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[182747_at]

Description R08E5.3 /REP_DB=WormBase Gene ID /WP=CE12576 /TR=O01593 /GB=AAB52277.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 52      6grp_total > 52      4grp_total > 33      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1655
1 2 3 4 range
A 266.1
(-3)
P 		1492.4
(1.7)
P 		1111
(0.3)
P 		1820.9
(3)
P 		1555
B 231.1
(-3)
P 		1189.1
(1.3)
P 		1211.4
(1.4)
P 		1567.9
(3)
P 		1337
C 166
(-3)
P 		1212.5
(1.7)
P 		1143.9
(1.4)
P 		1494.7
(3)
P 		1329
avr 221.1
(-3) 		1298
(1.6) 		1155.4
(1) 		1627.8
(3) 		1407

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 799
1 2 3 4 5 6 range
A 613.3
(-0.4)
P 		535.8
(-1.3)
P 		919
(3)
P 		517.6
(-1.5)
P 		408
(-2.7)
P 		385
(-3)
P 		534
B 477.9
(-1.9)
P 		598.5
(-0.2)
P 		828
(3)
P 		638.4
(0.4)
P 		396.9
(-3)
P 		417.7
(-2.7)
P 		431
C 272.4
(-3)
P 		731.8
(0.4)
P 		1071.8
(3)
P 		617.4
(-0.4)
P 		404.5
(-2)
P 		383.3
(-2.2)
P 		799
avr 454.5
(-2.3) 		622
(-0.5) 		939.6
(3) 		591.1
(-0.8) 		403.1
(-2.9) 		395.3
(-3) 		544
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 344
1 2 3 4 range
A 96.9
(-3)
P 		324
(3)
P 		290.4
(2.1)
P 		266.5
(1.5)
P 		227
B 73.7
(-3)
P 		293.7
(2.1)
P 		280.7
(1.8)
P 		334.1
(3)
P 		260
C 54.9
(-3)
P 		348.6
(2.1)
P 		398.7
(3)
P 		252
(0.4)
P 		344
avr 75.2
(-3) 		322.1
(3) 		323.3
(3) 		284.2
(2.1) 		248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 113.1
(-1.9)
P 		190.6
(2.5)
P 		200.1
(3)
P 		131.3
(-0.9)
P 		123.1
(-1.3)
P 		93.7
(-3)
P 		106
B 166.3
(-1.6)
P 		199.2
(2.7)
P 		201.1
(3)
P 		169.1
(-1.2)
P 		161.3
(-2.3)
P 		155.8
(-3)
P 		45
C 101
(-3)
P 		223.3
(1.3)
P 		272.8
(3)
P 		124.2
(-2.2)
P 		130.9
(-2)
P 		128.3
(-2)
P 		172
avr 126.8
(-2.9) 		204.4
(1.8) 		224.7
(3) 		141.5
(-2.1) 		138.4
(-2.2) 		125.9
(-3) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182747_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14904
Sequence Type Exemplar sequence
Representative Public ID R08E5.3 NCBI
Target Description R08E5.3 /REP_DB=WormBase Gene ID /WP=CE12576 /TR=O01593 /GB=AAB52277.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071644(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071644 NCBI Caenorhabditis elegans R08E5.3 (R08E5.3) mRNA, complete cds. 11/11 None
SNAP00000044451 ENSEMBL cdna:SNAP chromosome:CEL140:V:3772003:3774191:1 11/11 None
GENEFINDER00000044456 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3772003:3774248:1 11/11 None
R08E5.3.1 ENSEMBL cdna:known chromosome:CEL140:V:3771663:3775360:1 gene:R08E5.3 11/11 None
R08E5.3.2 ENSEMBL cdna:known chromosome:CEL140:V:3771873:3775360:1 gene:R08E5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3772002-3774248(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R08E5.3
Entrez Gene 178795 Entrez gene
SwissProt O01593 EMBL-EBI
WormBase CE12576 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52277 Hypothetical protein R08E5.3 [Caenorhabditis elegans] ref|NP_504045.1| R08E5.3 [Caenorhabditis elegans] 0.0
blast CAE65471 Hypothetical protein CBG10437 [Caenorhabditis briggsae] 0.0
blast CAE65471 Hypothetical protein CBG10437 [Caenorhabditis briggsae] 1.0E-173
Sequence
Target Sequence 
>C. ELEGANS:182747_AT
cggacatcggcaacggagtcgtcgagaagcttgaagccggcggagtacgagtcctggacg
tcgggtgcggcggtggattccattcttcgctgctcgccgagcaatacccaaagtcgcatt
ttgtcgggctagacattggagaagatgccatccggcaggcgaaacaacgcaagactaaat
caggagcggctttcaacaatctcgaatttattgaatgtgacgccggaaaaatgccagaaa
tctggacggactccttcgatttggtgcttatttttgatgcatgccacgatcagcggagac
cagatttgtgcgtccaagaaatccaccgagttctcaagccaagcgggatgttcgctatgg
tagaggtgctcggctccagtaacgtatacaccgataaagccgccatgggcccattggctg
caatgatgtacgggtgctccatgttccattgtctccctgtcggaagtaactgcccagatg
ctctgtgttatggtgcaatgtgggggcagaaacgtgctaccgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGACATCGGCAACGGAGTCGTCGA 706 241 497 Antisense
GTACGAGTCCTGGACGTCGGGTGCG 665 455 541 Antisense
GGACTCCTTCGATTTGGTGCTTATT 157 525 744 Antisense
CATGCCACGATCAGCGGAGACCAGA 392 211 776 Antisense
TCCACCGAGTTCTCAAGCCAAGCGG 371 587 818 Antisense
TGCTCGGCTCCAGTAACGTATACAC 70 581 863 Antisense
ATACACCGATAAAGCCGCCATGGGC 18 25 882 Antisense
AATGATGTACGGGTGCTCCATGTTC 346 163 918 Antisense
CCATTGTCTCCCTGTCGGAAGTAAC 382 269 942 Antisense
GAAGTAACTGCCCAGATGCTCTGTG 114 337 959 Antisense
TGGGGGCAGAAACGTGCTACCGACC 604 551 997 Antisense
Sequence Source Affymetrix Proprietary Database