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[182757_at]

Description F53F4.14 /REP_DB=WormBase Gene ID /WP=CE23767 /TR=Q9TW43 /GB=CAB54259.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 57      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 82      6grp_total > 58      4grp_total > 05      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 943
1 2 3 4 range
A 359.6
(-0.8)
P 		161.9
(-2.8)
P 		138.6
(-3)
P 		740.5
(3)
P 		602
B 448.6
(-0.5)
P 		130
(-2.8)
P 		103.5
(-3)
P 		948.6
(3)
P 		845
C 428.5
(-1.2)
P 		180.4
(-2.8)
P 		156.9
(-3)
P 		1046.1
(3)
P 		889
avr 412.2
(-0.8) 		157.4
(-2.8) 		133
(-3) 		911.7
(3) 		779

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 506
1 2 3 4 5 6 range
A 438.7
(3)
P 		299.5
(-1.9)
P 		289.1
(-2.3)
P 		268.7
(-3)
P 		321.3
(-1.1)
P 		402.1
(1.7)
P 		170
B 403.9
(0.1)
P 		293.5
(-3)
P 		451.1
(1.4)
P 		357.3
(-1.2)
P 		372.5
(-0.8)
P 		507.1
(3)
P 		214
C 385.8
(-1)
P 		436.6
(-0.4)
P 		221
(-3)
P 		331.9
(-1.7)
P 		244.6
(-2.7)
P 		726.8
(3)
P 		506
avr 409.5
(-0.5) 		343.2
(-2.2) 		320.4
(-2.8) 		319.3
(-2.8) 		312.8
(-3) 		545.3
(3) 		233
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 130.9
(3)
P 		35.2
(-3)
P 		36.2
(-2.9)
P 		108.4
(1.6)
P 		96
B 143.1
(1)
P 		32.1
(-2.7)
P 		24
(-3)
P 		202.2
(3)
P 		178
C 141.8
(1.3)
P 		51.9
(-3)
P 		54.7
(-2.9)
P 		176.4
(3)
P 		125
avr 138.6
(1.9) 		39.7
(-2.9) 		38.3
(-3) 		162.3
(3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 187
1 2 3 4 5 6 range
A 80.9
(-0.5)
P 		106.5
(3)
P 		63
(-3)
P 		68.1
(-2.3)
P 		97
(1.7)
P 		97.9
(1.8)
P 		44
B 140.5
(-0.1)
P 		97.7
(-2.8)
P 		109.5
(-2.1)
P 		94.6
(-3)
P 		151.4
(0.6)
P 		189.1
(3)
P 		95
C 143
(-0.2)
P 		133.2
(-0.5)
P 		56.2
(-3)
P 		66.7
(-2.7)
P 		79.2
(-2.3)
P 		243.3
(3)
P 		187
avr 121.5
(-0.3) 		112.5
(-0.8) 		76.2
(-3) 		76.5
(-3) 		109.2
(-1) 		176.8
(3) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182757_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12701
Sequence Type Exemplar sequence
Representative Public ID F53F4.14 NCBI
Target Description F53F4.14 /REP_DB=WormBase Gene ID /WP=CE23767 /TR=Q9TW43 /GB=CAB54259.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073977(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073977 NCBI Caenorhabditis elegans F53F4.14 (F53F4.14) mRNA, complete cds. 11/11 None
SNAP00000004883 ENSEMBL cdna:SNAP chromosome:CEL140:V:13631322:13632289:1 10/11 None
F53F4.14.2 ENSEMBL cdna:known chromosome:CEL140:V:13630165:13632418:1 gene:F53F4.14 11/11 None
F53F4.14.1 ENSEMBL cdna:known chromosome:CEL140:V:13630205:13632418:1 gene:F53F4.14 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13629914-13632289(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F4.14
Entrez Gene 179852 Entrez gene
SwissProt Q9TW43 EMBL-EBI
WormBase CE29593 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16956_AT ubiquitin family protein at
CANINE:1596820_AT similar to CG5986-PA cfa
CANINE_2:CFA.19517.1.S1_S_AT similar to CG5986-PA cfa
CANINE_2:CFA.2713.2.A1_A_AT similar to CG5986-PA cfa
CANINE_2:CFA.2713.2.A1_S_AT similar to CG5986-PA cfa
DROSOPHILA_2:1624613_AT dm
DROSGENOME1:153487_AT dm
CHICKEN:GGA.9565.1.S1_AT similar to cDNA sequence BC031781 gga
CHICKEN:GGA.9565.2.S1_A_AT similar to cDNA sequence BC031781 gga
CHICKEN:GGA.9565.2.S1_S_AT similar to cDNA sequence BC031781 gga
HU35KSUBD:RC_AA449121_AT chromosome 1 open reading frame 55 hs
U133_X3P:HS2.99210.1.S1_3P_AT chromosome 1 open reading frame 55 hs
U133_X3P:HS.99210.0.A1_3P_AT chromosome 1 open reading frame 55 hs
HG-U133_PLUS_2:1553338_AT chromosome 1 open reading frame 55 hs
HG-U133_PLUS_2:244103_AT chromosome 1 open reading frame 55 hs
HG-U133B:244103_AT chromosome 1 open reading frame 55 hs
HG-U95B:57447_AT chromosome 1 open reading frame 55 hs
HG-U95C:60073_F_AT chromosome 1 open reading frame 55 hs
MG-U74BV2:163841_F_AT cDNA sequence BC031781 mm
MOE430B:1436511_AT cDNA sequence BC031781 mm
MOUSE430_2:1436511_AT cDNA sequence BC031781 mm
MOE430B:1436151_X_AT cDNA sequence BC031781 mm
MOUSE430_2:1436151_X_AT cDNA sequence BC031781 mm
MOE430B:1447554_AT cDNA sequence BC031781 mm
MOUSE430_2:1447554_AT cDNA sequence BC031781 mm
MG-U74BV2:112651_AT CDNA sequence BC031781 (BC031781), mRNA mm
MOE430B:1447804_X_AT CDNA sequence BC031781 (BC031781), mRNA mm
MOUSE430_2:1447804_X_AT CDNA sequence BC031781 (BC031781), mRNA mm
MOE430B:1459829_X_AT CDNA sequence BC031781 (BC031781), mRNA mm
MOUSE430_2:1459829_X_AT CDNA sequence BC031781 (BC031781), mRNA mm
MOE430B:1459853_X_AT CDNA sequence BC031781 (BC031781), mRNA mm
MOUSE430_2:1459853_X_AT CDNA sequence BC031781 (BC031781), mRNA mm
MG-U74CV2:167626_R_AT CDNA sequence BC031781 (BC031781), mRNA mm
MU19KSUBA:TC20293_AT CDNA sequence BC031781 (BC031781), mRNA mm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54256 Hypothetical protein F53F4.14 [Caenorhabditis elegans] emb|CAB54259.5| Hypothetical protein F53F4.14 [Caenorhabditis elegans] ref|NP_506378.1| F53F4.14 [Caenorhabditis elegans] 0.0
blast CAE66272 Hypothetical protein CBG11516 [Caenorhabditis briggsae] 1.0E-163
blast CAB54256 Hypothetical protein F53F4.14 [Caenorhabditis elegans] emb|CAB54259.5| Hypothetical protein F53F4.14 [Caenorhabditis elegans] ref|NP_506378.1| F53F4.14 [Caenorhabditis elegans] 6.0E-75
blast CAE66272 Hypothetical protein CBG11516 [Caenorhabditis briggsae] 6.0E-48
blast XP_785872 PREDICTED: similar to CG5986-PA [Strongylocentrotus purpuratus] 7.0E-23
Sequence
Target Sequence 
>C. ELEGANS:182757_AT
gaagacgtgactgatctgttcaatgatagaggtggaagaaagcgaaaaattgcagctcca
agtattggtgaagacggaggaaataaacgaatcaatgatgaaagcgatgatagtgaagat
gattcagaaaatgaagtggatccagaagaacttgaagctattcgtcaatatttcgaagct
aaaaagcaagatgagaaggataatggtgaaggaacatccgaagcagttccagaagtttta
gttgacgagcaagaaatagaaccagcaaagcgtccaagacttgatagcgcttctaacatt
gacgatcttccaaaaatcgacgttaaaactccttgtgaatatggtccaatctcgctcgac
gatttcaccagtgcagaggatcttgaacttctgggcctggaacatcttaaaagtgcttta
aacgatcggggactaaagtgtggcggatctcttgttgaacgcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGACGTGACTGATCTGTTCAATG 457 339 1240 Antisense
AACATCCGAAGCAGTTCCAGAAGTT 178 137 1452 Antisense
CGTCCAAGACTTGATAGCGCTTCTA 74 245 1510 Antisense
GCGCTTCTAACATTGACGATCTTCC 581 287 1526 Antisense
ATCGACGTTAAAACTCCTTGTGAAT 520 33 1555 Antisense
TGAATATGGTCCAATCTCGCTCGAC 703 575 1575 Antisense
GCTCGACGATTTCACCAGTGCAGAG 653 297 1593 Antisense
AGAGGATCTTGAACTTCTGGGCCTG 191 69 1614 Antisense
AACTTCTGGGCCTGGAACATCTTAA 108 139 1625 Antisense
GGGGACTAAAGTGTGGCGGATCTCT 316 499 1667 Antisense
GTGGCGGATCTCTTGTTGAACGCGC 286 491 1679 Antisense
Sequence Source Affymetrix Proprietary Database