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[182735_at]

Description R11B5.1 /REP_DB=WormBase Gene ID /WP=CE04827 /TR=Q21928 /GB=AAA83513.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 82      6grp_total > 37      4grp_total > 21      6grp_highest_sub > Cu      6grp_cgc > 15      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 392.2
(3)
P 		293.2
(-1.1)
P 		245.9
(-3)
P 		351.4
(1.3)
P 		146
B 308.8
(0)
P 		250.4
(-1.8)
P 		214.2
(-3)
P 		401.4
(3)
P 		187
C 328
(-0.2)
P 		280.5
(-1.6)
P 		229.2
(-3)
P 		443.8
(3)
P 		215
avr 343
(1) 		274.7
(-1.4) 		229.8
(-3) 		398.9
(3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 452.8
(3)
P 		438.8
(2.6)
P 		253.3
(-3)
P 		254
(-3)
P 		372
(0.6)
P 		312.9
(-1.2)
P 		200
B 384.8
(3)
P 		360.7
(1.6)
P 		330.1
(-0.1)
P 		383.8
(2.9)
P 		278.2
(-3)
P 		384.4
(3)
P 		107
C 433.7
(2.5)
P 		306
(-0.8)
P 		224.6
(-3)
P 		451.4
(3)
P 		267.8
(-1.9)
P 		359.3
(0.6)
P 		227
avr 423.8
(3) 		368.5
(0.9) 		269.3
(-3) 		363.1
(0.6) 		306
(-1.6) 		352.2
(0.2) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 142.8
(3)
P 		63.6
(-2.2)
P 		64.3
(-2.2)
P 		51.4
(-3)
P 		91
B 98.5
(3)
P 		61.8
(-1.5)
P 		49.6
(-3)
P 		85.5
(1.4)
P 		49
C 108.6
(3)
P 		80.6
(-2)
P 		79.9
(-2.1)
P 		74.8
(-3)
P 		34
avr 116.6
(3) 		68.7
(-2.5) 		64.6
(-3) 		70.6
(-2.3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 83.5
(-1.3)
P 		156
(3)
P 		55.2
(-3)
P 		64.4
(-2.5)
P 		112.3
(0.4)
P 		76.2
(-1.8)
P 		101
B 133.9
(2.1)
P 		120.1
(0.8)
P 		80.1
(-3)
P 		101.7
(-0.9)
P 		113
(0.1)
P 		143.3
(3)
P 		63
C 160.8
(3)
P 		93.4
(-0.9)
P 		57.2
(-3)
P 		90.8
(-1.1)
P 		86.6
(-1.3)
P 		120.3
(0.7)
P 		104
avr 126.1
(3) 		123.2
(2.7) 		64.2
(-3) 		85.6
(-0.9) 		104
(0.9) 		113.3
(1.8) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182735_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18183
Sequence Type Exemplar sequence
Representative Public ID R11B5.1 NCBI
Target Description R11B5.1 /REP_DB=WormBase Gene ID /WP=CE04827 /TR=Q21928 /GB=AAA83513.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076057(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076057 NCBI Caenorhabditis elegans R11B5.1 (R11B5.1) mRNA, complete cds. 11/11 None
SNAP00000019674 ENSEMBL cdna:SNAP chromosome:CEL140:X:2525716:2529893:-1 11/11 None
GENEFINDER00000019675 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2525716:2532387:-1 11/11 None
R11B5.1 ENSEMBL cdna:known chromosome:CEL140:X:2525207:2532430:-1 gene:R11B5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2525715-2530751(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R11B5.1
Entrez Gene 180556 Entrez gene
SwissProt Q21928 EMBL-EBI
WormBase CE28759 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.12901.1.S1_AT similar to TBC1 domain family member 12 cfa
HG-U133_PLUS_2:221858_AT TBC1 domain family, member 12 hs
HG-U133A_2:221858_AT TBC1 domain family, member 12 hs
HG-U133A:221858_AT TBC1 domain family, member 12 hs
HU35KSUBC:RC_N40192_AT TBC1 domain family, member 12 hs
HU35KSUBD:RC_F02202_AT TBC1 domain family, member 12 hs
U133_X3P:HS2.407094.1.S1_3P_S_AT TBC1 domain family, member 12 hs
U133_X3P:HS.100960.0.S1_3P_AT TBC1 domain family, member 12 hs
HG-U133_PLUS_2:1557609_S_AT TBC1 domain family, member 12 hs
HG-U95AV2:36320_AT TBC1 domain family, member 12 hs
HG-U95B:48015_AT TBC1 domain family, member 12 hs
MG-U74BV2:106140_AT TBC1D12: TBC1 domain family, member 12 mm
MOE430B:1435675_AT TBC1D12: TBC1 domain family, member 12 mm
MOUSE430_2:1435675_AT TBC1D12: TBC1 domain family, member 12 mm
MOUSE430_2:1443297_AT TBC1D12: TBC1 domain family, member 12 (Tbc1d12), mRNA mm
MOE430B:1443297_AT TBC1D12: TBC1 domain family, member 12 (Tbc1d12), mRNA mm
MG-U74BV2:106172_AT TBC1D12: TBC1 domain family, member 12 (Tbc1d12), mRNA mm
MOE430B:1444221_AT TBC1D12: TBC1 domain family, member 12 (Tbc1d12), mRNA mm
MOUSE430_2:1444221_AT TBC1D12: TBC1 domain family, member 12 (Tbc1d12), mRNA mm
Protein Similarities
Method ID Description E-Value
blast AAA83513 Hypothetical protein R11B5.1 [Caenorhabditis elegans] ref|NP_508458.1| R11B5.1 [Caenorhabditis elegans] 0.0
blast CAE58864 Hypothetical protein CBG02093 [Caenorhabditis briggsae] 0.0
blast XP_585982 PREDICTED: similar to TBC1 domain family member 12 [Bos taurus] 2.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.4E-8
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.5E-11
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.4E-8
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.5E-11
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.4E-8
Pfam IPR000195 EMBL-EBI RabGAP/TBC domain 2.5E-11
Sequence
Target Sequence 
>C. ELEGANS:182735_AT
catacccggcattcatatcgtttgccaatttgctcgacagatccctccaatctgcatttt
tcggactcaaacaacctcagatgacggagtattttattgcgtatgatcgatacttggagc
aagaacttccggctcttcatcagcatttggacaaactcgacgtccgcccggatctctacc
tcattgaatggacgtttgcaatgtacgccaaatcgttaccgctagacgtgacctgccgta
tttgggatgtgtattttcgtgacggcgaggagtttttgttcaaggcagcactcggcattc
ttcgtatgtacgagcccaaactgctgactatggattttgatgactgtgtggaatttttga
cgaaacttcccaacacgcttaccggcgccgagctttttcgaaacattgagccatttatga
gaccgtacaatggagaatcgtctcggtccaagaagcgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATACCCGGCATTCATATCGTTTGC 194 215 965 Antisense
GTTTGCCAATTTGCTCGACAGATCC 274 451 984 Antisense
ATCCCTCCAATCTGCATTTTTCGGA 328 37 1005 Antisense
TCCGGCTCTTCATCAGCATTTGGAC 48 595 1092 Antisense
CCCGGATCTCTACCTCATTGAATGG 585 259 1131 Antisense
GTACGCCAAATCGTTACCGCTAGAC 328 457 1167 Antisense
GTGACCTGCCGTATTTGGGATGTGT 602 479 1192 Antisense
TTGTTCAAGGCAGCACTCGGCATTC 116 703 1240 Antisense
GTACGAGCCCAAACTGCTGACTATG 357 459 1272 Antisense
TTTTGACGAAACTTCCCAACACGCT 586 671 1319 Antisense
AATCGTCTCGGTCCAAGAAGCGCTT 248 169 1400 Antisense
Sequence Source Affymetrix Proprietary Database