 /GB=AAB52351.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059147(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059147 NCBI Caenorhabditis elegans B0261.4 (B0261.4) mRNA, complete cds. 11/11 None
SNAP00000040715 ENSEMBL cdna:SNAP chromosome:CEL140:I:5243502:5262993:1 10/11 None
GENEFINDER00000040720 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5244265:5245295:1 11/11 None
B0261.4 ENSEMBL cdna:known chromosome:CEL140:I:5244265:5245295:1 gene:B0261.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5244264-5245295(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0261.4
Entrez Gene 172164 Entrez gene
SwissProt O01437 EMBL-EBI
WormBase CE07706 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast Q95Q95 Target of rapamycin homolog (CeTOR) (Lethal protein 363) pir||T25444 hypothetical protein B0261.2 - Caenorhabditis elegans 0.0
blast AAN84886 Lethal protein 363, isoform b [Caenorhabditis elegans] ref|NP_491552.2| LEThal family member (let-363) [Caenorhabditis elegans] 0.0
blast AAN84885 Lethal protein 363, isoform a [Caenorhabditis elegans] ref|NP_491549.2| LEThal family member (let-363) [Caenorhabditis elegans] 0.0
blast AAB52351 Hypothetical protein B0261.4 [Caenorhabditis elegans] ref|NP_491548.1| B0261.4 [Caenorhabditis elegans] 1.0E-144
blast CAE67373 Hypothetical protein CBG12849 [Caenorhabditis briggsae] 1.0E-111
blast EAA03797 ENSANGP00000013554 [Anopheles gambiae str. PEST] ref|XP_307966.2| ENSANGP00000013554 [Anopheles gambiae str. PEST] 2.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 1.1E-104
Pfam IPR010729 EMBL-EBI Mitochondrial 39-S ribosomal L47 9.6E-64
Pfam IPR003152 EMBL-EBI PIK-related kinase, FATC 3.8E-21
Pfam IPR003151 EMBL-EBI PIK-related kinase, FAT 1.0E-126
Pfam IPR010729 EMBL-EBI Mitochondrial 39-S ribosomal L47 9.6E-64
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491548.1 1 10-32
B0261.4 1 10-32
GENEFINDER00000040720 1 10-32
Sequence
Target Sequence 
>C. ELEGANS:182758_AT
tgtcttcggctgttcgatcacaaaatgccgtcaacacacgattttccagtacaatgaaac
aatttttcgatgatgaagcgaattttggaaaagcagagctccgcccgaaacatcggccgg
gcagatcgtggacagcagaagaactgagattaaaaagcaattcagatttgcataagcttt
ggtatgtgtgcttgaaggagcgaaatatgctgataacgatgaaaaaagcgcacacttcac
gtgcccggaacatgccaaacccagagagacttgatcgtgttcatgagacaatggatcgta
tagaatcagtagttcacgagcgaaatgatgcggtcttccgtttggaaactggagaatccg
cggctccacgcaagagaacaatcacttcattcgctggatttacatatcaaaaacaagcca
cagaacactttgcaccacctcaactcggccagaaagaatacgaaactccatatctcgacg
acgatgcctacgtgatgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCTTCGGCTGTTCGATCACAAAA 258 559 146 Antisense
AAATGCCGTCAACACACGATTTTCC 567 117 168 Antisense
TTTGGAAAAGCAGAGCTCCGCCCGA 101 665 229 Antisense
AACATCGGCCGGGCAGATCGTGGAC 532 135 254 Antisense
AAAAAAGCGCACACTTCACGTGCCC 197 129 367 Antisense
GAGCGAAATGATGCGGTCTTCCGTT 525 385 463 Antisense
TCCGCGGCTCCACGCAAGAGAACAA 97 597 502 Antisense
GAGAACAATCACTTCATTCGCTGGA 208 393 519 Antisense
ACAAGCCACAGAACACTTTGCACCA 272 113 558 Antisense
ACGAAACTCCATATCTCGACGACGA 207 93 605 Antisense
TCGACGACGATGCCTACGTGATGCA 71 601 620 Antisense
Sequence Source Affymetrix Proprietary Database