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[182730_at]

Description K08D9.5 /REP_DB=WormBase Gene ID /WP=CE19985 /TR=Q9TXN3 /GB=AAC69355.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 71      4grp_total > 35      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 34.6
(-3)
A 		47.4
(1.2)
A 		40
(-1.2)
A 		53
(3)
A 		18
B 7
(-3)
A 		75.6
(3)
A 		31.4
(-0.9)
A 		21.5
(-1.7)
A 		69
C 47.3
(-0.3)
A 		74.2
(3)
M 		25.8
(-3)
A 		54.6
(0.6)
A 		48
avr 29.6
(-3) 		65.7
(3) 		32.4
(-2.5) 		43
(-0.8) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 33.7
(-2.2)
A 		25.1
(-2.7)
A 		77.6
(0.8)
P 		109.3
(3)
P 		69.9
(0.3)
A 		21.4
(-3)
A 		88
B 49.2
(-0)
A 		21.9
(-2.3)
A 		52.8
(0.2)
A 		68.4
(1.5)
P 		86
(3)
A 		13.6
(-3)
A 		72
C 41.4
(-1.7)
A 		32.4
(-2.9)
A 		57.5
(0.4)
A 		77.7
(3)
A 		45.1
(-1.2)
A 		31.3
(-3)
A 		46
avr 41.4
(-1.2) 		26.5
(-2.6) 		62.6
(0.9) 		85.1
(3) 		67
(1.3) 		22.1
(-3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 12.6
(3)
A 		10.3
(0.1)
A 		10.4
(0.3)
A 		7.8
(-3)
A 		5
B 2.2
(-3)
A 		18.7
(3)
A 		7.3
(-1.1)
A 		4.6
(-2.1)
A 		17
C 15.7
(0.3)
A 		21.3
(3)
M 		9
(-3)
A 		9.2
(-2.9)
A 		12
avr 10.2
(-1.1) 		16.8
(3) 		8.9
(-1.9) 		7.2
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 6.2
(-2.7)
A 		8.9
(-2)
A 		16.9
(0.1)
P 		27.7
(3)
P 		21.1
(1.2)
A 		5.2
(-3)
A 		23
B 17.1
(-0.6)
A 		7.3
(-2.6)
A 		12.8
(-1.4)
A 		18.1
(-0.4)
P 		34.9
(3)
A 		5.1
(-3)
A 		30
C 15.4
(2.8)
A 		9.9
(-3)
A 		14.6
(1.9)
A 		15.6
(3)
A 		14.6
(1.9)
A 		10.5
(-2.4)
A 		6
avr 12.9
(-0.8) 		8.7
(-2.3) 		14.8
(-0.1) 		20.5
(1.9) 		23.5
(3) 		6.9
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182730_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15434
Sequence Type Exemplar sequence
Representative Public ID K08D9.5 NCBI
Target Description K08D9.5 /REP_DB=WormBase Gene ID /WP=CE19985 /TR=Q9TXN3 /GB=AAC69355.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071482(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071482 NCBI Caenorhabditis elegans K08D9.5 (K08D9.5) mRNA, complete cds. 11/11 None
SNAP00000017185 ENSEMBL cdna:SNAP chromosome:CEL140:V:3223441:3226433:-1 10/11 None
GENEFINDER00000017192 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3223441:3226885:-1 11/11 None
K08D9.5 ENSEMBL cdna:known chromosome:CEL140:V:3223441:3226885:-1 gene:K08D9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3223440-3226885(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08D9.5
Entrez Gene 187148 Entrez gene
SwissProt Q9TXN3 EMBL-EBI
WormBase CE19985 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69355 Hypothetical protein K08D9.5 [Caenorhabditis elegans] ref|NP_503883.1| K08D9.5 [Caenorhabditis elegans] 0.0
blast AAC69355 Hypothetical protein K08D9.5 [Caenorhabditis elegans] ref|NP_503883.1| K08D9.5 [Caenorhabditis elegans] 1.0E-144
blast AAC69355 Hypothetical protein K08D9.5 [Caenorhabditis elegans] ref|NP_503883.1| K08D9.5 [Caenorhabditis elegans] 6.0E-80
blast AAY43981 Hypothetical protein ZK381.8 [Caenorhabditis elegans] ref|NP_001023614.1| ZK381.8 [Caenorhabditis elegans] 9.0E-49
blast AAY43981 Hypothetical protein ZK381.8 [Caenorhabditis elegans] ref|NP_001023614.1| ZK381.8 [Caenorhabditis elegans] 2.0E-48
blast CAE61768 Hypothetical protein CBG05728 [Caenorhabditis briggsae] 3.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 7.2E-29
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.8E-13
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 7.2E-29
Sequence
Target Sequence 
>C. ELEGANS:182730_AT
aattttcacgacctcatgtctacattaaaatcatcggtttcaaaaggatcaaaaccggtg
atcaagccggaatttttcaacattattggaattcatgaaaatactaatagttttaataga
cgtttcataacaaccccgtcaattatccacgtggaacgaccaatttcaaagcaggaactc
ggcgagctgcgacaattttggaattttgatttcagcccatcacaagagaaaatccaacct
ggcgatattcaagcactggaagaggatttttggagaatccgaaacatgacaacagttgca
caactcgatggctcacttcccggcagtgatcaccttttcacagcgatttcaaaatgtctc
gcagatcaacgttccgaagaacaactgtgtccgaactttgaaaaatgcgaaattccgcag
aatgctcaaaaatgcttgcacacagatgccaattactataccagcaatcacatggctccg
tacacttatcatttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTTTCACGACCTCATGTCTACAT 104 175 469 Antisense
TAACAACCCCGTCAATTATCCACGT 154 191 596 Antisense
AAAGCAGGAACTCGGCGAGCTGCGA 104 121 636 Antisense
GGAATTTTGATTTCAGCCCATCACA 502 533 668 Antisense
CCAACCTGGCGATATTCAAGCACTG 81 277 702 Antisense
ACAGTTGCACAACTCGATGGCTCAC 697 107 760 Antisense
TGATCACCTTTTCACAGCGATTTCA 481 563 795 Antisense
ATGTCTCGCAGATCAACGTTCCGAA 458 47 822 Antisense
GAAGAACAACTGTGTCCGAACTTTG 203 337 844 Antisense
ATACCAGCAATCACATGGCTCCGTA 14 23 926 Antisense
ATGGCTCCGTACACTTATCATTTGT 442 53 940 Antisense
Sequence Source Affymetrix Proprietary Database