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[182753_at]

Description Y43D4A.6 /REP_DB=WormBase Gene ID /WP=CE21879 /TR=Q9NF25 /GB=CAB60347.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 65      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 14.1
(-2.4)
A 		59.5
(0.5)
A 		4.4
(-3)
A 		99.5
(3)
A 		95
B 3
(-3)
A 		35.4
(2.3)
A 		39.7
(3)
A 		10.6
(-1.8)
A 		37
C 32.8
(-1.7)
A 		11.8
(-3)
A 		11.1
(-3)
A 		112.9
(3)
A 		102
avr 16.6
(-3) 		35.6
(-1) 		18.4
(-2.8) 		74.3
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 6.2
(-2.5)
A 		4.2
(-2.7)
A 		33.8
(0.8)
A 		51.9
(3)
A 		22.8
(-0.5)
A 		1.9
(-3)
A 		50
B 10.2
(-2.5)
A 		33.2
(0.6)
A 		17.8
(-1.5)
A 		8.6
(-2.8)
A 		6.8
(-3)
A 		51
(3)
A 		44
C 30.5
(1.8)
A 		12.3
(-2.4)
A 		11
(-2.7)
A 		13.1
(-2.2)
A 		9.6
(-3)
A 		35.6
(3)
A 		26
avr 15.6
(-2.1) 		16.6
(-1.7) 		20.9
(-0.1) 		24.5
(1.2) 		13.1
(-3) 		29.5
(3) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 5.1
(-1.2)
A 		12.9
(2.2)
A 		1.1
(-3)
A 		14.6
(3)
A 		14
B 0.9
(-3)
A 		8.7
(2.6)
A 		9.2
(3)
A 		2.3
(-2)
A 		8
C 10.9
(-0.1)
A 		3.4
(-3)
A 		3.9
(-2.8)
A 		19
(3)
A 		16
avr 5.6
(-2.3) 		8.3
(-0) 		4.7
(-3) 		12
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 1.1
(-2.7)
A 		1.5
(-2.5)
A 		7.4
(0.3)
A 		13.2
(3)
A 		6.9
(0)
A 		0.5
(-3)
A 		13
B 3.5
(-2.6)
A 		11.1
(0.2)
A 		4.3
(-2.3)
A 		2.3
(-3)
A 		2.8
(-2.8)
A 		19
(3)
A 		17
C 11.3
(2.6)
A 		3.7
(-2.3)
A 		2.8
(-2.9)
A 		2.6
(-3)
A 		3.1
(-2.7)
A 		11.9
(3)
A 		9
avr 5.3
(-2) 		5.4
(-1.9) 		4.8
(-2.5) 		6
(-1.4) 		4.3
(-3) 		10.5
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182753_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9939
Sequence Type Exemplar sequence
Representative Public ID Y43D4A.6 NCBI
Target Description Y43D4A.6 /REP_DB=WormBase Gene ID /WP=CE21879 /TR=Q9NF25 /GB=CAB60347.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070588(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070588 NCBI Caenorhabditis elegans Y43D4A.6 (Y43D4A.6) mRNA, complete cds. 11/11 None
SNAP00000037221 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16787286:16788469:1 11/11 None
GENEFINDER00000037224 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16787286:16788469:1 11/11 None
Y43D4A.6 ENSEMBL cdna:known chromosome:CEL140:IV:16787286:16788469:1 gene:Y43D4A.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16787291-16788475(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y43D4A.6
Entrez Gene 189855 Entrez gene
SwissProt Q9NF25 EMBL-EBI
WormBase CE21879 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60347 Hypothetical protein Y43D4A.6 [Caenorhabditis elegans] ref|NP_502989.1| Y43D4A.6 [Caenorhabditis elegans] 1.0E-180
blast AAF59485 Checkpoint kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024233.1| CHeckpoint Kinase family member (chk-1) [Caenorhabditis elegans] sp|Q9N3Z3|CHK1_CAEEL Serine/threonine-protein kinase chk-1 1.0E-62
blast AAA93318 serine/threonine kinase 1.0E-62
Protein Families
Method ID Description E-Value
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 2.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-77
Sequence
Target Sequence 
>C. ELEGANS:182753_AT
atcgcagatttcggactttcaacccgatttattgtggatgacaaggagattctgttgcat
gaaagaattggaactacaccatttatggctccagaactctttgccaaaaagaagcatcgt
ggacctccagtggatatctggtcagctggaattcttgttttctcgttgttaactggaaat
cttccttgggatgagccaaaagaatcagacattctatattctcaatggatgaataataaa
acatctaagaactgtatctggtacggccttagtactccagtagtgagattcctgaagaaa
cttctgaaggaaaatgttaatgaaagagcaacaattgaggaaattgagtcagatccatgg
cttgaagatttggaagaagaaagagagaattgcaccaagttccctacttccacacgtaga
aaaagaagttttccaaaaatcagcagaagaatcacttgctccagtaattcaggacaatca
tctgaacaaagtggatgttctgaaggcccagctgcaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGCAGATTTCGGACTTTCAACCC 73 37 445 Antisense
TATGGCTCCAGAACTCTTTGCCAAA 455 655 528 Antisense
GCATCGTGGACCTCCAGTGGATATC 164 309 558 Antisense
GCTGGAATTCTTGTTTTCTCGTTGT 463 293 589 Antisense
TGGAAATCTTCCTTGGGATGAGCCA 667 547 618 Antisense
GAACTGTATCTGGTACGGCCTTAGT 684 347 693 Antisense
ACGGCCTTAGTACTCCAGTAGTGAG 42 97 707 Antisense
GAAATTGAGTCAGATCCATGGCTTG 201 361 784 Antisense
AGAGAATTGCACCAAGTTCCCTACT 530 69 828 Antisense
GAATCACTTGCTCCAGTAATTCAGG 549 327 893 Antisense
TGTTCTGAAGGCCCAGCTGCAAAAA 659 561 940 Antisense
Sequence Source Affymetrix Proprietary Database