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[182728_at]

Description C50F2.4 /REP_DB=WormBase Gene ID /WP=CE08910 /TR=P91177 /GB=AAB37796.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 44      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 194.9
(3)
P 		118.8
(-2.1)
A 		114.1
(-2.4)
A 		105.6
(-3)
A 		89
B 120.4
(-1.3)
P 		229.1
(3)
A 		159.1
(0.2)
A 		78.1
(-3)
A 		151
C 178.3
(0.6)
P 		224.5
(3)
A 		114.4
(-2.7)
A 		107.6
(-3)
P 		117
avr 164.5
(1.3) 		190.8
(3) 		129.2
(-0.9) 		97.1
(-3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 12.3
(-3)
A 		94.2
(1.1)
A 		105.9
(1.7)
P 		64.1
(-0.4)
A 		131.8
(3)
A 		108.1
(1.8)
A 		120
B 154
(3)
A 		103.5
(-0.1)
A 		127.4
(1.3)
P 		57.3
(-3)
A 		102.1
(-0.2)
A 		94.7
(-0.7)
P 		97
C 71.5
(-3)
A 		78.1
(-2.3)
A 		81.5
(-2)
A 		132
(3)
A 		104.8
(0.3)
A 		124.8
(2.3)
P 		61
avr 79.3
(-3) 		91.9
(-0.7) 		104.9
(1.6) 		84.5
(-2.1) 		112.9
(3) 		109.2
(2.3) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 71
(3)
P 		25.8
(-1.9)
A 		29.8
(-1.5)
A 		15.5
(-3)
A 		56
B 38.4
(0.3)
P 		56.6
(3)
A 		36.9
(0)
A 		16.6
(-3)
A 		40
C 59
(2.3)
P 		64.5
(3)
A 		39.9
(-0.2)
A 		18.1
(-3)
P 		46
avr 56.1
(3) 		49
(1.9) 		35.5
(-0.1) 		16.7
(-3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 2.3
(-3)
A 		33.5
(2)
A 		23.1
(0.3)
P 		16.3
(-0.8)
A 		39.8
(3)
A 		26.3
(0.8)
A 		38
B 53.6
(3)
A 		34.4
(-0)
A 		30.9
(-0.5)
P 		15.2
(-3)
A 		41.5
(1.1)
A 		35.3
(0.1)
P 		38
C 26.5
(-1.4)
A 		23.8
(-2.1)
A 		20.7
(-3)
A 		26.5
(-1.4)
A 		33.9
(0.8)
A 		41.8
(3)
P 		21
avr 27.5
(-0.4) 		30.6
(0.5) 		24.9
(-1.2) 		19.3
(-3) 		38.4
(3) 		34.5
(1.8) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182728_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.258
Sequence Type Exemplar sequence
Representative Public ID C50F2.4 NCBI
Target Description C50F2.4 /REP_DB=WormBase Gene ID /WP=CE08910 /TR=P91177 /GB=AAB37796.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058853(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058853 NCBI Caenorhabditis elegans C50F2.4 (C50F2.4) mRNA, complete cds. 11/11 None
SNAP00000013848 ENSEMBL cdna:SNAP chromosome:CEL140:I:3887166:3891520:-1 11/11 None
GENEFINDER00000013859 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3889131:3891520:-1 11/11 None
C50F2.4 ENSEMBL cdna:known chromosome:CEL140:I:3889131:3891520:-1 gene:C50F2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3889130-3891520(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50F2.4
Entrez Gene 183674 Entrez gene
SwissProt P91177 EMBL-EBI
WormBase CE08910 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37796 Hypothetical protein C50F2.4 [Caenorhabditis elegans] ref|NP_491254.1| C50F2.4 [Caenorhabditis elegans] 4.0E-97
blast AAB37796 Hypothetical protein C50F2.4 [Caenorhabditis elegans] ref|NP_491254.1| C50F2.4 [Caenorhabditis elegans] 7.0E-88
blast CAE66645 Hypothetical protein CBG11982 [Caenorhabditis briggsae] 3.0E-79
blast CAE66645 Hypothetical protein CBG11982 [Caenorhabditis briggsae] 5.0E-70
Sequence
Target Sequence 
>C. ELEGANS:182728_AT
attttccttgttttctgtgattcttgttgtggtggctgatgagaatgatgtacaggtaaa
accagcattaagtcaatgcaaaagggtcaacccatgcggagccacaatttccacatatcc
actcttggagccggacgctgaagaagcttcgggattcggtagcggtgcggtaccagttta
tgatgaaattttgacgcatgaaatctacgaccttaacgccacccccgaaatgcacaactt
cacactttgctcgtgtccagacgatggtgtctgcgattttgagtcggattcaaatatggt
caaggtcgattaccacgttacactgcgattctgtaatttggagacgatatcgtcaacatg
taaacgctatgacgttaatttgagagttattggggcagcacagccgtctggagacagcgt
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTTCCTTGTTTTCTGTGATTCTT 256 15 39 Antisense
TCAACCCATGCGGAGCCACAATTTC 347 631 125 Antisense
CACAATTTCCACATATCCACTCTTG 468 193 141 Antisense
GGAGCCGGACGCTGAAGAAGCTTCG 62 523 165 Antisense
GATTCGGTAGCGGTGCGGTACCAGT 279 415 191 Antisense
TGAAATCTACGACCTTAACGCCACC 507 569 237 Antisense
AATGCACAACTTCACACTTTGCTCG 649 165 267 Antisense
CACTTTGCTCGTGTCCAGACGATGG 263 197 281 Antisense
GACGATGGTGTCTGCGATTTTGAGT 304 379 298 Antisense
GATTACCACGTTACACTGCGATTCT 679 429 346 Antisense
AGCACAGCCGTCTGGAGACAGCGTG 272 77 435 Antisense
Sequence Source Affymetrix Proprietary Database