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[182727_at]

Description F48B9.8 /REP_DB=WormBase Gene ID /WP=CE04616 /TR=Q20557 /GB=AAA81753.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 11      4grp_high_8_8 > 39      6grp_highest_nonorm > 71      6grp_total > 60      4grp_total > 40      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 268.5
(-3)
P 		373.1
(3)
P 		336.1
(0.9)
P 		269.5
(-2.9)
P 		105
B 285.5
(-1.8)
P 		260
(-3)
P 		386.1
(3)
P 		301.6
(-1)
P 		126
C 266.6
(-3)
P 		345.5
(1.3)
P 		338.8
(1)
P 		375.6
(3)
P 		109
avr 273.5
(-3) 		326.2
(0.9) 		353.7
(3) 		315.6
(0.1) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 330
1 2 3 4 5 6 range
A 360.6
(-0.2)
P 		308
(-1.4)
P 		456.8
(2)
P 		500.1
(3)
P 		300.1
(-1.6)
P 		238.8
(-3)
P 		261
B 379.7
(-1.3)
P 		404.3
(-0.7)
P 		568.5
(3)
P 		435
(-0)
P 		369
(-1.5)
P 		302.8
(-3)
P 		266
C 291.3
(-2.4)
P 		355.5
(-0.8)
P 		329.7
(-1.5)
P 		515.9
(3)
P 		265.5
(-3)
P 		287.7
(-2.5)
P 		250
avr 343.9
(-1) 		355.9
(-0.7) 		451.7
(2.1) 		483.7
(3) 		311.5
(-2) 		276.4
(-3) 		207
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 97.8
(3)
P 		81
(1.3)
P 		87.9
(2)
P 		39.4
(-3)
P 		58
B 91
(3)
P 		64.2
(-3)
P 		89.5
(2.7)
P 		64.3
(-3)
P 		27
C 88.3
(-0.3)
P 		99.3
(0.9)
P 		118.1
(3)
P 		63.3
(-3)
P 		55
avr 92.4
(2.1) 		81.5
(0.6) 		98.5
(3) 		55.7
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 66.5
(-2.3)
P 		109.6
(1.5)
P 		99.5
(0.6)
P 		126.8
(3)
P 		90.6
(-0.2)
P 		58.1
(-3)
P 		69
B 132.1
(0.1)
P 		134.6
(0.5)
P 		138
(1.1)
P 		115.2
(-2.6)
P 		149.9
(3)
P 		112.9
(-3)
P 		37
C 108
(2.9)
P 		108.5
(3)
P 		83.9
(-3)
P 		103.7
(1.8)
P 		85.9
(-2.5)
P 		96.3
(0)
P 		25
avr 102.2
(-0.2) 		117.6
(3) 		107.1
(0.8) 		115.2
(2.5) 		108.8
(1.1) 		89.1
(-3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182727_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18501
Sequence Type Exemplar sequence
Representative Public ID F48B9.8 NCBI
Target Description F48B9.8 /REP_DB=WormBase Gene ID /WP=CE04616 /TR=Q20557 /GB=AAA81753.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076001(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076001 NCBI Caenorhabditis elegans F48B9.8 (F48B9.8) mRNA, complete cds. 11/11 None
SNAP00000005470 ENSEMBL cdna:SNAP chromosome:CEL140:X:2174022:2176400:-1 11/11 None
GENEFINDER00000005474 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2174022:2176400:-1 11/11 None
F48B9.8 ENSEMBL cdna:known chromosome:CEL140:X:2174022:2176382:-1 gene:F48B9.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2174021-2176382(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F48B9.8
Entrez Gene 185970 Entrez gene
SwissProt Q20557 EMBL-EBI
WormBase CE04616 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81753 Hypothetical protein F48B9.8 [Caenorhabditis elegans] ref|NP_508402.1| F48B9.8 [Caenorhabditis elegans] 1.0E-134
blast CAE68344 Hypothetical protein CBG14070 [Caenorhabditis briggsae] 7.0E-56
blast CAE68344 Hypothetical protein CBG14070 [Caenorhabditis briggsae] 9.0E-56
blast XP_798618 PREDICTED: similar to Autosomal Highly Conserved Protein [Strongylocentrotus purpuratus] 3.0E-10
Sequence
Target Sequence 
>C. ELEGANS:182727_AT
gagcaggtctttttctgccgaaacttttcaacaaaacgttaacgtagaagttctccaacc
agcccaaccacgtgttgattcagttcaatttgaagctgcaagcttcttgagacgtcttct
ggcagaattgatagattttgtttttattctcagcttcaaacttgctgtagttggaacact
ctcggaattgggtcacattgacctaaagcaaatcggagcaagcttggatgatgatcagga
tgtgatgaacttcattaatcttgctcaagaccttctcccagttgaagttgtgtgtaaagt
attttgctgtttcgtggaagcggtgttaatggctcgaggatttggaccaatcggagttgg
acagacgttgggaaaatggatgtgtggaattcgagtaatttcttgtcgtgatgttacttc
cgcagaacgccctgatcaaattattgtagagggacccgatttgattacttattcacaggc
cctgaagcgatcattcataaaaaacttggtggtcagtagctttttcccactgagcaccgc
agttttccagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCAGGTCTTTTTCTGCCGAAACT 605 389 189 Antisense
CCAGCCCAACCACGTGTTGATTCAG 420 273 247 Antisense
GCAAGCTTCTTGAGACGTCTTCTGG 329 321 286 Antisense
GGAACACTCTCGGAATTGGGTCACA 333 529 361 Antisense
TAATCTTGCTCAAGACCTTCTCCCA 321 635 444 Antisense
CTTGTCGTGATGTTACTTCCGCAGA 710 217 590 Antisense
TTCCGCAGAACGCCCTGATCAAATT 336 697 606 Antisense
GAGGGACCCGATTTGATTACTTATT 334 401 637 Antisense
TCACAGGCCCTGAAGCGATCATTCA 383 625 661 Antisense
GTCAGTAGCTTTTTCCCACTGAGCA 179 467 700 Antisense
CACTGAGCACCGCAGTTTTCCAGAT 182 199 716 Antisense
Sequence Source Affymetrix Proprietary Database